辣根过氧化物酶标记山羊抗小鼠IgG(H+L)
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产品编号 A0216
数量
¥ 226.00
产品包装:
1ml
产品简介
使用说明
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来源 用途 WB ELISA IHC
Goat WB, ELISA, IHC 1:1000 1:250 1:50

WB, Western blot; ELISA, Enzyme-linked Immunosorbent Assay; IHC, Immunohistochemistry.
本辣根过氧化物酶标记山羊抗小鼠IgG(H+L)(HRP-labeled Goat Anti-Mouse IgG(H+L))为进口分装,用于Western、ELISA和IHC等的二抗。HRP,即horseradish peroxidase,中文名为辣根过氧化物酶,可以在Western检测时催化ECL类试剂(如ECL、BeyoECL等)产生化学发光,可以在ELISA时催化TMB产生蓝色,也可以在IHC或Western blot检测时催化DAB产生棕色沉淀。
本辣根过氧化物酶标记山羊抗小鼠IgG(H+L)用于WB、ELISA和IHC的推荐稀释比例参考上表,实际实验操作过程中需根据抗原和抗体的具体情况适当调节二抗的稀释比例。对于WB,推荐的稀释范围为1:500-2000。
本抗体为用纯化的小鼠IgG免疫山羊,然后用亲和纯化柱对获得的抗血清进行纯化,并经过人IgG吸附纯化的优质二抗。对人IgG几乎没有结合能力。
本抗体如果用于常规的Western检测,以每次检测需10毫升1:1000稀释的二抗计,至少可以检测100次。
包装清单:

产品编号 产品名称 包装
A0216 辣根过氧化物酶标记山羊抗小鼠IgG (H+L) 1ml
说明书 1份

保存条件:
-20℃保存,一年有效。4℃保存,半年有效。
注意事项:
叠氮钠会抑制辣根过氧化物酶的活性,在含有辣根过氧化物酶的二抗中不可加入叠氮钠。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. Western、ELISA或IHC请参考相关实验步骤进行。起始稀释浓度按照产品简介中推荐的稀释比例进行稀释。
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