活性氧检测试剂盒
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产品编号 S0033M
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¥ 1542.00
产品包装:
>100次 >500次
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活性氧检测试剂盒(Reactive Oxygen Species Assay Kit,也称ROS Assay Kit)是一种利用荧光探针DCFH-DA进行活性氧检测的试剂盒。DCFH-DA本身没有荧光,可以自由穿过细胞膜,进入细胞内后,可以被细胞内的酯酶水解生成DCFH。而DCFH不能通透细胞膜,从而使探针很容易被装载到细胞内。细胞内的活性氧可以氧化无荧光的DCFH生成有荧光的DCF。检测DCF的荧光就可以知道细胞内活性氧的水平[1, 2]。
本试剂盒提供了活性氧阳性对照试剂Rosup,以便于活性氧的检测。Rosup是一种混合物(compound mixture),浓度为50mg/ml。使用本试剂盒检测细胞内活性氧的效果请参考图1。
图1. 碧云天活性氧检测试剂盒(S0033)检测L929 (小鼠成纤维细胞)细胞内活性氧的效果图。L929细胞装载DCFH-DA探针后用50μg/ml Rosup处理30分钟,随后PBS洗涤一次后用荧光显微镜(图A)检测。正常的L929细胞中探针几乎未被氧化成荧光化合物,细胞中的绿色荧光非常弱;使用活性氧阳性对照试剂Rosup处理使细胞内活性氧生成大幅增加,DCFH-DA与活性氧反应,细胞中的绿色荧光显著增强。实际结果会因实验条件、检测仪器等的不同而存在差异,图中效果仅供参考。
本试剂盒本底低,灵敏度高,线性范围宽,使用方便。
本试剂盒S0033S包装可以测定100-500个样品,S0033M包装可以测定500-2500个样品。
包装清单:

产品编号 产品名称 包装
S0033S-1 DCFH-DA(10mM) 0.1ml
S0033S-2 活性氧阳性对照(Rosup, 50mg/ml) 1ml
说明书 1份

产品编号 产品名称  包装 
S0033M-1 DCFH-DA(10mM) 0.5ml
S0033M-2 活性氧阳性对照(Rosup, 50mg/ml) 5ml
说明书 1份

保存条件:
-20℃保存,一年有效。
注意事项:
探针装载后,一定要洗净残余的未进入细胞内的探针,否则会导致背景较高。
探针装载完毕并洗净残余探针后,可以进行激发波长的扫描和发射波长的扫描,以确认探针的装载情况是否良好。DCF的激发光谱和发射光谱请参考下页图谱。
尽量缩短探针装载后到测定所用的时间(刺激时间除外),以减少各种可能的误差。
荧光酶标仪检测时须使用适合荧光检测的黑板或白板,推荐使用碧云天BeyoGold™全黑96孔细胞培养板(FCP966)或BeyoGold™黑色透明底96孔细胞培养板(平底带盖, 独立包装) (FCP965)。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 装载探针
对于刺激时间较短(通常为2小时以内)的细胞,先装载探针,后用活性氧阳性对照或自己感兴趣的药物刺激细胞。对于细胞刺激时间较长(通常为6小时以上)的细胞,先用活性氧阳性对照或自己感兴趣的药物刺激细胞,后装载探针。
原位装载探针:本方法仅适用于贴壁培养细胞。按照1:1000用细胞外液(C0216)或酌情使用PBS、HBSS等适当溶液稀释DCFH-DA,使终浓度为10微摩尔/升。去除细胞培养液,加入适当体积稀释好的DCFH-DA。加入的体积以能充分盖住细胞为宜,通常对于六孔板的一个孔加入稀释好的DCFH-DA不少于1毫升。37ºC细胞培养箱内孵育20分钟。用细胞外液(C0216)或酌情使用PBS、HBSS、无血清细胞培养液等适当溶液洗涤细胞三次,以充分去除未进入细胞内的DCFH-DA。通常活性氧阳性对照在刺激细胞20-30分钟后可以显著提高活性氧水平。
