6X His Peptide (6X His多肽),也称6X His-tag Peptide (6X His标签多肽)、6X His tag Peptide (6X His tag多肽)或His-tag Peptide (His标签多肽),是一种常用于通过竞争性结合Anti-His抗体(也称His-tag antibody、His标签抗体、6X His antibody或6X His抗体),从而在免疫沉淀时洗脱与Anti-His抗体结合的6X His融合表达蛋白的多肽。本产品不仅可以用于Anti-His Magnetic Beads (Anti-His磁珠) (P2121)免疫沉淀时6X His融合表达蛋白的洗脱,也可以用于常规的Anti-His抗体免疫沉淀时6X His融合表达蛋白的洗脱。
本产品竞争性洗脱6X His融合表达蛋白后,洗脱后的样品中不含Anti-His抗体的轻链和重链,可以有效解决免疫沉淀后Western检测时轻链和重链的干扰问题。普通的免疫沉淀,通常是通过加入上样缓冲液煮沸从而使His融合表达蛋白进入到溶液中,但此时Anti-His抗体的轻链和重链也会进入到溶液中,并很可能会干扰后续的Western检测。
6X His Peptide (6X His多肽)是合成的多肽,其氨基酸序列为His-His-His-His-His-His (HHHHHH)。
6X His Peptide (6X His多肽)的主要参数如下:
About this protein | |
Product name | 6X His Peptide (6X His多肽) |
Alternative names | His Tag; His Synthetic Peptide; His Tag Peptide; Hexa-histidine; Hexa His Tag Peptide |
Description | 6X His Peptide is a synthetic peptide with a molecular weight of 840.85. The amino acid sequence is His-His-His-His-His-His. |
Purity | ~98% by HPLC |
Molecular Formula | C36H44N18O7 |
Molecular Weight | ~840.85 |
Sequence (Three-Letter Code) | His-His-His-His-His-His |
Sequence (One-Letter Code) | HHHHHH |
Source | Synthetic |
Reconstitution | TBS (50mM Tris-HCl, pH7.4, 150mM NaCl) |
本产品5mg/ml包装产品配制在TBS中,可以直接使用。25mg为粉末装。6X His Peptide (6X His多肽)常用储存液浓度为5-25mg/ml,常用使用浓度为100-400μg/ml。
包装清单:产品编号 | 产品名称 | 包装 |
P9811-1mg | 6X His Peptide (6X His多肽) | 5mg/ml×0.2ml |
P9811-5mg | 6X His Peptide (6X His多肽) | 5mg/ml×1ml |
P9811-25mg | 6X His Peptide (6X His多肽) | 25mg |
- | 说明书 | 1份 |
-20℃保存,至少一年有效。P9811-25mg粉末装也可以4℃保存,一年有效。
注意事项:由于多肽的每次冻融均会引起部分失活,所以溶液装或粉末装配制成溶液后须分装后于-20℃或更低温度冻存,以避免反复冻融。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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