Fluorescein-12-dUTP (1mM, Nuclease free)
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产品编号 D7333-20μl
数量
¥ 668.00
产品包装:
20μl 100μl 500μl
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CAS Number Chemical Formula Molecular Weight Purity Grade
N/A C39H41N4O21P3 (free acid) 994.69 (free acid) ≥95% BioReagent

基本信息(General Information):

Name (Chinese) 荧光素-12-脱氧尿苷三磷酸; 荧光素-12-dUTP
Name (English) Fluorescein-12-dUTP; Fluorescein-X-(5-aminoallyl)-dUTP; 5/6-Fluorescein-X-(5-aminoallyl)-2'-deoxyuridine-5'-triphosphate
Specifications BioReagent, ≥95%
Chemical Formula C39H41N4O21P3 (free acid)
Concentration 1mM
Appearance Clear solution
Structure

产品描述(Description):

General Description Fluorescein-12-dUTP在大多数情况下可以取代dTTP作为各种DNA合成酶、修饰酶、聚合酶,包括Taq DNA Polymerase (D7209)、phi29 DNA Polymerase (D7053)、Klenow Fragment, Exo- (D7039/D7041)、Klenow Fragment (D7037)、Terminal Deoxynucleotidyl Transferase (D7092/D7095)、DNA Polymerase I、逆转录酶(D7188/D7176/D7160/D7153)等的底物,对DNA实现绿色荧光标记。例如通过PCR等生成Fluorescein标记DNA绿色荧光探针,经凝胶电泳后,Fluorescein标记DNA的荧光在激发后清晰可见,无需进一步染色。由该方法生成的荧光标记探针适用于多种应用,如FISH、微阵列、印迹检测、多色荧光分析等。激发/发射波长分别为495/521nm。
Application DNA labeling and detection
包装清单:
产品编号 产品名称 包装
D7333-20µl Fluorescein-12-dUTP (1mM, Nuclease free) 20µl
D7333-100µl Fluorescein-12-dUTP (1mM, Nuclease free) 100µl
D7333-500µl Fluorescein-12-dUTP (1mM, Nuclease free) 500µl
说明书 1份
保存条件:

-20ºC避光保存,至少两年有效。

注意事项:

本产品需避光保存,防止荧光淬灭。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

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