碧云天-S1052-Fura-2 AM (钙离子荧光探针, 2mM)

Fura-2 AM (钙离子荧光探针, 2mM)

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产品编号： S1052

数量：

¥ 329.00 元

产品包装:

50μl

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Fura-2 AM，也称Fura-2 pentakis(acetoxymethyl) ester，是最常用的检测细胞内钙离子浓度的荧光探针之一。分子式为
C₄₄H₄₇N₃O₂₄，分子量为1001.9。
本Fura-2 AM(钙离子荧光探针)是配制于无水DMSO(anhydrous DMSO)中的储存液，浓度为2mM。
Fura-2 AM是一种可以穿透细胞膜的荧光染料。Fura-2 AM的荧光比较弱，最大激发波长为369nm，最大发射波长为478nm，并且其荧光不会随钙离子浓度改变而改变。Fura-2 AM进入细胞后可以被细胞内的酯酶剪切形成Fura-2，从而被滞留在细胞内。Fura-2可以和钙离子结合，结合钙离子后在330-350nm激发光下可以产生较强的荧光，而在380nm激发光下则会导致荧光减弱。这样就可以使用340nm和380nm这两个荧光的比值来检测细胞内的钙离子浓度，可以消除不同细胞样品间荧光探针装载效率的差异，荧光探针的渗漏，细胞厚度差异等一些误差因素。Fura-2和钙离子结合后，最大激发波长为335nm(最大激发波长随离子浓度的的不同而有所不同)，最大发射波长为505nm。实际检测时推荐使用的激发波长为340nm，发射波长为510nm。如果做双波长检测，则推荐使用的激发波长为340nm和380nm。Fura-2的激发光谱参考下图，不同曲线表示不同钙离子浓度时的激发光谱。

Fura-2相对而言有较强的抗荧光淬灭能力，在荧光显微镜或其它荧光检测设备上可以连续检测1小时而不明显影响其荧光效果。

用于细胞内钙离子检测时，Fura-2 AM的常用浓度为0.5-5μM。通常用含有0.5-5μM的Fura-2 AM的适当溶液和细胞一起在4-37℃孵育15-60分钟，即可完成荧光探针的装载。
包装清单：

产品编号	产品名称	包装
S1052	Fura-2 AM(钙离子荧光探针, 2mM)	50μl
—	说明书	1份

保存条件：
-20℃避光保存，6个月有效。
注意事项：
本Fura-2 AM在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内，可以20-25℃水浴温育片刻至全部融解后使用。
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

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钙离子等检测

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