Recombinant Human TIM4 (His-Tag&Avi-Tag)
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产品编号 P4305-50μg
数量
¥ 1878.00
产品包装:
10μg 50μg 200μg 1mg
产品简介
使用说明
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Species Gene ID Accession Source Length MW Tag
Human 91937 Q96H15 HEK293 342aa 38kDa C-His-Tag & Avi-Tag

蛋白信息(About this protein)
名称(Name) Recombinant Human TIM4 (His-Tag&Avi-Tag); 重组His&Avi标签人TIM4; His/Avi-hTIM4
别名(Synonyms) T-cell immunoglobulin and mucin domain containing 4, T-cell membrane protein 4, T-cell immunoglobulin mucin receptor 4, TIM-4, TIMD4, SMUCKLER, His/Avi-hTIM4
产品简介
(Background)
Beyotime's Recombinant Human TIM4 (His-Tag&Avi-Tag) was expressed in HEK293 and purified, which contain the extracellular domain (25-314aa) of human TIM4 with His-Tag and Avi-Tag fusion at the C-terminus.
TIMs are type I transmembrane proteins, with the N terminus containing the variable Ig-like (IgV) domain extending from the plasma membrane and the C-terminal tail largely mediating intracellular signaling oriented toward the cytosol. The Tim gene family consists of eight members (Tim-1-8) on mouse chromosome 11B1.1 and three members (Tim-1, Tim-3, and Tim-4) on human chromosome 5q33.2. TIM1, TIM2, and TIM3 were all found to be expressed by T cells and involved in the regulation of Th cells. TIM4 is different from other TIM molecules in that it is expressed exclusively on antigen-presenting cells (APCs), particularly on mature myeloid-derived DCs and macrophages but not by lymphocytes.
Mature human TIM4 consists of a 290aa extracellular domain (ECD), a 21aa transmembrane segment and a 43aa cytoplasmic tail. TIM4 is involved in immune regulation such as to initiate skewed Th2 polarization and to mediate phagocytosis of apoptotic cells. TIM4 also binds specifically to TIM1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma. TIM4 is also found in tumor tissue such as histiocytic and dendritic cell neoplasms, which consistently express TIM4. It has been suggested that these molecules can be new markers of neoplasms derived from histiocytic and dendritic cells. TIM4 contains IgV domain with integrin-binding site as well as a unique metal-ion-dependent ligand binding site for phosphatidylserine (PS). Binding of TIM4 to PS displays the highest affinity and depends on Ca2+. This feature allows to efficiently capture SEVs(Small extracellular vesicles)using TIM4-beads and then elute intact SEVs from the TIM4-beads [1-2].
产品用途
(Applications)
Ligand for HAVCR1/TIMD1. Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Recombinant Human TIM4 (His-Tag&Avi-Tag) can be used to detect or isolate apoptotic cells or exosomes.
外观(Physical appearance) Liquid
内毒素(Endotoxin) <0.1EU/μg by LAL method
浓度(Concentration) 0.5mg/ml
纯度(Purity) >95% by SDS-PAGE
储存液(Storage buffer) TBS pH7.6  0.1

本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图1。Recombinant Human TIM4 (His-Tag&Avi-Tag)蛋白理论分子量为38kDa,因含有N-糖基化修饰,蛋白实际分子量约为65kDa。

图1. 碧云天Recombinant Human TIM4 (His-Tag&Avi-Tag) (P4305)的SDS-PAGE电泳检测图。经BeyoGel™ Plus PAGE预制胶(Tris-Gly, 15%, 10孔) (P0461)电泳,Marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) (P0071/P0072),并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际检测结果可能会因样品和检测条件等的不同而存在差异,图中数据仅供参考。

包装清单:
产品编号 产品名称 包装
P4305-10μg Recombinant Human TIM4 (His-Tag&Avi-Tag) 10μg
P4305-50μg Recombinant Human TIM4 (His-Tag&Avi-Tag) 50μg
P4305-200μg Recombinant Human TIM4 (His-Tag&Avi-Tag) 200μg
P4305-1mg Recombinant Human TIM4 (His-Tag&Avi-Tag) 200μg×5
说明书 1份
保存条件:

-20ºC保存,一年有效。-80ºC保存,可以保存更长时间。

注意事项:

由于蛋白每次冻融均会引起部分失活,所以首次配制成相应浓度储存液后,须分装后-20ºC或更低温度冻存,尽量避免反复冻融。

本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。

为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 本产品C端带有Avi标签,Avi标签是由15个氨基酸(GLNDIFEAQKIEWHE)组成的短肽标签,在ATP和生物素存在的条件下,BirA在Avi标签的赖氨酸残基上连接一个生物素,从而实现目的蛋白的生物素标记[3]。推荐使用碧云天Avi标签蛋白生物素标记试剂盒(BirA法) (P0630)进行生物素标记。
2. 收到产品后请立即按照说明书推荐的条件保存。在打开管盖前,请适当离心,使附着在管盖或管壁上的蛋白聚集于管底。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。
参考文献:
1. Xu L, Qi J, Zhao P, Liang X, Ju Y, et al. J Leukoc Biol. 2010. 88(2):329-36.
2. Yoshida T, Hanayama R. Methods Mol Biol. 2022. 2466:23-36.
3. Cull MG, Schatz PJ. Methods Enzymol. 2000. 326:430-40.
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P0371 生物素定量检测试剂盒(显色法) 100次/500次
P0373 生物素定量检测试剂盒(荧光法) 100次/500次
P0630 Avi标签蛋白生物素标记试剂盒(BirA法) 20次/100次/500次
P4301 Recombinant Biotinylated Human TIM4 (Avi-Tag) 10μg/50μg/200μg/1mg
P4303 Recombinant Human TIM4 (His-Tag) 10μg/50μg/200μg/1mg
P4305 Recombinant Human TIM4 (His-Tag&Avi-Tag) 10μg/50μg/200μg/1mg
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