| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 91937 | Q96H15 | HEK293 | 342aa | 38kDa | C-His-Tag & Avi-Tag |
| 蛋白信息(About this protein) | |
| 名称(Name) | Recombinant Human TIM4 (His-Tag&Avi-Tag); 重组His&Avi标签人TIM4; His/Avi-hTIM4 |
| 别名(Synonyms) | T-cell immunoglobulin and mucin domain containing 4, T-cell membrane protein 4, T-cell immunoglobulin mucin receptor 4, TIM-4, TIMD4, SMUCKLER, His/Avi-hTIM4 |
| 产品简介 (Background) |
Beyotime's Recombinant Human TIM4 (His-Tag&Avi-Tag) was expressed in HEK293 and purified, which contain the extracellular domain (25-314aa) of human TIM4 with His-Tag and Avi-Tag fusion at the C-terminus. TIMs are type I transmembrane proteins, with the N terminus containing the variable Ig-like (IgV) domain extending from the plasma membrane and the C-terminal tail largely mediating intracellular signaling oriented toward the cytosol. The Tim gene family consists of eight members (Tim-1-8) on mouse chromosome 11B1.1 and three members (Tim-1, Tim-3, and Tim-4) on human chromosome 5q33.2. TIM1, TIM2, and TIM3 were all found to be expressed by T cells and involved in the regulation of Th cells. TIM4 is different from other TIM molecules in that it is expressed exclusively on antigen-presenting cells (APCs), particularly on mature myeloid-derived DCs and macrophages but not by lymphocytes. Mature human TIM4 consists of a 290aa extracellular domain (ECD), a 21aa transmembrane segment and a 43aa cytoplasmic tail. TIM4 is involved in immune regulation such as to initiate skewed Th2 polarization and to mediate phagocytosis of apoptotic cells. TIM4 also binds specifically to TIM1 which is also the cellular receptor for hepatitis A virus and has been implicated in the development of asthma. TIM4 is also found in tumor tissue such as histiocytic and dendritic cell neoplasms, which consistently express TIM4. It has been suggested that these molecules can be new markers of neoplasms derived from histiocytic and dendritic cells. TIM4 contains IgV domain with integrin-binding site as well as a unique metal-ion-dependent ligand binding site for phosphatidylserine (PS). Binding of TIM4 to PS displays the highest affinity and depends on Ca2+. This feature allows to efficiently capture SEVs（Small extracellular vesicles）using TIM4-beads and then elute intact SEVs from the TIM4-beads [1-2]. |
| 产品用途 (Applications) |
Ligand for HAVCR1/TIMD1. Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Recombinant Human TIM4 (His-Tag&Avi-Tag) can be used to detect or isolate apoptotic cells or exosomes. |
| 外观(Physical appearance) | Liquid |
| 内毒素（Endotoxin) | <0.1EU/μg by LAL method |
| 浓度(Concentration) | 0.5mg/ml |
| 纯度(Purity) | >95% by SDS-PAGE |
| 储存液(Storage buffer) | TBS pH7.6 0.1 |
本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图1。Recombinant Human TIM4 (His-Tag&Avi-Tag)蛋白理论分子量为38kDa，因含有N-糖基化修饰，蛋白实际分子量约为65kDa。
图1. 碧云天Recombinant Human TIM4 (His-Tag&Avi-Tag) (P4305)的SDS-PAGE电泳检测图。经BeyoGel™ Plus PAGE预制胶(Tris-Gly, 15%, 10孔) (P0461)电泳，Marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) (P0071/P0072)，并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
包装清单：| 产品编号 | 产品名称 | 包装 |
| P4305-10μg | Recombinant Human TIM4 (His-Tag&Avi-Tag) | 10μg |
| P4305-50μg | Recombinant Human TIM4 (His-Tag&Avi-Tag) | 50μg |
| P4305-200μg | Recombinant Human TIM4 (His-Tag&Avi-Tag) | 200μg |
| P4305-1mg | Recombinant Human TIM4 (His-Tag&Avi-Tag) | 200μg×5 |
| － | 说明书 | 1份 |
-20ºC保存，一年有效。-80ºC保存，可以保存更长时间。
注意事项：由于蛋白每次冻融均会引起部分失活，所以首次配制成相应浓度储存液后，须分装后-20ºC或更低温度冻存，尽量避免反复冻融。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| P0371 | 生物素定量检测试剂盒(显色法) | 100次/500次 |
| P0373 | 生物素定量检测试剂盒(荧光法) | 100次/500次 |
| P0630 | Avi标签蛋白生物素标记试剂盒(BirA法) | 20次/100次/500次 |
| P4301 | Recombinant Biotinylated Human TIM4 (Avi-Tag) | 10μg/50μg/200μg/1mg |
| P4303 | Recombinant Human TIM4 (His-Tag) | 10μg/50μg/200μg/1mg |
| P4305 | Recombinant Human TIM4 (His-Tag&Avi-Tag) | 10μg/50μg/200μg/1mg |
