碧云天-S1052-Fura-2 AM (钙离子荧光探针, 2mM)

Fura-2 AM (钙离子荧光探针, 2mM)

赠

取消收藏

：

产品编号： S1052

数量：

¥ 329.00

产品包装:

50μl

					产品简介
				
					使用说明
				
					相关文件下载

					相关产品
				
					相关论文
				
					产品问答

Fura-2 AM，也称Fura-2 pentakis(acetoxymethyl) ester，是最常用的检测细胞内钙离子浓度的荧光探针之一。分子式为
C₄₄H₄₇N₃O₂₄，分子量为1001.9。
本Fura-2 AM(钙离子荧光探针)是配制于无水DMSO(anhydrous DMSO)中的储存液，浓度为2mM。
Fura-2 AM是一种可以穿透细胞膜的荧光染料。Fura-2 AM的荧光比较弱，最大激发波长为369nm，最大发射波长为478nm，并且其荧光不会随钙离子浓度改变而改变。Fura-2 AM进入细胞后可以被细胞内的酯酶剪切形成Fura-2，从而被滞留在细胞内。Fura-2可以和钙离子结合，结合钙离子后在330-350nm激发光下可以产生较强的荧光，而在380nm激发光下则会导致荧光减弱。这样就可以使用340nm和380nm这两个荧光的比值来检测细胞内的钙离子浓度，可以消除不同细胞样品间荧光探针装载效率的差异，荧光探针的渗漏，细胞厚度差异等一些误差因素。Fura-2和钙离子结合后，最大激发波长为335nm(最大激发波长随离子浓度的的不同而有所不同)，最大发射波长为505nm。实际检测时推荐使用的激发波长为340nm，发射波长为510nm。如果做双波长检测，则推荐使用的激发波长为340nm和380nm。Fura-2的激发光谱参考下图，不同曲线表示不同钙离子浓度时的激发光谱。

Fura-2相对而言有较强的抗荧光淬灭能力，在荧光显微镜或其它荧光检测设备上可以连续检测1小时而不明显影响其荧光效果。

用于细胞内钙离子检测时，Fura-2 AM的常用浓度为0.5-5μM。通常用含有0.5-5μM的Fura-2 AM的适当溶液和细胞一起在4-37℃孵育15-60分钟，即可完成荧光探针的装载。
包装清单：

产品编号	产品名称	包装
S1052	Fura-2 AM(钙离子荧光探针, 2mM)	50μl
—	说明书	1份

保存条件：
-20℃避光保存，6个月有效。
注意事项：
本Fura-2 AM在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内，可以20-25℃水浴温育片刻至全部融解后使用。
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

说明书下载

相关产品请点击如下按钮：

钙离子等检测

小分子荧光探针

使用本产品的文献：

1. Zunyong Feng, Jing Zhou, Yinhua Liu, Ruixue Xia, Qiang Li, Liang Yan, Qun Chen, Xiaobing Chen, Yuxin Jiang, Gao Chao, Ming Wang, Guoren Zhou, Yijie Zhang, Yongsheng Wang, Hongping Xia

Epithelium- and endothelium-derived exosomes regulate the alveolar macrophages by targeting RGS1 mediated calcium signaling-dependent immune response

Cell Death Differ. doi: 10.1038/s41418-021-00750-x. (IF 10.717)

2. Xue-Chun Ni, Hong-Fei Wang, Yuan-Yuan Cai, Dai Yang, Raphael N Alolga, Baolin Liu, Jia Li, Feng-Qing Huang

Ginsenoside Rb1 inhibits astrocyte activation and promotes transfer of astrocytic mitochondria to neurons against ischemic stroke

Redox Biol. doi: 10.1016/j.redox.2022.102363. (IF 9.986)

3. Guo RW, Wang H, Gao P, Li MQ, Zeng CY, Yu Y, Chen JF, Song MB, Shi YK, Huang L.

An essential role for stromal interaction molecule 1 in neointima formation following arterial injury.

