pLenti-PSKH2-sgRNA (PSKH2基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。
碧云天同时提供基于CRISPR/Cas9技术的PSKH2基因敲除的质粒(L30020 pLenti-PSKH2-sgRNA)、慢病毒(L30021 PSKH2 Knockout Lentivirus)、HEK293T细胞(L30022 PSKH2 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L30023 PSKH2 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L30024 PSKH2 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
PSKH2基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | PSKH2 | 85481 | BC126180 | NM_033126 |
| About the gene | |
| Official Symbol | PSKH2 |
| Previous Symbol | - |
| Official Full Name | protein serine kinase H2 |
| Synonyms | - |
| Location | 8q21.3 |
| Gene Type | protein-coding gene |
| Uniprot ID | Q96QS6 |
| Pathway/Library | others |
| Gene Summary | On the human kinome tree, a distinct ‘dark’ pseudokinase, termed PSKH2, is also most similar to canonical members of the CAMK1/2 arm of the kinome, where it forms a two‐member group of ‘protein serine histone kinases’. The biology of PSKH2 remains obscure, but it is most closely related to the Golgi‐associated canonical kinase PSKH1, which is a catalytically active member of the Ca2+‐CAM‐dependent protein kinases. Although PSKH1 and PSKH2 share many features in canonical catalytic residues, they also possess subtle differences when evaluated side‐by‐side most notably a validated Golgi‐targeting motif that is embedded in the N‐terminal region of PSKH1 that is conspicuously absent in PSKH2. This makes it unlikely, but theoretically still possible, that putative noncatalytic functions of PSKH2 might be performed by PSKH1 in organisms lacking PSKH2. In contrast, this pseudokinase‐specific region deletion in PSKH2 hints at distinct spatial and membrane identity‐determining roles that are distinct between each of the two proteins, although it is of interest that the putative SH3 binding motif found in PSKH1 is also conserved in PSKH2, as are putative sites of myristoylation and palmitoylation at the N‐terminal second Gly and third Cys positions, respectively. Dual acylation of PSKH1 has been shown to be important for Golgi targeting, whilst nonpalmitoylated PSKH1 remains in the ER. PSKH2 suggests interesting features that distinguish it from PSKH1, but which might also make it a useful model for studying evolutionary and functional constraints that underlie the conversion between canonical kinase PSKH1 and pseudokinase PSKH2. |
| 产品编号 | 产品名称 | 包装 |
| L30020 | pLenti-PSKH2-sgRNA | 5µg |
| — | 说明书 | 1份 |
-20℃保存，至少两年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA与质粒及其序列信息，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
慢病毒包装使用的包装质粒，可以订购碧云天的Lentivirus Packaging Vectors Set A (L00002)，包括pMDLg、Rev和VSV-g。
对于非目录产品的CRISPR基因敲除用的sgRNA表达质粒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
