HUNK Knockout Lentivirus(HUNK基因敲除慢病毒)是一种感染动物细胞后可以同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除目的基因，并且本慢病毒中sgRNA的有效性已经通过T7EI法的验证。
本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 可同时表达sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的关键图谱信息。
用于包装本慢病毒的质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本慢病毒用于实验时，建议同时选购无任何靶向的对照慢病毒Control Knockout Lentivirus (L00015)或靶向GFP的对照慢病毒GFP Knockout Lentivirus (L00017)。
碧云天同时提供基于CRISPR/Cas9技术的HUNK基因敲除的质粒(L27815 pLenti-HUNK-sgRNA)、慢病毒(L27816 HUNK Knockout Lentivirus)、HEK293T细胞(L27817 HUNK Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L27818 HUNK Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L27819 HUNK Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
HUNK基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | HUNK | 30811 | - | NM_014586 |
| About the gene | |
| Official Symbol | HUNK |
| Previous Symbol | - |
| Official Full Name | hormonally up-regulated Neu-associated kinase |
| Synonyms | - |
| Location | 21q22.11 |
| Gene Type | protein_coding |
| Uniprot ID | P57058 |
| Pathway/Library | others |
| Gene Summary | Hormonally Up-regulated Neu-associated Kinase (HUNK) is a serine/threonine kinase that is a member of the AMP-activated protein kinase (AMPK) family, and was reported to be up-regulated in aggressive subsets of human cancers including breast, ovarian, and colon cancers. Experimental studies indicate a relationship between HUNK and breast cancer progression. Wertheim et al. showed that Hunk wild-type (Hunk+/+) mice had significantly increased lung metastases compared with Hunk-knockout (Hunk−/−) mice when bred into an MMTV-myc background. In addition, tumors derived from Hunk−/− cells retrovirally transduced with a wild-type version of HUNK (HUNK WT) had significantly increased lung metastases compared with those transduced with either a kinase dead version of HUNK (K91M) or a control vector. These data strongly suggest that HUNK promotes breast cancer metastasis, and that this process is dependent on HUNK kinase activity. However, another study provided evidence that HUNK suppresses basal breast cancer metastasis. Quintela-Fandino et al. found that when HUNK was exogenously introduced into basal breast cancer cells, cell motility and tumor metastases were decreased. HUNK directly phosphorylates EGFR in its juxtamembrane domain at threonine 654 (T654), resulting in receptor stabilization and signaling. This effect corresponds to an increase in epithelial to mesenchymal (EMT), cell migration, and invasion in vitro. HUNK stabilizes EGFR expression in primary tumors, which correlates with increased levels of phosphorylation of EGFR at T654, resulting in an increase in lung metastasis in vivo. |
| 产品编号 | 产品名称 | 包装 |
| L27816 | HUNK Knockout Lentivirus | 108 TU |
| — | 说明书 | 1份 |
-80℃保存，至少一年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本慢病毒，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
对于非目录产品的CRISPR基因敲除用的慢病毒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| L00015 | Control Knockout Lentivirus | 108 TU |
| L00017 | GFP Knockout Lentivirus | 108 TU |
| C0222 | 青霉素-链霉素溶液(100X) | 100ml |
| C0351-1ml | Polybrene (Hexadimethrine Bromide) | 1ml |
| C0351-50mg | Polybrene (Hexadimethrine Bromide) | 50mg |
| D0508S/M | 基因组编辑突变检测试剂盒 | 25/100次 |
| D7080S/M/L | T7 Endonuclease I (CRISPR等基因突变鉴定用) | 250/1250/5000U |
| ST551-10mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×1ml |
| ST551-50mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×5ml |
| ST551-250mg | Puromycin Dihydrochloride (嘌呤霉素) | 250mg |
| ST1380-500mg | Polybrene (≥94%, Reagent grade) | 500mg |
| ST1380-2g | Polybrene (≥94%, Reagent grade) | 2g |
| ST1380-10g | Polybrene (≥94%, Reagent grade) | 10g |