收集细胞后装载探针:按照1:1000用细胞外液(C0216)或酌情使用PBS、HBSS等适当溶液稀释DCFH-DA,使终浓度为10微摩尔/升。细胞收集后悬浮于稀释好的DCFH-DA中,细胞浓度为一百万至二千万/毫升,37ºC细胞培养箱内孵育20分钟。每隔3-5分钟颠倒混匀一下,使探针和细胞充分接触。用细胞外液(C0216)或酌情使用PBS、HBSS、无血清细胞培养液等适当溶液洗涤细胞三次,以充分去除未进入细胞内的DCFH-DA。直接用活性氧阳性对照或自己感兴趣的药物刺激细胞,或把细胞等分成若干份后刺激细胞。通常活性氧阳性对照在刺激细胞20-30分钟后可以显著提高活性氧水平。
说明:仅在阳性对照孔中加入Rosup作为阳性对照,其余孔不必加入Rosup。细胞外液推荐使用细胞外液(Extracellular Solution) (C0216),PBS推荐使用PBS (C0221A),HBSS推荐使用Hanks' Balanced Salt Solution (C0218)。
2. 检测
对于原位装载探针的样品可以用激光共聚焦显微镜直接观察,或收集细胞后用荧光分光光度计、荧光酶标仪或流式细胞仪检测。对于收集细胞后装载探针的样品可以用荧光分光光度计、荧光酶标仪或流式细胞仪检测,用激光共聚焦显微镜直接观察也可以。
3. 参数设置
使用488nm激发波长,525nm发射波长,实时或逐时间点检测刺激前后荧光的强弱。DCF的荧光光谱和FITC非常相似,可以用FITC的参数设置检测DCF。DCF的激发光谱和发射光谱参考下图。

4. 其它说明
阳性对照Rosup可以按照1:1000的比例酌情使用PBS、HBSS、细胞外液或无血清培养液稀释后使用。例如装载好探针的细胞,直接吸除液体或离心后吸除液体,加入适当体积的按照1:1000稀释的Rosup阳性对照刺激。通常刺激后20-30分钟内可以观察到非常显著的活性氧水平升高。对于不同的细胞,活性氧阳性对照的效果可能有较大的差别。如果在刺激后30分钟内观察不到活性氧的升高,可以适当提高活性氧阳性对照的浓度。如果活性氧升高得过快,可以适当降低活性氧阳性对照的浓度。Rosup对不同细胞的效果会有所不同。
另外,对于某些细胞,如果发现没有刺激的阴性对照细胞荧光也比较强,可以按照1:2000-1:5000稀释DCFH-DA,使装载探针时DCFH-DA的浓度为2-5微摩尔/升。
探针装载的时间也可以根据情况在15-60分钟内适当进行调整。
活性氧阳性对照(Rosup)仅仅用于作为阳性对照的样品,并不是在每个样品中都需加入活性氧阳性对照。

参考文献:
1.Rastogi RP, Singh SP, Häder DP, Sinha RP. Biochem Biophys Res Commun. 2010. 397(3):603-7.
2.Chen X, Zhong Z, Xu Z, Chen L, Wang Y. Free Radic Res. 2010. 44(6):587-604.

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EXP TOXICOL PATHOL 2011 Jan;63(1-2):69-78. (IF 2.023)
79. Liao Q, Li X, Zhou S, Liu L, Zhao S, Lian Y, Dong H.
J CARDIOVASC PHARM 2011 Feb;57(2):174-82. (IF 2.598)
80. Chang Y, Yang ST, Liu JH, Dong E, Wang Y, Cao A, Liu Y, Wang H.
Toxicol Lett 2011 Feb 5;200(3):201-10. (IF 3.569)
81. Li X, Ye X, Li X, Sun X, Liang Q, Tao L, Kang X, Chen J.
Brain Res 2011 Mar 25;1382:9-18. (IF 2.733)
82. Sun L, Guo C, Liu D, Zhao Y, Zhang Y, Song Z, Han H, Chen D, Zhao Y.
Neuroscience 2011 Jun 16;184:151-63. (IF 3.056)
84. Dong E, Wang Y, Yang ST, Yuan Y, Nie H, Chang Y, Wang L, Liu Y, Wang H.
J NANOSCI NANOTECHNO 2011 Sep;11(9):7848-56. (IF 1.134)
