| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 335 | P02647 | E. coli | 243aa | 28.1kDa | － |
| About this protein | |
| Name | Recombinant Human ApoA-I (Recombinant Human Apolipoprotein A-I; rHuApoA-I)；重组人载脂蛋白A-I |
| Synonyms | Alp-1; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; APOA1; Apo-AI; apo-AI; ApoA-I; apoA-I; Apolipoprotein A1; apolipoprotein A-I; MGC117399 |
| Purity | >98% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data Not Available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μM filtered concentrated solution in PBS, pH7.4. |
| Endotoxin | Less than 0.1EU/μg of rHuApoA-I as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Others |
| Background | Apolipoprotein A-I encoded by the APOA1 gene is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Apolipoprotein A-I participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). Apolipoprotein A-I is also isolated as a prostacyclin (PGI2) stabilizing factor and thus may have an anticlotting effect. Apolipoprotein A-I has been shown to interact with ABCA1, GPLD1 and PLTP. Apolipoprotein A-I binds to lipopolysaccharide or endotoxin and has a major role in the anti-endotoxin function of HDL. Mature human ApoA-1 shares 65% and 62%a.a. sequence identity with murine and rat ApoA-1, respectively. It has an approximate molecular weight of 28.1kDa. |
| Amino Acid Sequence | DEPPQSPWDR VKDLATVYVD VLKDSGRDYV SQFEGSALGK QLNLKLLDNW DSVTSTFSKL REQLGPVTQE FWDNLEKETE GLRQEMSKDL EEVKAKVQPY LDDFQKKWQE EMELYRQKVE PLRAELQEGA RQKLHELQEK LSPLGEEMRD RARAHVDALR THLAPYSDEL RQRLAARLEA LKENGGARLA EYHAKATEHL STLSEKAKPA LEDLRQGLLP VLESFKVSFL SALEEYTKKL NTQ |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P7280-20μg | Recombinant Human ApoA-I | 20μg |
| P7280-100μg | Recombinant Human ApoA-I | 100μg |
| P7280-1mg | Recombinant Human ApoA-I | 1mg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
