CAS Number | Chemical Formula | Molecular Weight | Purity | Grade |
42613-33-2 | ≥0.5units/mg solid | Reagent grade |
基本信息(General Information):
|
产品描述(Description):
Analysis Note | The enzyme can be initially dissolved in 50 mM Hepes/KOH pH 7.4, 150 mM NaCl at 10 mg/ml. Further dilutions should be made in the cell culture media most appropriate for the cells being dissociated. Dispase is a metalloproteinase and is activated by dival |
Application | The enzyme from Beyotime has been used in developing a protocol for ex vivo culture of mouse embryonic mammary buds. It has been used in the treatment of rat heart pieces during the isolation of mitochondria from rat heart. It has also been used for the iso |
Biochem/physiol Actions | Dispase II is a neutral protease that hydrolyzes the N-terminal peptide bonds of non-polar amino acid residues. It may be used for separating many tissues and cells grown in vitro. The enzyme is very gentle and does not damage cell membranes. |
Physical form | lyophilized powder containing calcium acetate and milk sugar |
Preparation Note | A 50 U/mL stock solution may be prepared by dissolving the powder in a buffer containing 10 mM NaAc (pH 7.5) and 5 mM CaAc. It should be stored at 4 °C. |
Unit Definition | One unit will hydrolyze casein to produce color equivalent to 1.0 mumole (181 mug) of tyrosine per min at pH 7.5 at 37°C (color by Folin-Ciocalteu reagent), unless otherwise indicated. |
性质(Properties):
activity | ≥0.5 units/mg solid |
solubility | 10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble |
storage temp. | 2-8°C |
安全信息(Safety Information):
Hazard Pictogram Codes | GHS07, GHS08 |
Signal Word | Danger |
Hazard Statements | H315-H319-H334-H335 |
Precautionary Statements | P261-P305 + P351 + P338-P342 + P311 |
Personal Protective Equipment | - |
Hazard Codes (Europe) | Xn |
Risk Codes (Europe) | 36/37/38-42 |
Safety Codes (Europe) | 22-24-26-36/37 |
RIDADR | NONH for all modes of transport |
WGK Germany | 3 |
RTECS | - |
Flash Point (F) | - |
Flash Point (C) | - |
产品编号 | 产品名称 | 包装 |
ST2339-50mg | 分散酶II (≥0.5units/mg solid, Reagent grade) | 50mg |
ST2339-250mg | 分散酶II (≥0.5units/mg solid, Reagent grade) | 250mg |
ST2339-1g | 分散酶II (≥0.5units/mg solid, Reagent grade) | 1g |
— | 说明书 | 1份 |
4°C保存。
注意事项:本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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