化学信息：
| 化学名 | 6-bromo-N-prop-2-enylquinazolin-4-amine |
|
| 简称 | SMER28 | |
| 别名 |
AC1LTGZ0, SMER26 |
|
| 中文名 | N/A | |
| 化学式 | C11H10BrN3 | |
| 分子量 | 264.12 | |
| CAS号 | 307538-42-7 | |
| 纯度 | 98% | |
| 溶剂/溶解度 |
Water<1mg/ml; DMSO52mg/ml; Ethanol52mg/ml |
|
| 溶液配制 | 5mg加入1.89ml DMSO，或者每2.64mg加入1ml DMSO，配制成10mM溶液。SC5502-10mM用DMSO配制。 |
生物信息：
| 产品描述 | SMER28 is a small-molecule enhancer (SMER) of autophagy, inducing autophagy independently of rapamycin in mammalian cells. | ||||
| 信号通路 | Autophagy | ||||
| 靶点 | Autophagy | － | － | － | － |
| IC50 | － | － | － | － | － |
| 体外研究 | SMER28 is a positive regulator of autophagy in a mechanism independent from the mTOR pathway. It increases autophagosome synthesis and enhances the clearance of model autophagy substrates such as A53T a-synuclein and mutant huntingtin fragments. A marked decrease in Aβ/APP-CTF levels and enhancement for degradation of Aβ/APP-CTF via autophagy is also induced by SMER28. APP-CTF and LC3-II are cocompartmentalized on SMER28 treatment. | ||||
| 体内研究 | N/A | ||||
| 临床实验 | N/A | ||||
| 特征 | N/A | ||||
相关实验数据(此数据来自于公开文献，碧云天并不保证其有效性)：
| 酶活性检测实验 | |
| 方法 | N/A |
| 细胞实验 | |
| 细胞系 | MEF cells overexpressing βCTF/ N2a-APP cells/ Rat primary neuronal cultures |
| 浓度 | 10μM in MEF cells/0-50μM in N2a-APP cells and rat primary neuronal cultures |
| 处理时间 | 16hrs |
| 方法 | MEF cells overexpressing βCTF are treated with autophagy-enhancing compounds (10μM) for 16 h. Cell lysates are analyzed by SDS-PAGE and Western blotting using anti-βCTF (6E10) and anti-tubulin antibodies. |
| 动物实验 | |
| 动物模型 | N/A |
| 配制 | N/A |
| 剂量 | N/A |
| 给药方式 | N/A |
| 产品编号 | 产品名称 | 包装 |
| SC5502-10mM | SMER28 (Autophagy增强剂) | 10mM×0.2ml |
| SC5502-5mg | SMER28 (Autophagy增强剂) | 5mg |
| SC5502-25mg | SMER28 (Autophagy增强剂) | 25mg |
| — | 说明书 | 1份 |
保存条件：
-20℃保存，至少一年有效。5mg和25mg包装也可室温保存，至少6个月有效。如果溶于非DMSO溶剂，建议分装后-80℃保存，预计6个月内有效。