86. Li L, Lu N, Dai Q, Wei L, Zhao Q, Li Z, He Q, Dai Y, Guo Q.
Eur J Pharmacol 2011 Nov 16;670(1):13-21. (IF 3.263)
87. Zhang B, Liu ZY, Li YY, Luo Y, Liu ML, Dong HY, Wang YX, Liu Y, Zhao PT, Jin FG, Li ZC.
Eur J Pharm Sci 2011 Dec 18;44(5):573-9. (IF 3.616)
88. Khan M, Yu B, Rasul A, Al Shawi A, Yi F, Yang H, Ma T.
EVID-BASED COMPL ALT 2012;2012:703034. (IF 1.813)
89. Meng FM, Yang JB, Yang CH, Jiang Y, Zhou YF, Yu B, Yang H.
ASIAN PAC J CANCER P  2012;13(12):6369-74. (IF 1.24)
90. Cao G, Chen M, Song Q, Liu Y, Xie L, Han Y, Liu Z, Ji Y, Jiang Q.
Mol Med Rep 2012 Jan;5(1):54-9. (IF 2.1)
91. Guo C, He Z, Wen L, Zhu L, Lu Y, Deng S, Yang Y, Wei Q, Yuan H.
Cell Biol Int 2012 Feb;36(2):183-8. (IF 2.571)
92. Yu SJ, Liu HC, Ling-Ling E, Wang DS, Zhu GX.
EXP BIOL MED 2012 Apr;237(4):395-406. (IF 3.139)
93. Wang Y, Wei Y, Zhang H, Shi Y, Li Y, Li R.
Med Oncol 2012 Jun;29(2):1328-34. (IF 2.834)
94. Zhao D, Lin F, Wu X, Zhao Q, Zhao B, Lin P, Zhang Y, Yu X.
Toxicol In Vitro 2012 Jun;26(4):595-602. (IF 2.959)
95. Jia Y, Ji L, Zhang S, Xu L, Yin L, Li L, Zhao Y, Peng J.
Food Chem Toxicol 2012 Jul 4;50(9):3133-41. (IF 4.679)
98. Wu C, Shi L, Li Q, Jiang H, Selke M, Yan H, Wang X.
Nanomedicine 2012 Aug;8(6):860-9. (IF 4.717)
99. Zhang JK, Yang L, Meng GL, Fan J, Chen JZ, He QZ, Chen S, Fan JZ, Luo ZJ, Liu J.
Eur J Pharmacol 2012 Aug 15;689(1-3):31-7. (IF 3.263)
100. Li XY, Jing CQ, Lei WL, Li J, Wang JJ.
ECOTOX ENVIRON SAFE 2012 Sep;83:102-7. (IF 4.872)
101. Liu L, Fu J, Li T, Cui R, Ling J, Yu X, Ji H, Zhang Y.
Eur J Pharmacol 2012 Sep 15;691(1-3):61-8. (IF 3.263)
102. Zhang X, Liang D, Guo B, Yang L, Wang L, Ma J.
Biol Trace Elem Res 2012 Dec;150(1-3):424-32. (IF 2.639)
103. Zhang X, Wang J, Fan Y, Yang L, Wang L, Ma J.
Biol Trace Elem Res 2012 Dec;150(1-3):229-35. (IF 2.639)
104. Jiao L, Wan D, Zhang X, Li B, Zhao H, Liu S.
J Ethnopharmacol 2012 Dec 18;144(3):490-6. (IF 3.69)
105. Jiao K, Zhang J, Zhang M, Wei Y, Wu Y, Qiu ZY, He J, Cao Y, Hu J, Zhu H, Niu LN, Cao X, Yang K, Wang MQ.
PLoS One 2013;8(1):e53312. (IF 2.74)
106. Zhang JK, Yang L, Meng GL, Yuan Z, Fan J, Li D, Chen JZ, Shi TY, Hu HM, Wei BY, Luo ZJ, Liu J.
PLoS One 2013;8(2):e57251. (IF 2.74)
107. Ge Y, Weng X, Tian T, Ding F, Huang R, Yuan L, Wu J, Wang T, Guo P, Zhou X.
RSC Adv 2013;3:12839-46. (IF 3.119)
109. Cheng P, Ni Z, Dai X, Wang B, Ding W, Rae Smith A, Xu L, Wu D, He F, Lian J.
Cell Death Dis 2013 Feb 7;4:e489. (IF 6.304)
110. Cui S, Chen H, Qian Z, Chen W, Gu Y.
Biophotonics and Immune Responses VIII 2013 Feb 21; 85820A.
