| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 4893 | P01111 | E.coli | 196aa | ~22kDa | N-Flag Tag |
| 蛋白信息(About this protein) | |
| 名称(Name) | Recombinant Human NRas; 重组人NRas蛋白 |
| 别名(Synonyms) | Recombinant Human N-Ras, 重组人N-Ras蛋白, NRas Proto-Oncogene; Transforming Protein N-Ras; Neuroblastoma Ras Viral (V-Ras) Oncogene Homolog, Neuroblastoma Ras Viral oncogene Homolog; proto-oncogene GTPase; ALPS4; AV095280; N-Ras; HRAS1; NS6 |
| 产品简介(Background) | Beyotime’s recombinant human NRas (rhNRas) was expressed in E.coli and purified, which contain the mature form NRas (2-186aa) with Flag tag (DYKDDDDK) at the N-terminus. Ras (NRas, HRas and KRas) is the most frequently mutated gene family in cancers，they differ significantly only in the C-terminal 40 amino acids. These Ras genes have GTP/GDP binding and GTPase activity, and their normal function may be as G-like regulatory proteins involved in the normal control of cell growth. Ras directly interacts with and activates several downstream effector pathways including the mitogen-activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI3K) pathways. Mutations in Ras gene disrupt the guanine exchange cycle, typically by becoming GAP-independent and ‘locking’ Ras in the active, GTP-bound state, thereby activating downstream signaling pathways resulting in tumor cell growth. NRas oncogene encoding a membrane protein that shuttles between the Golgi apparatus and the plasma membrane. This shuttling is regulated through palmitoylation and depalmitoylation by the ZDHHC9-GOLGA7 complex. Mutations which change amino acid residues 12, 13 or 61 activate the potential of NRas to transform cultured cells and are implicated in a variety of human tumors, such as somatic rectal cancer, follicular thyroid cancer, autoimmune lymphoproliferative syndrome, Noonan syndrome, and juvenile myelomonocytic leukemia [1]. |
| 产品用途(Applications) | Recombinant Human NRas is useful in studying NRas interacting proteins, effectors, GAPs (GTPase-activating proteins) and GEFs (Guanine nucleotide-exchange factors). It can also be used as positive control in Western blots. |
| 外观(Physical appearance) | Liquid |
| 活性(Biological activity) | The specific activity of NRas was 4.3 nmol/min/mg in GTPase activity assay. |
| 浓度(Concentration) | 1mg/ml |
| 纯度(Purity) | ≥ 95% by SDS-PAGE |
| 储存液(Storage buffer) | 20mM HEPES (pH7.4), 200mM NaCl, 1mM EDTA, 2mM DTT, 5% glycerol |
小GTP酶(Small GTPase)，也称Small G-proteins、Ras superfamily，是调节真核细胞信号转导、细胞增殖、细胞骨架重组和细胞内膜转运等过程的分子开关，小GTP酶通过结合和水解GTP，在“激活”和“静息”状态之间循环：在外界信号的刺激下，鸟苷酸交换因子(Guanine nucleotide-exchange factors, GEFs)辅助小GTP酶将结合的GDP置换为GTP，小GTP酶结合GTP进入激活状态(Active state)；激活状态的小GTP酶与下游效应蛋白(Effector protein)相互作用，从而刺激细胞发生相应的响应；GTP酶激活蛋白(GTPase-activating proteins, GAPs)催化小GTP酶结合的GTP水解为GDP，并释放自由磷酸盐(Free phosphate, Pi)，此时小GTP酶结合GDP进入静息状态(Inactive state)，鸟嘌呤核苷酸解离抑制蛋白(Guanine nucleotide dissociation inhibitors, GDIs)抑制小GTP酶释放GDP，直到GEFs受到刺激信号再次开启新一轮的循环(图1) [2-3]。
图1.小GTP酶在“激活”和“静息”状态之间循环的原理图。
小GTP酶在人类中已发现超过150个家族成员，在果蝇(Drosophila)、秀丽隐杆线虫(C. elegans)、酿酒酵母(S. cerevisiae)、粟酒裂殖酵母(S. pombe)、黏菌(Dictyostelium)和植物中也都发现了保守的同源物。小GTP酶根据结构和功能被分为5个家族分支：Ras家族、Rho家族、Ran家族、Rab家族和Arf家族。Ras家族本身又被分为6个亚家族：Ras亚家族、Ral亚家族、Rit亚家族、Rap亚家族、Rheb亚家族和Rad亚家族[4]。
Ras家族(Ras homologous GTPases)是调节肌动蛋白重组的关键因子，因此在肌动蛋白细胞骨架完整性、细胞增殖、细胞分化、细胞粘附、细胞凋亡和细胞迁移等细胞过程中发挥着重要作用。目前有16个成员被发现，被分为6个亚家族(见下表)，其中NRas、HRas和KRas研究的最为广泛[5]。
