中文名 | 东莨菪内酯 |
英文名 | Scopoletin |
中文别名 | 莨菪亭;东莨菪亭 |
英文别名 | Chysatroic acid;Escoopoletin;Gelseminic acid;Scopoletol;6-Methoxy-7-hydroxycoumarin |
来源 | 丁公藤Erycibe obtusifolia Benth.;白花蛇舌草Hedyotis diffusa Willd;东莨菪Scopolia japonica Maxin.; |
化合物类型 | 苯丙素类(Phenylpropanoids)>香豆素类(Coumarins) |
化学式 | C10H8O4 |
分子量 | 192.17 |
CAS号 | 92-61-5 |
纯度 | 98%, HPLC |
溶剂/溶解度 | DMSO: ≥ 32 mg/mL (166.52 mM) |
溶液配制 | 2mg加入1.04mL DMSO,或者每1.92mg加入1mL DMSO,配制成10mM溶液。 |
产品描述 | Scopoletin is an inhibitor of acetylcholinesterase (AChE). | ||||
信号通路 | Apoptosis | ||||
靶点 | AChE | - | - | - | - |
IC50 | 4 μM | - | - | - | - |
体外研究 | Scopoletin (SCT) is identified as a putative inhibitor of acetylcholinesterase (AChE). Scopoletin enhances the K+-stimulated release of ACh from rat frontal cortex synaptosomes, showing a bell-shaped dose effect curve (Emax: 4 μM). Scopoletin inhibits PC3 proliferation by inducing apoptosis of PC3 cells. The IC50 of Scopoletin for inhibiting PC3, PAA (human lung cancer cell), and Hela cell proliferation is (157±25), (154±51), and (294±100) mg/L, respectively. Scopoletin induces a marked time- and concentration-dependent inhibition of PC3 cell proliferation. Scopoletin reduces the protein content and decreases the acid phosphatase activity (ACP) level in PC3 cells in a concentration-dependent manner. Cells treated by Scopoletin show typical morphologic changes of apoptosis by light microscope, fluorescence microscope, and transmission electronmicroscope. Apoptosis rate is 0.3 %, 2.1 %, 9.3 % and 35 % for Scopoletin 0, 100, 200, and 400 mg/L, respectively, and cells in G2 phase decrease markedly after being treated with Scopoletin. | ||||
体内研究 | Scopoletin (2 μg, i.c.v.) increases T-maze alternation and ameliorated novel object recognition of mice with scopolamine-induced cholinergic deficit. It also reduces age-associated deficits in object memory of 15-18-month-old mice (2 mg/kg sc). Mice injected with 2 μg Scopoletin show an increased alternation rate of 71.3±2.5%. | ||||
临床实验 | N/A |
1.Hornick A, et al. Neuroscience. 2011,197:280-92.
2.Liu XL, et al. Acta Pharmacol Sin. 2001,22(10):929-33.
包装清单:产品编号 | 产品名称 | 包装 |
SM1142-10mM | 东莨菪内酯(98%, HPLC) | 10mM×0.2ml |
SM1142-25mg | 东莨菪内酯(98%, HPLC) | 25mg |
SM1142-100mg | 东莨菪内酯(98%, HPLC) | 100mg |
- | 说明书 | 1份 |
-20℃保存,至少一年有效。固体粉末4℃保存,至少一个月有效。如果溶于非DMSO溶剂,建议分装后-80℃保存,预计6个月内有效。
注意事项:本产品可能对人体有一定的毒害作用,请注意适当防护,以避免直接接触人体或吸入体内。
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