112. Du HF, Yu LJ, Meng YF, Lv HY, Meng J, Song XN, Zhang JQ.
LEUKEMIA LYMPHOMA 2013 Mar;54(3):607-18. (IF 2.969)
113. Wang K, Gao F, Ji Y, Liu Y, Dan Z, Yang P, Zhu Y, Li S.
New Phytol 2013 Apr;198(2):408-18. (IF 8.512)
114. Li XH, Chen XJ, Ou WB, Zhang Q, Lv ZR, Zhan Y, Ma L, Huang T, Yan YB, Zhou HM.
INT J BIOCHEM CELL B 2013 May;45(5):979-86. (IF 3.673)
115. Qian C, Wang JQ, Song CL, Wang LL, Ji LN, Chao H.
Metallomics 2013 Jun 27;5(7):844-54. (IF 3.796)
116. Chen G, Zhang P, Huang T, Yu W, Lin J, Li P, Chen K.
Carbohydrate Polymers 2013 Sep 12; 97(2):800-8. (IF 6.044)
117. Ni Z, Dai X, Wang B, Ding W, Cheng P, Xu L, Lian J, He F.
LEUKEMIA LYMPHOMA 2013 Oct;54(10):2263-8. (IF 2.969)
118. Liu Y, Dai C, Gao R, Li J.
TOXICOL MECH METHOD 2013 Oct;23(8):584-90. (IF 2.295)
119. Wang A, Pu K, Dong B, Liu Y, Zhang L, Zhang Z, Duan W, Zhu Y.
J Appl Toxicol 2013 Oct;33(10):1156-64. (IF 2.997)
120. Zhong X, Lin R, Li Z, Mao J, Chen L.
Biol Pharm Bull 2014;37(7):1199-206. (IF 1.863)
121. Cheng Y, Feng Y, Zhu M, Yan B, Fu S, Guo J, Hu J, Song X, Guo S, Liu G.
Acta Histochem 2014 Jan;116(1):214-21. (IF 2.107)
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Mol Cell Biochem 2014 Feb;387(1-2):261-70. (IF 2.795)
123. Wan X, Meng J, Dai Y, Zhang Y, Yan S.
PLoS One 2014 Feb 5;9(2):e88137. (IF 2.74)
124. Zheng S, Liu J, Han Q, Huang S, Su W, Fu J, Jia X, Du S, Zhou Y, Zhang X, Guan Y.
J Diabetes 2014 Mar;6(2):132-46. (IF 3.28)
125. He X, Song W, Liu C, Chen S, Hua J.
CELL PROLIFERAT 2014 Apr;47(2):161-71. (IF 5.753)
126. Pan B, Yang L, Wang J, Wang Y, Wang J, Zhou X, Yin X, Zhang Z, Zhao D.
Mol Neurobiol 2014 Apr;49(2):1102-16. (IF 4.5)
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APPL MICROBIOL BIOT 2014 Apr;98(7):3099-110. (IF 3.53)
128. Xu J, Meng K, Zhang R, Yang H, Liao C, Zhu W, Jiao J.
PLoS One 2014 May 15;9(5):e97906. (IF 2.74)
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EUR J PHARM SCI 2014 May;103(5):1529-37. (IF 3.616)
130. Zhao J, Wu Y, Alfred AT, Wei P, Yang S.
Lett Appl Microbiol 2014 Jun;58(6):541-8. (IF 2.173)
131. Zhong L, Wang Y, Li W, Gu J, Li X, Wang X, Yue Z, Mu Y, Bai J, Li R, Zhang H.
Mol Cell Biochem 2014 Jul;392(1-2):135-44. (IF 2.795)
132. Xu SY, Hu YF, Li WP, Wu YM, Ji Z, Wang SN, Li K, Pan SY.
Int J Biol Sci 2014 Jul 29;10(8):873-81. (IF 4.858)
133. Shen C, Wang S, Shan Y, Liu Z, Fan F, Tao L, Liu Y, Zhou L, Pei C, Wu H, Tian C, RuanJ, Chen W, Wang A, Zheng S, Lu Y.
