化学信息：
| 化学名 | 3-(benzylsulfamoyl)-4-bromo-N-(4-bromophenyl)benzamide |
|
| 简称 | RS1 | |
| 别名 |
RS-1, RS 1 |
|
| 中文名 | N/A | |
| 化学式 | C20H16Br2N2O3S | |
| 分子量 | 524.23 | |
| CAS号 | 312756-74-4 | |
| 纯度 | 98% | |
| 溶剂/溶解度 |
Water<1mg/ml; DMSO100mg/ml; Ethanol<1mg/ml |
|
| 溶液配制 | 5mg加入0.95ml DMSO，或者每5.24mg加入1ml DMSO，配制成10mM溶液。SF1069-10mM用DMSO配制。 |
生物信息：
| 产品描述 | RS-1 is a RAD51-stimulatory compound, which increases the DNA binding activity of RAD51. It is an HDR(homology-directed repair) enhancer. | ||||
| 信号通路 | Others | ||||
| 靶点 | RAD51 | － | － | － | － |
| IC50 | － | － | － | － | － |
| 体外研究 | RS-1 stimulates the binding of hRAD51 to ssDNA. Low-micromolar concentrations of this small molecule enhance DNA binding and result in longer protein–DNA complex lengths. In addition, RS-1 stabilizes the active form of hRAD51 filaments and this is reflected in an enhanced strand assimilation activity. | ||||
| 体内研究 | N/A | ||||
| 临床实验 | N/A | ||||
| 特征 | N/A | ||||
相关实验数据(此数据来自于公开文献，碧云天并不保证其有效性)：
| 酶活性检测实验 | |
| 方法 | N/A |
| 细胞实验 | |
| 细胞系 | Early-passage neonatal human dermal fibroblasts |
| 浓度 | 1-7.5μM |
| 处理时间 | 24h |
| 方法 | Early-passage neonatal human dermal fibroblasts were maintained in Medium 106 supplemented with low serum growth supplement. Cell-survival experiments were performed using a sulforhodamine B assay. Cells are incubated for 24h in cisplatin with or without RS-1 and are subsequently incubated for an additional 6 days in normal media lacking both agents. Sulforhodamine B-stained cells are quantified with a Synergy plate reader. |
| 动物实验 | |
| 动物模型 | N/A |
| 配制 | N/A |
| 剂量 | N/A |
| 给药方式 | N/A |
| 产品编号 | 产品名称 | 包装 |
| SF1069-10mM | RS1 (RAD51激动剂) | 10mM×0.2ml |
| SF1069-5mg | RS1 (RAD51激动剂) | 5mg |
| SF1069-25mg | RS1 (RAD51激动剂) | 25mg |
| — | 说明书 | 1份 |
保存条件：
-20℃保存，至少一年有效。5mg和25mg包装也可室温保存，至少6个月有效。如果溶于非DMSO溶剂，建议分装后-80℃保存，预计6个月内有效。
