| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 6657 | P48431 | E. coli | 330aa | 36kDa | － |
| About this Antibody | |
| Name | Recombinant Human SOX2-TAT (Recombinant Human SOX2-TAT; rHuSOX2-TAT)；重组人SOX2蛋白，TAT标签 |
| Synonyms | ANOP3; MCOPS3; MGC2413; SRY (sex determining region Y)-box 2; SRY-related HMG-box gene 2; transcription factor SOX2; transcription factor SOX-2 |
| Purity | >95% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Data Not Available. |
| Physical Appearance | Sterile Filtered lyophilized (freeze-dried) liquid-like powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, with 5% Trehalose. |
| Endotoxin | Less than 1EU/μg of rHuSOX2-TAT as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Others |
| Background | Sox2, also named SRY (sex determining region Y)-box 2, is belonging to the Sox family and it is encoded by the Sox gene in human. This protein family shares highly conserved DNA binding domains known as HMG (High-mobility group) box domains containing approximately 80 amino acids. Sox2 plays a role in maintenance of embryonic and neural stem cells and holds great promise in research involving induced pluripotency, an emerging and very promising field of regenerative medicine. Mature human Sox2 shares 100 amino acid sequence identity with murine and rat Sox2. Recombinant human Sox2-TAT expressed in E. coli is a 36kDa protein containing 330 amino-acid residues, including the 317 residues of full-length Sox2 and a 13-residue Cterminal TAT peptide (GGYGRKKRRQRRR). |
| Amino Acid Sequence | MYNMMETELK PPGPQQTSGG GGGNSTAAAA GGNQKNSPDR VKRPMNAFMV WSRGQRRKMA QENPKMHNSE ISKRLGAEWK LLSETEKRPF IDEAKRLRAL HMKEHPDYKY RPRRKTKTLM KKDKYTLPGG LLAPGGNSMA SGVGVGAGLG AGVNQRMDSY AHMNGWSNGS YSMMQDQLGY PQHPGLNAHG AAQMQPMHRY DVSALQYNSM TSSQTYMNGS PTYSMSYSQQ GTPGMALGSM GSVVKSEASS SPPVVTSSSH SRAPCQAGDL RDMISMYLPG AEVPEPAAPS RLHMSQHYQS GPVPGTAING TLPLSHMGGY GRKKRRQRRR |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P7235-5μg | Recombinant Human SOX2-TAT | 5μg |
| P7235-100μg | Recombinant Human SOX2-TAT | 100μg |
| P7235-500μg | Recombinant Human SOX2-TAT | 500μg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