| Subfamily | Ras | Ral | Rit | Rap | Rheb | Rad |
| Subfamily members | HRas NRas KRas4A KRas4B |
RalA RalB |
Rit1 Rit2 |
Rap1A Rap1B Rap2A Rap2B Rap2C |
Rheb RhebL1 |
RRad |
本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图2。
图2.碧云天Recombinant Human NRas (P2401)的SDS-PAGE电泳检测图。本蛋白经BeyoGel™ Plus PAGE预制胶(Tris-Gly, 4-15%, 10孔) (P0465)电泳，Marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) (P0071/P0072)，并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
包装清单：| 产品编号 | 产品名称 | 包装 |
| P2401-10μg | Recombinant Human NRas (Flag-Tag) | 10μg |
| P2401-100μg | Recombinant Human NRas (Flag-Tag) | 100μg |
| P2401-1mg | Recombinant Human NRas (Flag-Tag) | 1mg |
| — | 说明书 | 1份 |
-20ºC保存，一年有效。-80ºC保存，可以保存更长时间。
注意事项：由于蛋白每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后，须分装后-20ºC或更低温度冻存，尽量避免反复冻融。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| P2061-1ml | Rhotekin-RBD Agarose (活性Rho结合琼脂糖凝胶) | 1ml |
| P2063-10μg | Recombinant Human RhoA (Flag-Tag) | 10μg |
| P2063-100μg | Recombinant Human RhoA (Flag-Tag) | 100μg |
| P2063-1mg | Recombinant Human RhoA (Flag-Tag) | 1mg |
| P2065S | Active Rho Pull-down and Detection Kit | 50次 |
| P2065M | Active Rho Pull-down and Detection Kit | 250次 |
| P2401-10μg | Recombinant Human NRas (Flag-Tag) | 10μg |
| P2401-100μg | Recombinant Human NRas (Flag-Tag) | 100μg |
| P2401-1mg | Recombinant Human NRas (Flag-Tag) | 1mg |
| P2403-10μg | Recombinant Human HRas (His-Tag) | 10μg |
| P2403-100μg | Recombinant Human HRas (His-Tag) | 100μg |
| P2403-1mg | Recombinant Human HRas (His-Tag) | 1mg |
| P2405-10μg | Recombinant Human KRas4A (His-Tag) | 10μg |
| P2405-100μg | Recombinant Human KRas4A (His-Tag) | 100μg |
| P2405-1mg | Recombinant Human KRas4A (His-Tag) | 1mg |
| P2407-10μg | Recombinant Human KRas4B (His-Tag) | 10μg |
| P2407-100μg | Recombinant Human KRas4B (His-Tag) | 100μg |
| P2407-1mg | Recombinant Human KRas4B (His-Tag) | 1mg |
| P2409-10μg | Recombinant Human KRas4B (G12C, His-Tag) | 10μg |
| P2409-100μg | Recombinant Human KRas4B (G12C, His-Tag) | 100μg |
| P2409-1mg | Recombinant Human KRas4B (G12C, His-Tag) | 1mg |
| P2411-10μg | Recombinant Human KRas4B (G12D, His-Tag) | 10μg |
| P2411-100μg | Recombinant Human KRas4B (G12D, His-Tag) | 100μg |
| P2411-1mg | Recombinant Human KRas4B (G12D, His-Tag) | 1mg |
| P2413-10μg | Recombinant Human KRas4B (G12V, His-Tag) | 10μg |
| P2413-100μg | Recombinant Human KRas4B (G12V, His-Tag) | 100μg |
| P2413-1mg | Recombinant Human KRas4B (G12V, His-Tag) | 1mg |
| P2415-10μg | Recombinant Human KRas4B (G13D, His-Tag) | 10μg |
| P2415-100μg | Recombinant Human KRas4B (G13D, His-Tag) | 100μg |
| P2415-1mg | Recombinant Human KRas4B (G13D, His-Tag) | 1mg |
| P2417-10μg | Recombinant Human KRas4B (G13S, His-Tag) | 10μg |
| P2417-100μg | Recombinant Human KRas4B (G13S, His-Tag) | 100μg |
| P2417-1mg | Recombinant Human KRas4B (G13S, His-Tag) | 1mg |
| P2419-10μg | Recombinant Human KRas4B (Q61H, His-Tag) | 10μg |
| P2419-100μg | Recombinant Human KRas4B (Q61H, His-Tag) | 100μg |
| P2419-1mg | Recombinant Human KRas4B (Q61H, His-Tag) | 1mg |
| AF0273 | Ras Rabbit Polyclonal Antibody | 50μl |
| AF1168 | Ras Rabbit Monoclonal Antibody | 50μl |
| AF7131 | HRas Rabbit Polyclonal Antibody | 50μl |
| AF7347 | KRAS Rabbit Polyclonal Antibody | 50μl |
| AG2391 | KRAS Rabbit Monoclonal Antibody | 50μl |
| AG2394 | KRAS Mouse Monoclonal Antibody | 50μl |
