分散酶II (≥0.5units/mg solid, Reagent grade)
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产品编号 ST2339-250mg
数量
¥ 639.00
产品包装:
50mg 250mg 1g
产品简介
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CAS Number Chemical Formula Molecular Weight Purity Grade
42613-33-2 ≥0.5units/mg solid Reagent grade

基本信息(General Information):

Name (Chinese) 分散酶II
Name (English) Dispase II
Specifications Reagent grade, ≥0.5units/mg, protease
Chemical Formula
Synonym (Chinese) 蛋白酶 来源于多粘芽孢杆菌
Synonym (English) Protease from Bacillus polymyxa
Beilstein Registry No. -
EINECS Number 255-914-4
MDL Number MFCD02253017
UNSPSC Code 12352204

产品描述(Description):

Analysis Note The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by dival
Application The enzyme from Beyotime has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the iso
Biochem/physiol Actions Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes.
Physical form lyophilized powder containing calcium acetate and milk sugar
Preparation Note A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C.
Unit Definition One unit will hydrolyze casein to produce color equivalent to 1.0 mumole (181 mug) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated.

性质(Properties):

activity ≥0.5 units/mg solid
solubility 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble
storage temp. 2-8°C

安全信息(Safety Information):

Hazard Pictogram Codes GHS07, GHS08
Signal Word Danger
Hazard Statements H315-H319-H334-H335
Precautionary Statements P261-P305 + P351 + P338-P342 + P311
Personal Protective Equipment -
Hazard Codes (Europe) Xn
Risk Codes (Europe) 36/37/38-42
Safety Codes (Europe) 22-24-26-36/37
RIDADR NONH for all modes of transport
WGK Germany 3
RTECS -
Flash Point (F) -
Flash Point (C) -
包装清单:
产品编号 产品名称 包装
ST2339-50mg 分散酶II (≥0.5units/mg solid, Reagent grade) 50mg
ST2339-250mg 分散酶II (≥0.5units/mg solid, Reagent grade) 250mg
ST2339-1g 分散酶II (≥0.5units/mg solid, Reagent grade) 1g
说明书 1份
保存条件:

4°C保存。

注意事项:

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