Dihydroethidium (超氧化物阴离子荧光探针)
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产品编号 ： S0063
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¥ 452.00
产品包装:
5mg
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Dihydroethidium简称DHE，是最常用的检测细胞内超氧化物阴离子水平的荧光探针。分子式为C21H21N3，分子量为
315.41。
Dihydroethidium是一种最常用的超氧化物阴离子荧光检测探针。可以直接用于活细胞的标记。Dihydroethidium被活细胞摄入后，可以在细胞内的超氧化物阴离子作用下脱氢，产生ethidium。Ethidium (例如溴化乙锭)可以和RNA或DNA结合产生红色荧光。当细胞内的超氧化物阴离子水平较高时，产生的ethidium较多，红色荧光就较强，反之则较弱。这样就可以用dihydroethidium进行超氧化物阴离子水平的检测。
Dihydroethidium本身为蓝色荧光，最大激发波长为370nm，最大发射波长为420nm，脱氢后和RNA或DNA结合产生红色荧光，最大激发波长为300nm，最大发射波长为610nm，实际观察时也可以使用535nm作为激发波长。
Dihydroethidium可以溶解于DMSO，配制成适当的母液。
用于细胞内超氧化物阴离子检测时，Dihydroethidium的常用浓度为1-10μM。通常用含有0.5-5μM的Dihydroethidium的适当溶液和细胞一起在37℃孵育30分钟左右进行荧光探针装载，随后可以适当洗涤，接着可以直接用流式细胞仪或其它适当荧光检测仪器进行检测。
包装清单：

产品编号 产品名称 包装
S0063 Dihydroethidium(超氧化物阴离子荧光探针) 5mg
— 说明书 1份

保存条件：
-20℃避光保存，6个月有效。易在空气中氧化，尽量避免暴露于空气中。
注意事项：
Dihydroethidium易在空气中氧化，请尽量避免暴露于空气中。
Dihydroethidium脱氢后产生毒性较高的ethidium，请注意防护。
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
      本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。 

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S0064S
100-1000次
超氧阴离子活性氧检测试剂盒(DHE)
S0033S
>100次
活性氧检测试剂盒
S0061S
20-200次
线粒体超氧化物检测试剂盒(MitoSO™ Red)
S0035S
20-200次
活性氧检测试剂盒(CM-H2DCFDA)
S0033M
>500次
活性氧检测试剂盒
S0067-100μg
100μg
SOSG(单线态氧绿色荧光探针)
C2006
>100次
线粒体膜电位检测试剂盒(JC-1)
S0060
100次
超氧化物检测试剂盒
S0034S
>100次
活性氧检测试剂盒(含稀释液)
S0131S
100次
脂质氧化(MDA)检测试剂盒
S0043S
100-1000次
脂质过氧化检测试剂盒(BODIPY 581/591 C11)
S0019S
>100次
DAF-FM DA (NO荧光探针)