Western封闭液
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产品编号 P0023B-500ml
数量
¥ 503.00
产品包装:
100ml 500ml
产品简介
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碧云天生产的Western封闭液(Blocking Buffer)可以用于Western时转移了蛋白样品的PVDF膜或NC膜的封闭。封闭后,可以减小后续的一抗或二抗和膜的非特异性结合,降低背景,增强信噪比。
本产品的主要有效成分为经过反复优化的适量BSA和适当的去垢剂等,一方面能有效封闭膜上的蛋白结合位点,另一方面能有效减少后续一抗和二抗的非特异性结合。
本产品可以用于一抗的稀释,但更推荐使用Western检测时一抗稀释专用的Western一抗稀释液(P0023A)QuickBlock™ Western封闭液(P0252)
关于不同封闭液的比较和选择,请参考碧云天的相关网页:http://www.beyotime.com/support/blocking-buffer.htm
按照每张膜封闭需要5-10毫升封闭液计算,一个100ml包装的Western封闭液可以封闭10-20张膜,一个500ml包装的Western封闭液可以封闭50-100张膜。
包装清单:

产品编号 产品名称 包装
P0023B-100ml Western封闭液 100ml
P0023B-500ml Western封闭液 500ml
说明书 1份

保存条件:
4℃保存,一年有效。长期不使用可以-20℃保存。
注意事项:
通常在室温封闭60分钟即可。如果对于一些背景非常高的抗体,可以4℃封闭过夜。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 完成转膜后,用Western洗涤液洗涤蛋白膜1-2分钟。加入Western封闭液,封闭60分钟,然后进行一抗孵育等后续操作。
2. 详细的Western操作可以参考我们的相关网页:http://www.beyotime.com/support/western.htm

相关产品:

产品编号 产品名称 包装
P0023A-100ml Western一抗稀释液 100ml
P0023A-500ml Western一抗稀释液 500ml
P0023B-100ml Western封闭液 100ml
P0023B-500ml Western封闭液 500ml
P0023D-100ml Western二抗稀释液 100ml
P0023D-500ml Western二抗稀释液 500ml
P0220 QuickBlock™封闭液(PBS) 100ml
P0222 QuickBlock™封闭液(PBSTw)  100ml
P0226 QuickBlock™封闭液(PBSTx) 100ml
P0228 QuickBlock™封闭液(TBS) 100ml
P0231 QuickBlock™封闭液(TBSTw) 100ml
P0233 QuickBlock™封闭液(TBSTx)  100ml
P0235 QuickBlock™封闭液(10X)  100ml
P0252-100ml QuickBlock™ Western封闭液 100ml
P0252-500ml QuickBlock™ Western封闭液 500ml
P0256-100ml QuickBlock™ Western一抗稀释液 100ml
P0256-500ml QuickBlock™ Western一抗稀释液 500ml
P0258-100ml QuickBlock™ Western二抗稀释液 100ml
P0258-500ml QuickBlock™ Western二抗稀释液 500ml


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