Dihydroethidium (超氧化物阴离子荧光探针)
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产品编号 ： S0063
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¥ 452.00 元
产品包装:
5mg
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Dihydroethidium简称DHE，是最常用的检测细胞内超氧化物阴离子水平的荧光探针。分子式为C21H21N3，分子量为
315.41。
Dihydroethidium是一种最常用的超氧化物阴离子荧光检测探针。可以直接用于活细胞的标记。Dihydroethidium被活细胞摄入后，可以在细胞内的超氧化物阴离子作用下脱氢，产生ethidium。Ethidium (例如溴化乙锭)可以和RNA或DNA结合产生红色荧光。当细胞内的超氧化物阴离子水平较高时，产生的ethidium较多，红色荧光就较强，反之则较弱。这样就可以用dihydroethidium进行超氧化物阴离子水平的检测。
Dihydroethidium本身为蓝色荧光，最大激发波长为370nm，最大发射波长为420nm，脱氢后和RNA或DNA结合产生红色荧光，最大激发波长为300nm，最大发射波长为610nm，实际观察时也可以使用535nm作为激发波长。
Dihydroethidium可以溶解于DMSO，配制成适当的母液。
用于细胞内超氧化物阴离子检测时，Dihydroethidium的常用浓度为1-10μM。通常用含有0.5-5μM的Dihydroethidium的适当溶液和细胞一起在37℃孵育30分钟左右进行荧光探针装载，随后可以适当洗涤，接着可以直接用流式细胞仪或其它适当荧光检测仪器进行检测。
包装清单：

产品编号 产品名称 包装
S0063 Dihydroethidium(超氧化物阴离子荧光探针) 5mg
— 说明书 1份

保存条件：
-20℃避光保存，6个月有效。易在空气中氧化，尽量避免暴露于空气中。
注意事项：
Dihydroethidium易在空气中氧化，请尽量避免暴露于空气中。
Dihydroethidium脱氢后产生毒性较高的ethidium，请注意防护。
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
      本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。 

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429. Kai Xu, Xiang Liu, Bin Wen, Yazhou Liu, Wei Zhang, Xiaolin Hu, Ling Chen, Weijian Hang, Juan Chen
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431. Meng-Ru Gao, Peng Zhang, Jing Han, Chun-Lei Tang, Yi-Fei Zhu, Hao Kan, Hong-Juan Li, Xi-Ping Han, Xin Ma
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433. Ran Sun, Xuedong Wang, Yu Nie, Ao Hu, Hanrui Liu, Kun Zhang, Lu Zhang, Qihong Wu, Kuan Li, Chuchu Liu, Huan Zhang, Bingwen Zheng, Hong Li, Huayan Xu, Rong Xu, Hang Fu, Li Dai, Rongrong Jin, Yingkun Guo
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434. Chun Quan Zhu, QianQian Wei, Wen Jun Hu, Ya Li Kong, Xing Jia Xiang, Hui Zhang, Xiao Chuang Cao, Lian Feng Zhu, Jia Liu, Wen Hao Tian, Qian Yu Jin, Jun Hua Zhang
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437. Hong-Jie Yang, Bin Kong, Wei Shuai, Jing-Jing Zhang, He Huang
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438. Yun He, Hongyu Su, Na Li, Yingying Zhang, Pengjie Zhang, Yumeng Zhang, Yang Ye, Yueming Zhang, Jiaqi Tang, Zhice Xu
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441. Xiaoyang Cui, Enhua Shi, Jing Li, Yujia Li, Zhe Qiao, Ziying Wang, Min Liu, Wei Tang, Yu Sun, Yan Zhang, Yusheng Xie, Junhui Zhen, Xiaojie Wang, Fan Yi
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443. Baofu Zhang, Wenming Bao, Sina Zhang, Bo Chen, Xiang Zhou, Jungang Zhao, Zhehao Shi, Tan Zhang, Ziyan Chen, Luhui Wang, Xiangtao Zheng, Gang Chen, Yi Wang
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S0033S
>100次
活性氧检测试剂盒
S0061S
20-200次
线粒体超氧化物检测试剂盒(MitoSOX Red)
S0033M
>500次
活性氧检测试剂盒
S0067-100μg
100μg
SOSG(单线态氧绿色荧光探针)
S0064S
100-1000次
超氧阴离子活性氧检测试剂盒(DHE)
S0060
100次
超氧化物检测试剂盒
C2006
>100次
线粒体膜电位检测试剂盒(JC-1)
S0035S
20-200次
活性氧检测试剂盒(CM-H2DCFDA)
S0131S
100次
脂质氧化(MDA)检测试剂盒
C2003S
>100次
增强型线粒体膜电位检测试剂盒(JC-1)
S0053
共100次
GSH和GSSG检测试剂盒
S0038
150次
过氧化氢检测试剂盒