Food Funct 2014 Jul 25;5(7):1422-31. (IF 4.171)
134. Yuan D, Wan JZ, Deng LL, Zhang CC, Dun YY, Dai YW, Zhou ZY, Liu CQ, Wang T.
Int J Mol Sci 2014 Jul 28;15(8):13209-22. (IF 4.556)
135. Zhang J, Xu L, Zhang L, Ying Z, Su W, Wang T.
J Nutr 2014 Aug;144(8):1211-8. (IF 4.281)
136. Jian Z, Li K, Song P, Zhu G, Zhu L, Cui T, Liu B, Tang L, Wang X, Wang G, Gao T, Li C.
J Invest Dermatol 2014 Aug;134(8):2221-30. (IF 7.143)
137. Yang X, He C, Li J, Chen H, Ma Q, Sui X, Tian S, Ying M, Zhang Q, Luo Y, Zhuang Z, LiuJ.
Toxicol Lett 2014 Aug 17;229(1):240-9. (IF 3.569)
138. Miao SH, Sun HB, Ye Y, Yang JJ, Shi YW, Lu M, Hu G, Zhou JW.
CNS Neurosci Ther 2014 Aug;20(8):754-62. (IF 4.074)
139. Ma W, Yuan L, Yu H, Xi Y, Xiao R.
Life Sci 2014 Aug 21;110(2):53-60. (IF 3.647)
140. Xu T, Pang Q, Zhou D, Zhang A, Luo S, Wang Y, Yan X.
PLoS One 2014 Aug 22;9(8):e105768. (IF 2.74)
141. Ma Y, Li R, Zhang Y, Zhou L, Dai Y.
FREE RADICAL BIO MED 2014 Sep 16;76C:251-260. (IF 6.17)
142. Zhao JY, Cui R, Zhang ZL, Zhang M, Xie ZX, Pang DW.
Nanoscale 2014 Oct 9;6(21):13126-34. (IF 6.895)
143. Jin Y, Liu K, Peng J, Wang C, Kang L, Chang N, Sun H.
Toxicol Lett 2014 Oct 8; 232(1):149-158. (IF 3.569)
144. Xie J, Bai X, Li Y, Sun C, Qian H, Fu Z.
Ecotoxicology 2014 Oct;23(8):1430-8. (IF 2.535)
145. Chen Y, Zhao S, Wang Y, Li Y, Bai L, Liu R, Fan J, Liu E.
Int J Mol Med 2014 Nov;34(5):1277-85. (IF 3.098)
146. Wang S, Shi N, Geng Z, Li X, Hu X, Wang Z.
Biochimie 2014 Nov;106:167-74. (IF 3.413)
147. Guan G, Han H, Yang Y, Jin Y, Wang X, Liu X.
Endocrine 2014 Dec;47(3):764-71. (IF 3.235)
148. Gu M, Xu J, Han C, Kang Y, Liu T, He Y, Huang Y, Liu C.
EVID-BASED COMPL ALT 2015;2015:796306. (IF 1.813)
149. Xiong L, Xie J, Song C, Liu J, Zheng J, Liu C, Zhang X, Li P, Wang F.
EVID-BASED COMPL ALT 2015;2015:187265. (IF 1.813)
150. Li Y, Chen K, Zhou Y, Xiao Y, Deng M, Jiang Z, Ye W, Wang X, Wei X, Li J, Liang J, Zheng Z, Yao Y, Wang W, Li P, Xu B.
CURR CANCER DRUG TAR 2015;15(6):493-503. (IF 2.912)
151. Xiao W, Jiang Y, Men Q, Yuan L, Huang Z, Liu T, Li W, Liu X.
Int J Oncol 2015 Jan;46(1):360-8. (IF 3.899)
152. Xing Y, Bao W, Fan X, Liu K, Li X, Xi T.
Apoptosis 2015 Jan;20(1):83-91. (IF 4.543)
154. Lan QY, Liu QL, Cai J, Liu AW.
INT J CLIN EXP PATHO 2015 Jan 1;8(1):155-63. (IF 0.252)
155. Wang X, Yu S, Wang CY, Wang Y, Liu HX, Cui Y, Zhang LD.
IN VITRO CELL DEV-AN 2015 Feb;51(2):204-9. (IF 1.665)
