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GAPDH抗体
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产品编号: AG019
产品包装: >40次
产品价格: 399.00元 |
![](reagent-images/AG019-145x110.jpg) |
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产品简介
产品简介:
产品编号 |
产品名称 |
产品包装 |
产品价格 |
AG019 |
GAPDH抗体 |
>40次 |
399.00元 |
来源 |
用途 |
可检测样品 |
抗体类型 |
GAPDH分子量 |
Mouse |
WB, IF, IHC, ELISA |
Mam, C, Fi, Fr |
IgG1 |
~36kD |
WB, Western blot; IF, Immunofluorescence; IHC, Immunohistochemistry.
Mam, mammalian, including human, mouse, rat, pig, rabbit, dog, cat; C, chicken; Fi,
fish; Fr, frog. 不能用于检测bovine和yeast样品。
本GAPDH抗体(GAPDH antibody,也称为G3PDH antibody)为进口分装,用从兔肌肉纯化获得的GAPDH
作为抗原制备而成的抗GAPDH小鼠单克隆抗体。克隆号为6C5。
GAPDH(Glyceraldehyde-3-phosphate dehydrogenase)是糖酵解(glycolysis)过程中的关键酶之一。GAPDH由4个分子量约为36kD的亚基组成,总的分子量为146kD。在SDS-PAGE凝胶电泳时解聚为单体,显示的分子量约为36kD。哺乳动物的GAPDH除了在糖酵解过程中起作用外,还和membrane fusioin,microtubule bundling,RNA出核运输,DNA复制和DNA修复等有关,并和前列腺癌、神经细胞凋亡、神经退行性疾病等相关。
GAPDH几乎在所有组织和细胞中组成性高表达。只有缺氧和糖尿病等情况可以在某些细胞中上调
GAPDH的蛋白水平。
GAPDH在各种组织和细胞中都组成性高表达,因此被广泛用于Western时上样量是否一致的参照
(loading control)。
配套提供了Western一抗稀释液,可以用于Western检测时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB |
IF |
IHC |
ELISA |
1:500-1000 |
1:400 |
1:400 |
1:1000-5000 |
本抗体如果用于常规的Western检测,至少可以检测40次。
包装清单:
产品编号 |
产品名称 |
包装 |
AG019-1 |
GAPDH抗体 |
40μl |
AG019-2 |
Western一抗稀释液 |
40ml |
- |
说明书 |
1份 |
保存条件:
GAPDH抗体-20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
在Western实验后,请注意回收稀释的抗体。回收的抗体在进行Western实验时至少可以重复使用10
次。稀释后的抗体,包括已经使用过的稀释抗体,4℃保存。
回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混
浊现象,可以10000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明
产品图片
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