Cardiovasc Res 2009 Mar 1;81(4):660-8. (IF 8.168)

4. Zhang Y, Yin H, Zhao X, Wang W, Du Y, He A, Sun K.

The promoting effects of alginate oligosaccharides on root development in Oryza sativa L. mediated by auxin signaling.

CARBOHYD POLYM 2014 Nov 26;113:446-54. (IF 7.182)

5. Mingquan Huang, Xingjie Li, Shengjun Jia, Shuang Liu, Li Fu, Xue Jiang, Meng Yang

Bisphenol AF induces apoptosis via estrogen receptor beta (ERβ) and ROS-ASK1-JNK MAPK pathway in human granulosa cell line KGN

Environ Pollut. doi: 10.1016/j.envpol.2020.116051. (IF 6.792)

6. Ping Xiang, Kun Wang, Jue Bi, Mengying Li, Rui-Wen He, Daolei Cui, Lena Q Ma

Organic extract of indoor dust induces estrogen-like effects in human breast cancer cells

Sci Total Environ. doi: 10.1016/j.scitotenv.2020.138505. (IF 6.551)

7. Li C, Yang Z, Li Z, Ma Y, Zhang L, Zheng C, Qiu W, Wu X, Wang X, Li H, Tang J, Qian M, Li D, Wang P, Luo J, Liu M.

Maslinic acid suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss by regulating RANKL-mediated NF-κB and MAPK signaling pathways.

J Bone Miner Res 2011 Mar;26(3):644-56. (IF 5.854)

8. Fenghua Wei, Tenghui Su, Dali Wang, Huizhen Li, Jing You

Transcriptomic analysis reveals common pathways and biomarkers associated with oxidative damage caused by mitochondrial toxicants in Chironomus dilutus

Chemosphere. doi: 10.1016/j.chemosphere.2020.126746. (IF 5.778)

9. Sun ZL, Niu RY, Su K, Wang B, Wang JM, Zhang JH, Wang JD.

Effects of sodium fluoride on hyperactivation and Ca 2+ signaling pathway in sperm from mice: an in vivo study.

ARCHIVES OF TOXICOLOGY 2010;84(5):353-61. (IF 5.741)

10. Yan Wang, Naijian Li, Yingfeng Wang, Guobing Zheng, Jing An, Chang Liu, Yajie Wang, Qicai Liu

NF-κB/p65 Competes With Peroxisome Proliferator-Activated Receptor Gamma for Transient Receptor Potential Channel 6 in Hypoxia-Induced Human Pulmonary Arterial Smooth Muscle Cells

Front Cell Dev Biol. doi: 10.3389/fcell.2021.656625. (IF 5.201)

11. Zhou R, Ding XL, Liu LM.

Ryanodine receptor 2 contributes to hemorrhagic shock-induced bi-phasic vascular reactivity in rats.

Acta Pharmacol Sin 2014 Nov;35(11):1375-84. (IF 5.064)

12. Ji H, Wang L, Bi H, Sun L, Cai B, Wang Y, Zhao J, Du Z.

Mechanisms of lumbrokinase in protection of cerebral ischemia.

Eur J Pharmacol 2008 Aug 20;590(1-3):281-9. (IF 3.263)

13. Bi J, Wang XB, Chen L, Hao S, An LJ, Jiang B, Guo L.

Catalpol protects mesencephalic neurons against MPTP induced neurotoxicity via attenuation of mitochondrial dysfunction and MAO-B activity.

Toxicol In Vitro 2008 Dec;22(8):1883-9. (IF 2.959)

14. Yang LX, Guo RW, Liu B, Wang XM, Qi F, Guo CM, Shi YK, Wang H.

Role of TRPC1 and NF-kappaB in mediating angiotensin II-induced Ca2+ entry and endothelial hyperpermeability.

