化学信息：
| 化学名 | 8-phenyl-2-piperazin-1-ylchromen-4-one |
|
| 简称 | LY303511 | |
| 别名 |
LY-303511, LY303511 |
|
| 中文名 | N/A | |
| 化学式 | C19H18N2O2 | |
| 分子量 | 306.36 | |
| CAS号 | 154447-38-8 | |
| 纯度 | 98% | |
| 溶剂/溶解度 |
Water<1mg/ml; DMSO30.6mg/ml; Ethanol<1mg/ml |
|
| 溶液配制 | 5mg加入1.63ml DMSO，或者每3.06mg加入1ml DMSO，配制成10mM溶液。SF2816-10mM用DMSO配制。 |
生物信息：
| 产品描述 | LY303511, an inactive analogue of LY294002, is a mTOR inhibitor that did not inhibit PI3-K. | ||||
| 信号通路 | PI3K/Akt/mTOR | ||||
| 靶点 | － | － | － | － | － |
| IC50 | － | － | － | － | － |
| 体外研究 | 100μM LY303511 significantly reduced the fraction of cells in S phase. The proportion of cells in G2/M remained unchanged, indicating that cells were arrested in both G1 and G2/M. In contrast, rapamycin increased the G1 population by reducing the proportion of cells in both S and G2/M. The effects of 10μM LY303511 and rapamycin on the reduction in S phase cells were additive to that of 10μM LY303511 alone (P=0.056). In MIN6 insulinoma cells, wortmannin (100nM) had no effect on whole-cell outward K+ currents, but LY294002 and LY303511 reversibly blocked currents in a dose-dependent manner (IC50=9.0+/-0.7mM and 64.6+/-9.1mM, respectively). Western blotting confirmed the specific inhibitory effects of LY294002 and wortmannin on insulin-stimulated PI3K activity. Both LY294002 and LY303511 increased the activity of protein kinase A (PKA). Moreover, PKA blockade by the small molecule inhibitor H89 decreased the LY294002/LY303511-mediated increase in GJIC. | ||||
| 体内研究 | PND4 ovaries were cultured for 8 days in control medium or medium containing VCD (30μM) in the presence or absence of LY303511 (20μM). Incubation with LY303511 alone caused a reduction (P<0.05) in primordial and small primary follicle numbers. On the other hand, whereas VCD alone depleted (P<0.05) primordial and small primary follicle numbers, this depletion was not prevented by co-incubation with LY303511. | ||||
| 临床实验 | N/A | ||||
| 特征 | N/A | ||||
相关实验数据(此数据来自于公开文献，碧云天并不保证其有效性)：
| 酶活性检测实验 | |
| 方法 | N/A |
| 细胞实验 | |
| 细胞系 | N/A |
| 浓度 | N/A |
| 处理时间 | N/A |
| 方法 | N/A |
| 动物实验 | |
| 动物模型 | N/A |
| 配制 | N/A |
| 剂量 | N/A |
| 给药方式 | N/A |
| 产品编号 | 产品名称 | 包装 |
| SF2816-10mM | LY303511 (mTOR抑制剂) | 10mM×0.2ml |
| SF2816-5mg | LY303511 (mTOR抑制剂) | 5mg |
| SF2816-25mg | LY303511 (mTOR抑制剂) | 25mg |
| — | 说明书 | 1份 |
保存条件：
-20℃保存，至少一年有效。5mg和25mg包装也可室温保存，至少6个月有效。如果溶于非DMSO溶剂，建议分装后-80℃保存，预计6个月内有效。
