化学信息：
| 化学名 | 1-[2-(4-methoxyphenyl)-2-[3-(4-methoxyphenyl)propoxy]ethyl]imidazole;hydrochloride |
|
| 简称 | SKF96365 | |
| 别名 |
SK and F 96365, SK and F-96365, SKF-96365, SKF 96365, SKF 96365 HYDROCHLORIDE |
|
| 中文名 | N/A | |
| 化学式 | C22H26N2O3.HCl | |
| 分子量 | 402.91 | |
| CAS号 | 130495-35-1 | |
| 纯度 | 98% | |
| 溶剂/溶解度 |
Water80mg/ml; DMSO80mg/ml; Ethanol80mg/ml |
|
| 溶液配制 | 5mg加入1.24ml DMSO，或者每4.03mg加入1ml DMSO，配制成10mM溶液。SF1159-10mM用DMSO配制。 |
生物信息：
| 产品描述 | SKF96365, originally identified as a blocker of receptor-mediated calcium entry, is widely used diagnostically, as a blocker of transient receptor potential canonical type (TRPC) channels. | ||||
| 信号通路 | Others | ||||
| 靶点 | TRPC | － | － | － | － |
| IC50 | － | － | － | － | － |
| 体外研究 | SKF-96365 exhibits protective activity against MPP+ injury in PC12 cells and significantly inhibits apoptotic cell death in PC12 cells after MPP+ administration. SKF-96365 does not exert effects on necrotic cell death induced by MPP+ insult in PC12 cells. Because of its non-selective activity, SKF-96365 has been demonstrated to have effects on multiple other Ca2+ channels: it not only blocks high-voltage-activated (HVA) Ca2+ channels at typically utilized test concentrations, but also potently inhibits low-voltage -activated (LVA) T-type Ca2+ channels in HEK293 cells. The exact effect of SKF-96365 on intracellular calcium homeostasis might dependent on cell types and experimental models used. | ||||
| 体内研究 | SKF-96365 treatment inhibited the calcium/calmodulin-dependent protein kinase IIγ (CaMKIIγ)/AKT signaling cascade in vitro and in vivo. | ||||
| 临床实验 | N/A | ||||
| 特征 | N/A | ||||
相关实验数据(此数据来自于公开文献，碧云天并不保证其有效性)：
| 酶活性检测实验 | |
| 方法 | N/A |
| 细胞实验 | |
| 细胞系 | PC12 cells |
| 浓度 | 1μM, 10μM, 50μM |
| 处理时间 | 30 min |
| 方法 | To investigate whether SKF-96365 could protect PC12 cells from injury induced by MPP+ insult, cultured PC12 cells are pretreated with SKF-96365 in different concentrations (1μM, 10μM or 50μM) 30 min before MPP+ addition. The cells viability is measured 24h after MPP+ insult by using the cell proliferation reagent WST-1. |
| 动物实验 | |
| 动物模型 | C57BL/6N mice |
| 配制 | 0.9% sterile saline |
| 剂量 | 0.02μMol-0.2μMol/2μl/mouse |
| 给药方式 | intrastriatally |
| 产品编号 | 产品名称 | 包装 |
| SF1159-10mM | SKF96365 (TRPC抑制剂) | 10mM×0.2ml |
| SF1159-5mg | SKF96365 (TRPC抑制剂) | 5mg |
| SF1159-25mg | SKF96365 (TRPC抑制剂) | 25mg |
| — | 说明书 | 1份 |
保存条件：
-20℃保存，至少一年有效。5mg和25mg包装也可室温保存，至少6个月有效。如果溶于非DMSO溶剂，建议分装后-80℃保存，预计6个月内有效。