Peptides 2009 Jul;30(7):1368-73. (IF 2.843)

15. Shen W, Song D, Wu J, Zhang W.

Protective effect of a polysaccharide isolated from a cultivated Cordyceps mycelia on hydrogenperoxide-induced oxidative damage in PC12 cells.

Phytother Res 2011 May;25(5):675-80. (IF 4.087)

16. Sun C, Wang L, Yan J, Liu S.

Calcium ameliorates obesity induced by high-fat diet and its potential correlation with p38 MAPKpathway.

Mol Biol Rep 2012 Feb;39(2):1755-63. (IF 1.402)

17. Zheng B, Wu L, Ma L, Liu S, Li L, Xie W, Li X.

Telekin induces apoptosis associated with the mitochondria-mediated pathway in human hepatocellular carcinoma cells.

Biol Pharm Bull 2013;36(7):1118-25. (IF 1.863)

18. Zhou J, Yang C, Wang J, Sun P, Fan P, Tian K, Liu S, Xia C.

Toxic effects of environment-friendly antifoulant nonivamide on Phaeodactylum tricornutum.

Environ Toxicol Chem 2013 Apr;32(4):802-9. (IF 3.152)

19. Liu H, Han F, Shi Y.

Effect of Calreticulin on Ca(2+)/CaM KinaseIIα and Endoplasmic Reticulum Stress in Hippocampal in a Rat Model of Post-traumatic Stress Disorder.

Neurochem Res 2013 Jul;38(7):1407-14. (IF 3.038)

20. Wang H, Yin G, Yu CH, Wang Y, Sun Z.

Inhibitory effect of sanguinarine on PKC-CPI-17 pathway mediating by muscarinic receptors in dispersed intestinal smooth muscle cells.

Res Vet Sci 2013 Dec;95(3):1125-33. (IF 1.892)

21. Liu J, Zhang Y, Li Q, Zhuang Q, Zhu X, Pan L, Cheng M.

An improved method for guinea pig airway smooth muscle cell culture and the effect of SPFF on intracellular calcium.

Mol Med Rep 2014 Sep;10(3):1309-14. (IF 2.1)

22. Zhong L, Lv L, Yang J, Liao X, Yu J, Wang R, Zhou P.

Inhibitory effect of hydrogen sulfide on platelet aggregation and the underlying mechanisms.

J CARDIOVASC PHARM 2014 Nov;64(5):481-7. (IF 2.598)

23. Ren H, Teng Y, Tan B, Zhang X, Jiang W, Liu M, Jiang W, Du B, Qian M.

Toll-like receptor-triggered calcium mobilization protects mice against bacterial infection through extracellular ATP release.

Infect Immun 2014 Dec;82(12):5076-85. (IF 3.201)

24. Liu Z, Cai H, Zhu H, Toque H, Zhao N, Qiu C, Guan G, Dang Y, Wang J.

Protein kinase RNA-like endoplasmic reticulum kinase (PERK)/calcineurin signaling is a novel pathway regulating intracellular calcium accumulation which might be involved in ventricular arrhythmias in diabetic cardiomyopathy.

Cell Signal 2014 Dec;26(12):2591-600. (IF 3.968)

25. Liu Y, Shen S, Li Z, Jiang Y, Si J, Chang Q, Liu X, Pan R.

Cajaninstilbene acid protects corticosterone-induced injury in PC12 cells by inhibiting oxidative and endoplasmic reticulum stress-mediated apoptosis.

Neurochem Int 2014 Dec;78:43-52. (IF 3.881)

26. Wen L, Han F, Shi Y.

Changes in the glucocorticoid receptor and Ca2⁺/calreticulin-dependent signalling pathway in the medial prefrontal cortex of rats with post-traumatic stress disorder.

J Mol Neurosci 2015 May;56(1):24-34. (IF 2.678)

27. Xu X, Chen D, Ye B, Zhong F, Chen G.

Curcumin induces the apoptosis of non-small cell lung cancer cells through a calcium signaling pathway.

