Recombinant Rat MIP-1α/CCL3
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产品编号 P6906-1mg
数量
¥ 19650.00
产品包装:
5μg 100μg 500μg 1mg
产品简介
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Species Gene ID Accession Source Length MW Tag
Rat 25542 P50229 E. coli 69aa 7.9kDa
About this Antibody
Name Recombinant Rat MIP-1α/CCL3 (Recombinant Rat Macrophage Inflammatory Protein-1α/CCL3; rRtMIP-1α/CCL3);重组大鼠巨噬细胞炎症蛋白1α/CCL3
Synonyms LD78a; MIP-1 alpha; C-C motif chemokine 3; MIP1-(a); AI323804; chemokine (C-C motif) ligand 3; G0S19-1; LD78alpha; Mip1a; MIP-1alpha; MIP1-alpha; Scya3
Purity >98% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemoattract bioassay using human blood monocytes is in a concentration range of 10-100ng/ml.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2μm filtered concentrated solution in 30% Acetonitrile and 0.1% TFA.
Endotoxin Less than 0.1EU/μg of rRtMIP-1α/CCL3 as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Chemokines
Background CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant rat MIP-1α is a 7.9kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and mature rat CCL3 shares 74%, 91% and 88% amino acid sequence identity with human, mouse and cotton rat CCL3, respectively.
Amino Acid Sequence APYGADTPTA CCFSYGRQIP RKFIADYFET SSLCSQPGVI FLTKRNRQIC ADPKETWVQE YITELELNA

包装清单:

产品编号 产品名称 包装
P6906-5μg Recombinant Rat MIP-1α/CCL3 5μg
P6906-100μg Recombinant Rat MIP-1α/CCL3 100μg
P6906-500μg Recombinant Rat MIP-1α/CCL3 500μg
- 说明书 1份

保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。

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