Recombinant Human TNF-α/TNFSF2, His-tag
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产品编号 P5322-10μg
数量
¥ 248.00
产品包装:
10μg 50μg 100μg 1mg
产品简介
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Species Gene ID Accession Source Length MW Tag
Human 7124 P01375 E. coli 164aa 18.3kDa His

About this protein
Name Recombinant Human TNF-α/TNFSF2, His-tag (Recombinant Human Tumor Necrosis Factor-α/TNFSF2, His-tag; rHuTNF-α/TNFSF2, His-tag);重组人肿瘤坏死因子α/TNFSF2,His标签
Synonyms Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Purity >97% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg in the presence of actinomycin D.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0.
Endotoxin Less than 1EU/μg of rHuTNF-α/TNFSF2, His-tag as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Cytokine
Background Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.
Amino Acid Sequence MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL

包装清单:

产品编号 产品名称 包装
P5322-10μg Recombinant Human TNF-α/TNFSF2, His-tag 10μg
P5322-50μg Recombinant Human TNF-α/TNFSF2, His-tag 50μg
P5322-100μg Recombinant Human TNF-α/TNFSF2, His-tag 100μg
P5322-1mg Recombinant Human TNF-α/TNFSF2, His-tag 1mg
- 说明书 1份

保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1.收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2.请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3.具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。
4.本产品的活性水经过严格测试,可以达到国外同类产品相一致的活性水平。使用本产品联合SM-164和Z-VAD-FMK诱导细胞坏死的效果参考图1。
图1.使用本产品联合SM-164和Z-VAD-FMK诱导NRK-52E (肾小管上皮细胞)细胞坏死的效果。图A为使用碧云天C0017 乳酸脱氢酶细胞毒性检测试剂盒检测1ng/ml本产品或国外同类竞争产品(Competitor)联合SM-164和Z-VAD-FMK诱导细胞坏死的结果;图B为使用碘化丙啶(PI)检测1mg/ml本产品或国外同类竞争产品联合SM-164和Z-VAD-FMK诱导细胞坏死的结果。Blank组添加了SM-164和Z-VAD-FMK(SZ),但没有添加TNF-α。实验结果表明,低至1ng/ml的本产品联合SM-164和Z-VAD-FMK即能诱导NRK-52E发生显著的坏死,本产品的活性和国外同类产品(Competitor)一致。注:SM-164和Z-VAD-FMK为联合TNFα诱导细胞坏死时使用,不随本产品提供,如有需要,请另行购买。

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