156. Bai YH, Wang JP, Yang M, Zeng Y, Jiang HY.
BIOCHEM BIOPH RES CO 2015 Feb 20;457(4):730-5. (IF 2.985)
157. Shi J, Sun B, Shi W, Zuo H, Cui D, Ni L, Chen J.
TUMOR BIOL  2015 Feb;36(2):655-62. (IF 3.65)
158. Jiang L, Wang L, Chen L, Cai GH, Ren QY, Chen JZ, Shi HJ, Xie YH.
Int J Clin Exp Med 2015 Feb 15;8(2):2190-6. (IF 0.166)
159. Zhan R, Han Q, Zhang C, Tian Z, Zhang J.
Infect Immun 2015 Apr;83(4):1641-9. (IF 3.201)
160. Zhang Q, Cheng G, Qiu H, Zhu L, Ren Z, Zhao W, Zhang T, Liu L.
Food Funct 2015 May;6(5):1518-25. (IF 4.171)
161. Hua P, Sun M, Zhang G, Zhang Y, Tian X, Li X, Cui R, Zhang X.
BIOCHEM BIOPH RES CO 2015 May 1;460(2):136-42. (IF 2.985)
163. Yu S, Hou D, Chen P, Zhang Q, Lv B, Ma Y, Liu F, Liu H, Song EJ, Yang D, Liu J.
BIOCHEM BIOPH RES CO 2015 May 8;460(3):759-65. (IF 2.985)
164. Hou W, Gao W, Wang D, Liu Q, Zheng S, Wang Y.
PLoS One 2015 May 15;10(5):e0127177. (IF 2.74)
165. Chen P, Hu YF, Wang L, Xiao WF, Bao XY, Pan C, Yi HS, Chen XY, Pan MH, Lu C.
PLoS One 2015 Jul 30;10(7):e0134694. (IF 2.74)
166. Yan X, Yang X, Hao X, Ren Q, Gao J, Wang Y, Chang N, Qiu Y, Song G.
Biol Trace Elem Res 2015 Aug;166(2):210-5. (IF 2.639)
167. Zhang F, Cui J, Lv B, Yu B.
Int J Mol Med 2015 Aug;36(2):415-23. (IF 3.098)
169. Wei X, Zhu X, Hu N, Zhang X, Sun T, Xu J, Bian X.
BIOCHEM BIOPH RES CO 2015 Sep 11;465(1):101-7. (IF 2.985)
170. Qiu M, Chen L, Tan G, Ke L, Zhang S, Chen H, Liu J.
SCI REP-UK 2015 Oct 13;5:15104. (IF 3.998)
171. Li Y, Zhu H, Wang S, Qian X, Fan J, Wang Z, Song P, Zhang X, Lu W, Ju D.
Theranostics 2015 Oct 8;5(12):1363-77. (IF 8.579)
172. Song B, Zhang H, Jiang L, Chi Y, Tian J, Du W, Yu B, Han Z.
ACTA BIOCH BIOPH SIN 2015 Oct;47(10):805-14. (IF 2.836)
173. Xue H, Gan F, Zhang Z, Hu J, Chen X, Huang K.
Int J Biol Macromol 2015 Nov;81:22-30. (IF 5.162)
174. Zhang E, Guo Q, Gao H, Xu R, Teng S, Wu Y.
PLoS One 2015 Dec 2;10(12):e0143814. (IF 2.74)
175. Shen K, Wang Y, Zhang Y, Zhou H, Song Y, Cao Z, Kou J, Yu B.
REJUV RES 2015 Dec;18(6):517-27. (IF 3.811)
176. Hong Z, Guo Z, Zhang R, Xu J, Dong W, Zhuang G, Deng C.
Tohoku J Exp Med 2016;239(2):117-25. (IF 1.441)
177. Feng B, Qiu L, Ye C, Chen L, Fu Y, Sun W.
Int J Radiat Biol 2016;92(3):148-55. (IF 2.368)
178. Tian X, Zhao L, Song X, Yan Y, Liu N, Li T, Yan B, Liu B.
Biomed Res Int 2016;2016:4847874. (IF 2.276)