Int J Mol Med 2015 Jun;35(6):1610-6. (IF 3.098)

28. Li H, Feng L, Jiang W, Liu Y, Jiang J, Zhang Y, Wu P, Zhou X.

Ca(2+) and caspases are involved in hydroxyl radical-induced apoptosis in erythrocytes of Jian carp (Cyprinus carpio var. Jian).

Fish Physiol Biochem 2015 Oct;41(5):1305-19. (IF 2.242)

29. Song X, Zhao C, Dai C, Ren Y, An N, Wen H, Pan LI, Cheng M, Zhang Y.

Suppression of the increasing level of acetylcholine-stimulated intracellular Ca2+ in guinea pig airway smooth muscle cells by mabuterol.

Biomed Rep. 2015 Nov;3(6):778-86.

30. Wei NN, Lv HN, Wu Y, Yang SL, Sun XY, Lai R, Jiang Y, Wang K.

Selective Activation of Nociceptor TRPV1 Channel and Reversal of Inflammatory Pain in Mice by a Novel Coumarin Derivative Muralatin L from Murraya alata.

J Biol Chem 2016 Jan 8;291(2):640-51. (IF 4.238)

31. Cao Z, Liu D, Zhang Q, Sun X, Li Y.

Aluminum Chloride Induces Osteoblasts Apoptosis via Disrupting Calcium Homeostasis and Activating Ca2+/CaMKII Signal Pathway.

Biol Trace Elem Res 2016 Feb;169(2):547-53. (IF 2.639)

32. Qiu Y, Yu Q, Shi K, Zhang M, Zhou X, Yang Y, Liu Y, Tang J, Wang X, He Q.

Cell-penetrating peptides induce apoptosis and necrosis through specific mechanism and causeimpairment of Na+-K+-ATPase and mitochondria.

Amino Acids 2017 Jan;49(1):75-88. (IF 3.063)

33. Wang B, Zhao XH.

Apigenin induces both intrinsic and extrinsic pathways of apoptosis in human colon carcinoma HCT-116cells.

Oncol Rep 2017 Feb;37(2):1132-1140. (IF 3.417)

34. Liu Z, Zhang Y, Tang Z, Xu J, Ma M, Pan S, Qiu C, Guan G, Wang J.

Matrine attenuates cardiac fibrosis by affecting ATF6 signaling pathway in diabeticcardiomyopathy.

Eur J Pharmacol 2017 Jun 5;804:21-30. (IF 3.263)

35. Liu Y, Zhao N, Li C, Chang Q, Liu X, Liao Y, Pan R.

Longistyline C acts antidepressant in vivo and neuroprotection in vitro against glutamate-induced cytotoxicity by regulating NMDAR/NR2B-ERK pathway in PC12 cells.

PLoS One 2017 Sep 5;12(9):e0183702. (IF 2.74)

36. Jiang Y, Jiao Y, Liu Y, Zhang M, Wang Z, Li Y, Li T, Zhao X, Wang D.

Sinomenine Hydrochloride Inhibits the Metastasis of Human Glioblastoma Cells by Suppressing the Expression of Matrix Metalloproteinase-2/-9 and Reversing the Endogenous and Exogenous Epithelial-Mesenchymal Transition.

Int J Mol Sci 2018 Mar 14;19(3). pii: E844. (IF 4.556)

37. Wang J, Tang Z, Zhang Y, Qiu C, Zhu L, Zhao N, Liu Z

Matrine alleviates AGEs- induced cardiac dysfunctions by attenuating calcium overload via reducing ryanodine receptor 2 activity.

Eur J Pharmacol. 842:118-124. (IF 3.263)

38. Zhou H, Zhang YQ, Lai T, Liu XJ, Guo FY, Guo T, Ding W.

Acaricidal Mechanism of Scopoletin Against Tetranychus cinnabarinus.

