| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 7124 | P01375 | E. coli | 164aa | 18.3kDa | His |
| About this protein | |
| Name | Recombinant Human TNF-α/TNFSF2, His-tag (Recombinant Human Tumor Necrosis Factor-α/TNFSF2, His-tag; rHuTNF-α/TNFSF2, His-tag)；重组人肿瘤坏死因子α/TNFSF2，His标签 |
| Synonyms | Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.05ng/ml, corresponding to a specific activity of >2.0×107IU/mg in the presence of actinomycin D. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.0. |
| Endotoxin | Less than 1EU/μg of rHuTNF-α/TNFSF2, His-tag as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types. |
| Amino Acid Sequence | MHHHHHHVRS SSRTPSDKPV AHVVANPQAE GQLQWLNRRA NALLANGVEL RDNQLVVPSE GLYLIYSQVL FKGQGCPSTH VLLTHTISRI AVSYQTKVNL LSAIKSPCQR ETPEGAEAKP WYEPIYLGGV FQLEKGDRLS AEINRPDYLD FAESGQVYFG IIAL |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P5322-10μg | Recombinant Human TNF-α/TNFSF2, His-tag | 10μg |
| P5322-50μg | Recombinant Human TNF-α/TNFSF2, His-tag | 50μg |
| P5322-100μg | Recombinant Human TNF-α/TNFSF2, His-tag | 100μg |
| P5322-1mg | Recombinant Human TNF-α/TNFSF2, His-tag | 250μg×4 |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1.收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2.请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3.具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
4.本产品的活性水经过严格测试，可以达到国外同类产品相一致的活性水平。使用本产品联合SM-164和Z-VAD-FMK诱导细胞坏死的效果参考图1。
图1.使用本产品联合SM-164和Z-VAD-FMK诱导NRK-52E (肾小管上皮细胞)细胞坏死的效果。图A为使用碧云天C0017 乳酸脱氢酶细胞毒性检测试剂盒检测1ng/ml本产品或国外同类竞争产品(Competitor)联合SM-164和Z-VAD-FMK诱导细胞坏死的结果；图B为使用碘化丙啶(PI)检测1mg/ml本产品或国外同类竞争产品联合SM-164和Z-VAD-FMK诱导细胞坏死的结果。Blank组添加了SM-164和Z-VAD-FMK(SZ)，但没有添加TNF-α。实验结果表明，低至1ng/ml的本产品联合SM-164和Z-VAD-FMK即能诱导NRK-52E发生显著的坏死，本产品的活性和国外同类产品(Competitor)一致。注：SM-164和Z-VAD-FMK为联合TNFα诱导细胞坏死时使用，不随本产品提供，如有需要，请另行购买。
