pLenti-PKHD1L1-sgRNA (PKHD1L1基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。
碧云天同时提供基于CRISPR/Cas9技术的PKHD1L1基因敲除的质粒(L13425 pLenti-PKHD1L1-sgRNA)、慢病毒(L13426 PKHD1L1 Knockout Lentivirus)、HEK293T细胞(L13427 PKHD1L1 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L13428 PKHD1L1 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L13429 PKHD1L1 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
PKHD1L1基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | PKHD1L1 | 93035 | BC093096 | NM_177531 |
| About the gene | |
| Official Symbol | PKHD1L1 |
| Previous Symbol | - |
| Official Full Name | PKHD1 like 1 |
| Synonyms | PKHDL1 |
| Location | 8q23.1-q23.2 |
| Gene Type | protein_coding |
| Uniprot ID | Q86WI1 |
| Pathway/Library | Lung Cancer Growth Related Genes Library |
| Gene Summary | A novel stereociliary protein, Polycystic Kidney and Hepatic Disease 1-Like 1 (PKHD1L1), is a homolog of PKHD1, mutations of which cause autosomal–recessive polycystic kidney disease. Low levels of PKHD1L1 expression have been detected in primary immune cells. The predicted domain structure of PKHD1L1 suggests that the ectodomain may bind other proteins. The location of PKHD1L1 near the tips of OHC stereocilia suggests possible binding partners, but further studies are needed to evaluate possible interactions of PKHD1L1 with itself, with STRC and NPTN, with other TM components like CEACAM16 and TECTA, or with other proteins that form stereocilia surface specializations. Such interactions would help our understanding of PKHD1L1 function. PKHD1L1-deficient mice have progressive hearing loss, indicating that this protein is essential for normal hearing. The PKHD1L1 model will allow more extensive characterization of the function of PKHD1L1, and by extension the upper surface coat of stereocilia. PKHD1L1 is expressed by hair cells from P0 to P12, and is predominantly localized to the tips of OHC stereocilia, shows stronger labeling at the basal than apical turn of the cochlea at P0, is predominantly expressed in OHCs but less in IHCs, mice lacking PKHD1L1 show progressive hearing loss, and mice lacking PKHD1L1 lack the stereociliary coat at the tips of stereocilia. PKHD1L1 may be the major component of the upper surface coat of hair-cell stereocilia, and is essential for normal hearing especially at high frequencies. |
| 产品编号 | 产品名称 | 包装 |
| L13425 | pLenti-PKHD1L1-sgRNA | 5µg |
| — | 说明书 | 1份 |
-20℃保存，至少两年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA与质粒及其序列信息，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
慢病毒包装使用的包装质粒，可以订购碧云天的Lentivirus Packaging Vectors Set A (L00002)，包括pMDLg、Rev和VSV-g。
对于非目录产品的CRISPR基因敲除用的sgRNA表达质粒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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