Front Physiol. 10:164. (IF 3.367)

39. He D, Wang N, Sai X, Li X, Xu Y

Camellia euphlebia protects against corticosterone-induced apoptosis in differentiated PC12 cells by regulating the mitochondrial apoptotic pathway and PKA/CREB/BDNF signaling pathway.

Food Chem Toxicol. 126:211-222. (IF 4.679)

40. Kong W, Wang X, Yang X, Huang W, Han S, Yin J, Liu W, He X, Peng B

Activation of TRPV1 Contributes to Recurrent Febrile Seizures via Inhibiting the Microglial M2 Phenotype in the Immature Brain.

Front Cell Neurosci. 13:442. (IF 3.921)

41. Cui D, Yin Y, Wang J, Wang Z, Ding H, Ma R, Jiao Z

Research on the Physio-Biochemical Mechanism of Non-Thermal Plasma-Regulated Seed Germination and Early Seedling Development in Arabidopsis.

Front Plant Sci. 10:1322. (IF 4.402)

42. Ya Ma, Haohao Liu, Xingde Du, Pavankumar Petlulu, Xinghai Chen, Rui Wang, Shiyu Zhang, Zhihui Tian, Linjia Shi, Hongxiang Guo, Huizhen Zhang

IRE1 and CaMKKβ pathways to reveal the mechanism involved in microcystin-LR-induced autophagy in mouse ovarian cells

Food Chem Toxicol. doi: 10.1016/j.fct.2020.111911. (IF 4.679)

43. Ting Yu, Enze Zheng, Yanping Li, Yuqi Li, Jun Xia, Qiuying Ding, Zhengping Hou, Xiong Z Ruan, Lei Zhao, Yaxi Chen

Src-mediated Tyr353 phosphorylation of IP3R1 promotes its stability and causes apoptosis in palmitic acid-treated hepatocytes

Exp Cell Res. doi: 10.1016/j.yexcr.2020.112438. (IF 3.383)

44. Xiangrong Cui, Hongping Liang, Chonghua Hao, Xuan Jing

Liraglutide preconditioning attenuates myocardial ischemia/ reperfusion injury via homer1 activation

Aging (Albany NY). doi: 10.18632/aging.202429.

45. Lu Fu, Hongyuan Zhang, Jeremiah Ong'achwa Machuki, Tingting Zhang, Lin Han, Lili Sang, Lijuan Wu, Zhiwei Zhao, Matthew James Turley, Xide Hu, Hongjian Hou, Dongye Li, Sian E Harding, Hong Sun

GPER mediates estrogen cardioprotection against epinephrine-induced stress

J Endocrinol. doi: 10.1530/JOE-20-0451. (IF 4.041)

46. J Wang, D Cui, L Wang, M Du, Y Yin, R Ma, H Sun, Z Jiao

Atmospheric pressure plasma treatment induces abscisic acid production, reduces stomatal aperture and improves seedling growth in Arabidopsis thaliana

Plant Biol (Stuttg). doi: 10.1111/plb.13245.

47. Juan Jin, Mei Li, Jing Li, Bin Li, Lei Duan, Fang Yang, Ning Gu

Xenon Nanobubbles for the Image-Guided Preemptive Treatment of Acute Ischemic Stroke via Neuroprotection and Microcirculatory Restoration

ACS Appl Mater Interfaces. doi: 10.1021/acsami.1c06014.

48. Ting-Ting Huang, Pan-Pan Gu, Ting Zheng, Ling-Shan Gou, Yao-Wu Liu

Piperine, as a TAS2R14 agonist, stimulates the secretion of glucagon-like peptide-1 in the human enteroendocrine cell line Caco-2

Food Funct. doi: 10.1039/d1fo02932k. (IF 4.171)

49. Weifan Hu, Yongbo Yu, Yang Sun, Feng Yuan, Fengchao Zhao