| Species | Gene ID | Accession | Source | Length | Calculated MW | Tag |
| - | - | - | E. coli | 365aa | ~40kDa | － |
| About this protein | |
| Name | Recombinant Protein L (rProtein L; Protein L)；重组蛋白L |
| Synonyms | N/A |
| Purity | >98% by SDS-PAGE and SEC-HPLC analyses. |
| Biological Activity | The recombinant Protein L is a genetically engineered protein containing 5 IgG-binding regions of protein L. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein L to ensure the maximum specific IgG binding. The recombinant Protein L is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein L binds to human, mouse, rat and pig IgG. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from additive free solution. |
| Endotoxin | Less than 0.1EU/μg of Protein L as determined by LAL method. |
| Reconstitution | Dissolve in distilled water or saline. |
| Category | Protein L |
| Background | Recombinant Protein L contains 5 kappa-binding domains. Protein L was first isolated from the surface of bacterial species Peptostreptoccus magnus [1]. Unlike Protein A and Protein G, which bind to the Fc region of immunoglobulins (antibodies), Protein L binds antibodies through kappa light chain interactions without interfering with an antibody’s antigen-binding site. This gives Protein L the ability to bind a wider range of Ig classes and subclasses (IgG, IgM, IgA, IgE and IgD) than Protein A or Protein G. Single chain variable fragments (scFv) and Fab fragments also bind to Protein L. Protein L binds kappa I, III, and IV in human and kappa I in mouse [2]. |
| Amino Acid Sequence | KEETPETPET DSEEEVTIKA NLIFANGSTQ TAEFKGTFEK ATSEAYAYAD TLKKDNGEYT VDVADKGYTL NIKFAGKEKT PEEPKEEVTI KANLIYADGK TQTAEFKGTF EEATAEAYRY ADALKKDNGE YTVDVADKGY TLNIKFAGKE KTPEEPKEEV TIKANLIYAD GKTQTAEFKG TFEEATAEAY RYADLLAKEN GKYTVDVADK GYTLNIKFAG KEKTPEEPKE EVTIKANLIY ADGKTQTAEF KGTFAEATAE AYRYADLLAK ENGKYTADLE DGGYTINIRF AGKKVDEKPE EKEQVTIKEN IYFEDGTVQT ATFKGTFAEA TAEAYRYADL LSKEHGKYTA DLEDGGYTIN IRFAG |
| 产品编号 | 产品名称 | 包装 |
| P5076-10mg | Recombinant Protein L | 10mg |
| P5076-100mg | Recombinant Protein L | 10mg×10 |
| P5076-1g | Recombinant Protein L | 1g |
| — | 说明书 | 1份 |
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项：由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
