Proteinase K (20mg/ml)
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产品编号 ST532
数量
¥ 61.00
产品包装:
0.2ml
产品简介
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      ST532现已更改为ST533-0.2ml
      Proteinase K,中文名为蛋白酶K。不含DNase,不含RNase,进口分装,可直接使用。可以用于消化各种蛋白。用于各种常见的分子生物学、细胞生物学等相关实验,例如基因组DNA抽提、酶的消化去除等。 
      酶活力,>30U/mg。在37℃,以血红蛋白为底物,1分钟内可以产生相当于1微摩尔酪氨酸Folin阳性的氨基酸或多肽的蛋白酶K的量,定义为一个单位蛋白酶K酶活力。 
      在很宽的pH范围内有效,有效的pH范围为pH4.0-12.5,最佳pH范围为pH7.5-8.0。 
      蛋白酶K的最佳反应温度为65℃,但在65℃或更高的温度蛋白酶K自身的也非常迅速地降解。很多时候反应温度选择50-55℃。 
      在0.2-1%SDS或约10mM尿素存在的情况下,蛋白酶K显示更高的酶活性。蛋白酶K的常用工作浓度为0.05-1mg/ml,根据所用缓冲液是否含有SDS、尿素、pH是否适合、温度是否适合等因素确定具体的工作浓度。 
      常用浓度的EDTA、Triton X-100、Tween 20、Sarkosyl、盐酸胍对蛋白酶K的活力影响不大。
      如果需要把20mg/ml的蛋白酶K稀释成一定浓度,推荐用P0106 免疫染色洗涤液或10mM Tris-HCl pH7.4-7.8进行稀释。
包装清单:

产品编号 产品名称 包装
ST532 Proteinase K (20mg/ml) 0.2ml
说明书 
1份

保存条件:
      -20℃保存。
注意事项:
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
      为了您的安全和健康,请穿实验服并戴一次性手套操作。


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