| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 3958 | P17931 | E. coli | 249aa | 26kDa | － |
| About this Antibody | |
| Name | Recombinant Human Galectin-3 (Recombinant Human Galectin-3; rHuGalectin-3)；重组人半乳糖凝集素3 |
| Synonyms | AGE-R3; CBP35; GAL3; L29; LGALS3; Mac-2; Carbohydrate-binding protein 35; CBP 35,35 kDa lectin; Gal-3; galactin-3; Galactose-specific lectin 3; Galactoside-binding protein; GALBPCBP35; galectin-3; GALIG; IgE-binding protein; L31; L-31; Laminin-binding protein; Lectin L-29; lectin, galactoside-binding, soluble, 3; LGALS2; MAC-2; Mac-2 antigen; MAC2GAL3 |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by its ability to agglutinate human red blood cells is less than 10μg/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered solution in 1X PBS, pH7.4, 3mM DTT. |
| Endotoxin | Less than 0.1EU/μg of rHuGalectin-3 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Others |
| Background | Human Galectin-3 also named AGE-R3, CBP35, GAL3, L29, LGALS3, Mac-2, is belonging to the galectins family and it is encoded by a single gene, LGALS3, located on chromosome 14, locus q21–q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Galectin-3 is approximately 30kDa and, like all galectins, contains a carbohydrate-recognition-binding domain (CRD) of about 130 amino acids that enable the specific binding of β-galactosides. Given Galectin-3's broad biological functionality, it has been demonstrated to be involved in cancer, inflammation and fibrosis, heart disease, and stroke. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Human Galectin-3 shares 79% amino acid sequence identity with rat and mouse Galectin-3, respectively. |
| Amino Acid Sequence | ADNFSLHDAL SGSGNPNPQG WPGAWGNQPA GAGGYPGASY PGAYPGQAPP GAYPGQAPPG AYPGAPGAYP GAPAPGVYPG PPSGPGAYPS SGQPSATGAY PATGPYGAPA GPLIVPYNLP LPGGVVPRML ITILGTVKPN ANRIALDFQR GNDVAFHFNP RFNENNRRVI VCNTKLDNNW GREERQSVFP FESGKPFKIQ VLVEPDHFKV AVNDAHLLQY NHRVKKLNEI SKLGISGDID LTSASYTMI |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P7302-10μg | Recombinant Human Galectin-3 | 10μg |
| P7302-50μg | Recombinant Human Galectin-3 | 50μg |
| P7302-100μg | Recombinant Human Galectin-3 | 100μg |
| P7302-1mg | Recombinant Human Galectin-3 | 1mg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
