生物素标记山羊抗兔IgG(H+L)
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产品编号 A0277
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¥ 350.00
产品包装:
1ml
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来源 用途 WB/ELISA IHC/IC IF/F
Goat WB, ELISA, IHC, IC, IF, F 1:1000-3000 1:50-500 1:25-250

WB, Western blot; IHC,Immunohistochemistry; IC,Immunocytochemistry; IF,Immunofluorescence; F,Flow cytometry。
本生物素标记山羊抗兔IgG(H+L)(Biotin-labeled Goat Anti-Rabbit IgG(H+L))为进口分装,用于Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析的高纯度高特异性二抗。
生物素标记后可以采用辣根过氧化物酶标记的Streptavidin或碱性磷酸酯酶标记的Streptavidin进行后续的检测。随后用辣根过氧化物酶或碱性磷酸酯酶对应的检测体系进行显色反应或发光反应等。
本抗体为用纯化的兔IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG、小鼠IgG和大鼠IgG吸附纯化的优质二抗。对人、小鼠和大鼠的血清蛋白几乎没有结合能力(Minimal Cross-reaction to Human, Mouse and Rat Serum Proteins)。
本生物素标记山羊抗兔IgG(H+L)用于Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析的推荐稀释比例参考上表,实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。
本抗体如果用于常规的Western检测,以每次检测需10毫升1:1000稀释的二抗计,可以检测100次。
包装清单:

产品编号 产品名称 包装
A0277 生物素标记山羊抗兔IgG (H+L) 1ml
说明书 1份

保存条件:
-20℃保存,一年有效。
注意事项:
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. Western、ELISA、免疫组化、免疫细胞化学、免疫荧光和流式细胞仪分析请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
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