Species | Gene ID | Accession | Source | Length | MW | Tag |
Vibrio cholerae | 2613962 | P01556 | E. coli | 103aa | 11.6kDa | - |
About this Protein | |
Name | (Recombinant Cholera Toxin B subunit; rCTB);重组霍乱毒素B亚基 |
Synonyms | CTB; Choleragen; CTX; Ctx; CT |
Purity | >98% by SDS-PAGE and HPLC analyses. |
Biological Activity | Data Not Available. |
Physical Appearance | Sterile colorless liquid. |
Formulation | Supplied as a 0.2µM filtered solution in 5mM PB, pH7.0, 75mM NaCl, with 50% glycerol. |
Endotoxin | Less than 0.1EU/µg of rCTB as determined by LAL method. |
Reconstitution | - |
Category | Others |
Background | Cholera toxin is protein complex secreted by the bacterium Vibrio cholerae. CTX is responsible for the massive, watery diarrhea characteristic of cholera infection. The cholera toxin is an oligomeric complex made up of six protein subunits: a single copy of the A subunit (part A, enzymatic), and five copies of the B subunit (part B, receptor binding), denoted as AB5. Subunit B binds while subunit A activates the G protein which activates adenylate cyclase. The five B subunits - each weighing 11 kDa, form a five-membered ring. The A subunit which is 28 kDa, has two important segments. The A1 portion of the chain (CTA1) is a globular enzyme payload that ADP-ribosylates G proteins, while the A2 chain (CTA2) forms an extended alpha helix which sits snugly in the central pore of the B subunit ring. This structure is similar in shape, mechanism, and sequence to the heat-labile enterotoxin secreted by some strains of the Escherichia coli bacterium. |
Amino Acid Sequence | TPQNITDLCA EYHNTQIYTL NDKIFSYTES LAGKREMAII TFKNGAIFQV EVPGSQHIDS QKKAIERMKD TLRIAYLTEA KVEKLCVWNN KTPHAIAAIS MAN |
包装清单:
产品编号 | 产品名称 | 包装 |
P7418-100µg | Recombinant Cholera Toxin B subunit | 100µg |
P7418-500µg | Recombinant Cholera Toxin B subunit | 500µg |
P7418-1mg | Recombinant Cholera Toxin B subunit | 1mg |
- | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
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