179. Zhang M, Du Y, Lu R, Shu Y, Zhao W, Li Z, Zhang Y, Liu R, Yang T, Luo S, Gao M, Zhang Y, Zhang G, Liu J, Lu Y.
Oxid Med Cell Longev 2016;2016:7524308. (IF 5.076)
180. Cao LJ, Li HD, Yan M, Li ZH, Gong H, Jiang P, Deng Y, Fang PF, Zhang BK.
EVID-BASED COMPL ALT 2016;2016:8912184. (IF 1.813)
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Biomed Res Int 2016;2016:2581061. (IF 2.276)
182. Yang F, Yan G, Li Y, Han Z, Zhang L, Chen S, Feng C, Huang Q, Ding F, Yu Y, Bi C, Cai B, Yang L.
CELL PHYSIOL BIOCHEM 2016;39(4):1369-79. (IF 5.5)
183. Wei C, Wang Y, Li M, Li H, Lu X, Shao H, Xu C.
CELL PHYSIOL BIOCHEM 2016;38(2):531-44. (IF 5.5)
184. Yu W, Chen G, Zhang P, Chen K.
Int J Biol Macromol 2016 Jan;82:299-307. (IF 5.162)
185. Qin M, Lin Z, Wang D, Long X, Zheng M, Qiu Y.
ENVIRON TOXICOL CHEM 2016 Jan;41:251-8. (IF 3.152)
186. Zeng L, Chen Y, Liu J, Huang H, Guan R, Ji L, Chao H.
SCI REP-UK 2016 Jan 14;6:19449. (IF 3.998)
187. Chen C, Cao J, Ma X, Wang X, Chen Q, Yan S, Zhao N, Geng Z, Wang Z.
SCI REP-UK 2016 Feb 9;6:20853. (IF 3.998)
189. Li B, Zhang J, Wang Z, Chen S.
PLoS One 2016 Feb 18;11(2):e0149694. (IF 2.74)
190. Kong GM, Tao WH, Diao YL, Fang PH, Wang JJ, Bo P, Qian F.
WORLD J GASTROENTERO 2016 Mar 21;22(11):3186-95. (IF 3.665)
191. Xie D, Wu X, Lan L, Shangguan F, Lin X, Chen F, Xu S, Zhang Y, Chen Z, Huang K, Wang R, Wang L, Song X, Liu Y, Lu B.
ONCOTARGET 2016 Mar 8;7(10):11609-24. (IF 5.168)
192. Zhang X, Wang L, Wang R, Luo X, Li Y, Chen Z.
Food Funct 2016 Mar;7(3):1429-37. (IF 4.171)
193. Shu Y, Liu Y, Li X, Cao L, Yuan X, Li W, Cao Q.
BIOMOL THER 2016 Mar 1;24(2):132-9. (IF 3.47)
194. Chen H, Lu Y, Cao Z, Ma Q, Pi H, Fang Y, Yu Z, Hu H, Zhou Z.
Toxicol Lett 2016 Mar 30;246:7-16. (IF 3.569)
195. Zhang X, Wang Y, Han S, Xiang H, Peng Y, Wu Y, Pan S, Zhang Y, Ruan J.
PLoS One 2016 Mar 14;11(3):e0151679. (IF 2.74)
196. Sun A, Lin J, Pi C, Xu R, Cui X.
ARCH PHARM 2016 Mar;349(3):186-92. (IF 2.59)
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Int J Mol Med 2016 Apr;37(4):949-57. (IF 3.098)
198. Shang CH, Zhang QQ, Zhou JH.
Inflammation 2016 Apr;39(2):873-80. (IF 3.212)
199. Liu Z, Zhan X, Yang M, Yang Q, Xu X, Lan F, Wu Y, Gu Z.
Nanoscale 2016 Apr 14;8(14):7544-55. (IF 6.895)
200. Chen Y, Zhang Y, Ji H, Ji Y, Yang J, Huang J, Sun D.
Toxicol In Vitro 2016 Apr;32:146-53. (IF 2.959)
201. Zhang J, Jiang H, Xie L, Hu J, Li L, Yang M, Cheng L, Liu B, Qian X.
ONCOTARGETS THER 2016 May 24;9:2885-95. (IF 3.337)
202. Wang Y, Zhang S, Zhang C, Zhao Z, Zheng X, Xue L, Liu J, Yuan XC.
Analyst 2016 May 23;141(11):3411-20. (IF 3.978)
