Recombinant Human ME2, His-tag
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产品编号 P7014-500μg
数量
¥ 12990.00
产品包装:
500μg 5μg 100μg 1mg
产品简介
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Species Gene ID Accession Source Length MW Tag
Human 4200 P23368 E. coli 573aa 64.4kDa His
About this Antibody
Name Recombinant Human ME2, His-tag (Recombinant Human Malic Enzyme 2, His-tag; rHuME2, His-tag);重组人苹果酸酶2,His标签
Synonyms NAD-dependent malic enzyme, mitochondrial; NAD-ME; Malic enzyme 2; ME2
Purity >95% by SDS-PAGE and HPLC analyses.
Biological Activity Malic Enzyme activity was assayed spectrophotometrically at 340nm as described in Mandela and Sauer (1975). The standard reaction mixture contained 50mM Tris-HCl, 3mM MnCl2, 5mM malate, 0.12mM NADP+, 2.5mM fumarate. Assay was performed in a Beckman spectrophotometer. The Km value is 1.5±0.6mM.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4.
Endotoxin Less than 1EU/μg of rHuME2, His-tag as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Enzymes
Background NAD-dependent malic enzyme (ME2), mitochondrial is a protein that in humans is encoded by the ME2 gene. This gene encodes a mitochondrial NAD-dependent malic enzyme, a homotetrameric protein, which catalyzes the oxidative decarboxylation of malate to pyruvate. Three different isoforms of ME are known to be in mammalian tissues: a strictly cytosolic NADP+-dependent enzyme, an NADP+-dependent mitochondriail isoform, and a mitochondrial isoenzyme that is able to use both NAD+ and NADP+ but is more effective with NAD+. The mammalian isoforms size is about 62-64kDa. A native size of 240,000 Da proposes a tetrameric structure for the active enzyme.
Amino Acid Sequence MLHIKEKGKP LMLNPRTNKG MAFTLQERQM LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RNTLIQFEDF GNHNAFRFLR KYREKYCTFN DDIQGTAAVA LAGLLAAQKV ISKPISEHKI LFLGAGEAAL GIANLIVMSM VENGLSEQEA QKKIWMFDKY GLLVKGRKAK IDSYQEPFTH SAPESIPDTF EDAVNILKPS TIIGVAGAGR LFTPDVIRAM ASINERPVIF ALSNPTAQAE CTAEEAYTLT EGRCLFASGS PFGPVKLTDG RVFTPGQGNN VYIFPGVALA VILCNTRHIS DSVFLEAAKA LTSQLTDEEL AQGRLYPPLA NIQEVSINIA IKVTEYLYAN KMAFRYPEPE DKAKYVKERT WRSEYDSLLP DVYEWPESAS SPPVITEHHH HHH

包装清单:

产品编号 产品名称 包装
P7014-5μg Recombinant Human ME2, His-tag 5μg
P7014-100μg Recombinant Human ME2, His-tag 100μg
P7014-500μg Recombinant Human ME2, His-tag 500μg
- 说明书 1份

保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。
2. 将本产品和自行准备的产品简介中Reconstitution一栏推荐的配制储存液平衡至室温。
3. 除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开盖子前,我们建议在离心机中约8,000-12,000×g离心10-30秒,使附着在盖子或容器壁上的蛋白聚集于容器底部。
4. 加入适量推荐的配制储存液,达到Reconstitution一栏中推荐的浓度。请勿用vortex剧烈振荡或移液器剧烈吹打,以免蛋白变性而失活。
5. 在室温下溶解15-30分钟。
6. 如果看不到蛋白粉末,并担心蛋白仍然粘附在盖子或容器壁上,可以吸取液体轻轻润洗盖子和容器壁以促进溶解。对于溶解的体积相对比较大的情况,可使用BeyoVortex™基础型旋转混匀仪(E6800)或其它适当摇床或手动轻轻上下颠倒以促进溶解。
7. 如需分装,每管体积通常不小于10μl,并按说明书中推荐的储存液保存条件进行保存。
8. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。
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