QuickBlock™ Western封闭液
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产品编号 P0252-100ml
数量
¥ 123.00
产品包装:
15ml 100ml 500ml
产品简介
使用说明
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碧云天生产的QuickBlock™ Western封闭液(QuickBlock™ Blocking Buffer for Western Blot)是最新一代的快速高效Western封闭液,总体效果显著优于传统的基于BSA(牛血清白蛋白)、脱脂奶粉、酪蛋白(Casein)等的封闭液及国外同类产品,主要用于Western blot (WB)实验中PVDF膜或硝酸纤维素膜(nitrocellulose membrane, NC膜)的封闭,也可以用于Western实验中一抗或二抗的稀释。
QuickBlock™ Western封闭液快速高效。封闭时间通常仅需5-15分钟,并且和BSA、脱脂奶粉、酪蛋白等传统的Western封闭液以及国外同类的快速封闭液相比,显示出更强的信噪比(参考图1)。
QuickBlock™ Western封闭液封闭后背景极低。本封闭液不含血清和白蛋白,确保极高的信噪比。
QuickBlock™ Western封闭液兼容性好,兼容辣根过氧化物酶(Horseradish peroxidase, HRP)、碱性磷酸酶(Alkaline phosphatase, AP)和生物素标记的二抗。本产品中添加了不影响HRP和AP活性的防腐剂,不会干扰HRP或AP标记二抗的检测。同时本产品不含生物素,不会干扰基于生物素的检测。
QuickBlock™ Western封闭液使用便捷。本产品无需添加任何额外的试剂,可以直接用于印迹膜的封闭。
本产品与BSA及国外同类产品的封闭效果对比参见图1。在相同样品和实验条件下,仅封闭液及封闭时间存在如下图所示的差异时,碧云天的QuickBlock™ Western封闭液封闭后的整体背景明显低于BSA封闭后的背景,而且目的条带亮度明显高于国外同类品牌产品。
图1. QuickBlock™ Western封闭液与BSA及国外同类产品的封闭效果比较。每组实验从左到右依次为:5µl蛋白Marker,2.5µg蛋白量的HeLa细胞裂解液,5µg蛋白量的HeLa细胞裂解液。请注意对于Antibody A,QuickBlock™封闭液比BSA和国外同类产品具有更低的背景,并且和国外同类产品相比信号明显更强。对于Antibody B,QuickBlock™封闭液比BSA具有更低的背景,和国外同类产品相比信号明显更强。实际实验结果会因样品、抗体、实验条件等的不同而存在差异,图中数据仅供参考。

关于不同封闭液的比较和选择,请参考碧云天的相关网页:http://www.beyotime.com/support/blocking-buffer.htm
按照每张膜封闭需要5-10ml QuickBlock™ Western封闭液计算,一个100ml包装的本产品可以封闭10-20张膜,一个500ml包装的本产品可以封闭50-100张膜。
包装清单:

产品编号 产品名称 包装
P0252-100ml QuickBlock™ Western封闭液 100ml
P0252-500ml QuickBlock™ Western封闭液 500ml
说明书 1份

保存条件:
4℃保存,一年有效。长期不使用可以-20℃保存。
注意事项:
通常本产品用于PVDF膜及NC膜时的封闭时间为5-15分钟。对于一些背景非常高的抗体,可以尝试将封闭时间延长为30-60分钟。此外,如有特殊需要,也完全可以4℃封闭过夜。
由于没有任何一种封闭液是适用于所有实验体系的,因此对于一些特殊的实验,可能需要根据具体情况考虑使用其它更合适的封闭液。
取放PVDF膜和NC膜应使用平头镊子,并仅轻轻夹取其边角,操作过程须避免膜表面产生划痕、折痕或压痕等痕迹。
PVDF膜一经浸润和活化,需一直保持湿润,根据Western进行到的具体步骤可放置于western转膜液或洗涤液等适当溶液中,否则可能会产生难以封闭的异常背景。
为进一步提高信噪比,推荐同时使用QuickBlock™Western一抗稀释液(P0256)QuickBlock™ Western二抗稀释液(P0258)进行一抗及二抗的稀释。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 完成转膜后,用Western洗涤液洗涤印迹膜1-2分钟。
2. 根据膜的大小,在平皿或者其它适当容器中倒入一定体积的QuickBlock™ Western封闭液,确保封闭液后续能充分覆盖膜即可。对于常规的western,一张约6.6×8.5cm的膜推荐使用约10ml左右的封闭液。
3. 用平头镊夹住膜的一角,将膜放置在QuickBlock™ Western封闭液中,使封闭液完全浸没膜,置于水平摇床上封闭约10分钟(通常5-15分钟均可;经多种抗体的测试封闭10分钟的效果很多时候会显著优于常规的BSA封闭1小时的效果)。
4. 封闭后的膜即可用于一抗孵育等后续实验。详细的Western操作可以参考如下的相关网页:
http://www.beyotime.com/support/western.htm

相关产品:

产品编号 产品名称 包装
P0023A-100ml Western一抗稀释液 100ml
P0023A-500ml Western一抗稀释液 500ml
P0023B-100ml Western封闭液 100ml
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P0231 QuickBlock™封闭液(TBSTw) 100ml
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P0235 QuickBlock™封闭液(10X)  100ml
P0252-100ml QuickBlock™ Western封闭液 100ml
P0252-500ml QuickBlock™ Western封闭液 500ml
P0256-100ml QuickBlock™ Western一抗稀释液 100ml
P0256-500ml QuickBlock™ Western一抗稀释液 500ml
P0258-100ml QuickBlock™ Western二抗稀释液 100ml
P0258-500ml QuickBlock™ Western二抗稀释液 500ml


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