203. Zhang T, Shao Y, Chu TY, Huang HS, Liou YL, Li Q, Zhou H.
Am J Cancer Res 2016 May 1;6(5):957-72. (IF 5.177)
204. Lu MC, Ji JA, Jiang YL, Chen ZY, Yuan ZW, You QD, Jiang ZY.
SCI REP-UK 2016 May 24;6:26585. (IF 3.998)
205. Zhu W, Ma L, Yang B, Zheng Z, Chai R, Liu T, Liu Z, Song T, Li F, Li G.
IN VITRO CELL DEV-AN 2016 May;52(5):589-97. (IF 1.665)
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ONCOTARGET 2016 May 17;7(20):29102-15. (IF 5.168)
207. Liu G, Kuang S, Wu S, Jin W, Sun C.
SCI REP-UK 2016 May 24;6:26722. (IF 3.998)
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Mol Neurobiol 2016 May 6.[Epub ahead of print] (IF 4.5)
209. Qiao ZY, Zhao WJ, Cong Y, Zhang D, Hu Z, Duan ZY, Wang H.
Biomacromolecules 2016 May 9;17(5):1643-52. (IF 6.092)
210. Zhou XY, Zhang T, Ren L, Wu JJ, Wang W, Liu JX.
Aquat Toxicol 2016 Jun;175:1-11. (IF 4.344)
211. Yin T, Zhang Z, Cao B, Duan Q, Shi P, Zhao H, Camara SN, Shen Q, Wang C.
ONCOTARGET 2016 Jun 14;7(24):37192-37204. (IF 5.168)
212. Qi L, Lv X, Zhang T, Jia P, Yan R, Li S, Zou R, Xue Y, Dai L.
SCI REP-UK 2016 Jun 1;6:26961. (IF 3.998)
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ONCOTARGET 2016 Jun 14;7(24):35618-35631. (IF 5.168)
214. Xu C, Liu Q, Liu H, Zhang C, Shao W, Gu A.
ONCOTARGET 2016 Jun 28;7(26):39270-39278. (IF 5.168)
215. Li X, Song Y, Zhang P, Zhu H, Chen L, Xiao Y, Xing Y.
TUMOR BIOL  2016 Jun;37(6):7599-613. (IF 3.65)
217. Wang H, Liang X, Luo G, Ding M, Liang Q.
Mol Biosyst 2016 Jun 21;12(7):2257-64. (IF 3.336)
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SCI REP-UK 2016 Jun 27;6:28734. (IF 3.998)
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SCI REP-UK 2016 Jun 16;6:27983. (IF 3.998)
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SCI REP-UK 2016 Jun 28;6:28701. (IF 3.998)
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Biomed Pharmacother 2016 Jul;81:63-70. (IF 4.545)
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Acta Pharmacol Sin 2016 Jul;37(7):950-62. (IF 5.064)
223. Cheong CU, Yeh CS, Hsieh YW, Lee YR, Lin MY, Chen CY, Lee CH.
Molecules 2016 Jul 9;21(7). pii: E898. (IF 3.267)
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BIOMOL THER 2016 Jul 1;24(4):371-9. (IF 3.47)
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J ANTIMICROB CHEMOTH 2016 Aug;71(8):2192-9. (IF 5.439)
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Inflammation 2016 Aug;39(4):1421-33. (IF 3.212)
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Int J Biol Macromol 2016 Aug;89:376-88. (IF 5.162)
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INT J BIOCHEM CELL B 2016 Aug;77(Pt A):80-90. (IF 3.673)
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Theranostics 2016 Aug 8;6(11):1899-917. (IF 8.579)
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J Oral Pathol Med 2016 Sep 22.doi: 10.1111/jop.12502. [Epub ahead of print] (IF 2.495)
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Acta Pharmacol Sin 2016 Sep;37(9):1178-89. (IF 5.064)
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J BIOCHEM MOL TOXIC 2016 Sep;30(9):462-9. (IF 3.606)
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J Ginseng Res 2016 Oct;40(4):351-358. (IF 5.487)
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J Nutr Biochem 2016 Oct;36:81-88. (IF 4.873)
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J Cell Mol Med 2016 Oct;20(10):1861-71. (IF 4.486)
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Neurotox Res 2016 Oct;30(3):486-98. (IF 2.992)
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Mol Med Rep 2016 Nov;14(5):4063-4068. (IF 2.1)
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