| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 330496 | Q4VK74 | E. coli | 174aa | 19.7kDa | － |
| About this protein | |
| Name | Recombinant Murine IFN-λ2/IL-28A (Recombinant Murine Interferon-λ2/Interleukin-28A; rMuIFN-λ2/IL-28A)；Recombinant Mouse IFN-λ2/IL-28A ; 重组小鼠干扰素λ2/白细胞介素28A |
| Synonyms | IFN-lambda 2; interleukin-28A; IL28A; IL-28A; interferon, lambda 2 |
| Purity | >95% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by an anti-viral assay using human HepG2 cells infected with encephalomyocarditis is less than 3ng/ml, corresponding to a specific activity of >3.3×105IU/mg. |
| Physical Appearance | Sterile Filtered lyophilized (freeze-dried) liquid-like powder. |
| Formulation | Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 5% trehalose. |
| Endotoxin | Less than 0.1EU/μg of rMuIFN-λ2/IL-28A as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Interferon lambda-2, as known as IL-28A, belongs to the lambda interferon family. IL-28A (IFN-λ2), IL-28B (IFN-λ3) and IL-29 (IFN-λ1) are type III interferons that are class II cytokine receptor ligands. These IFNs signal through a receptor complex consisting of IL10R2 (also called CRF2-4) and IFNLR1 (also called CRF2-12). Interferon lambda-2 displays potent antiviral activity and antitumor activity, so it plays a significant role in the antiviral immune defense in the intestinal epithelium. It also upregulate MHC class I antigen expression. Recombinant murine interferon lambda-2 contains 174 amino acids and it shares 81% and 66%a.a. sequence identity with rat and human IFN-λ2, respectively, and functions across species. |
| Amino Acid Sequence | DPVPRATRLP VEAKDCHIAQ FKSLSPKELQ AFKKAKDAIE KRLLEKDMRC SSHLISRAWD LKQLQVQERP KALQAEVALT LKVWENMTDS ALATILGQPL HTLSHIHSQL QTCTQLQATA EPKPPSRRLS RWLHRLQEAQ SKETPGCLED SVTSNLFRLL TRDLKCVASG DQCV |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P6141-5μg | Recombinant Murine IFN-λ2/IL-28A | 5μg |
| P6141-20μg | Recombinant Murine IFN-λ2/IL-28A | 20μg |
| P6141-100μg | Recombinant Murine IFN-λ2/IL-28A | 100μg |
| P6141-1mg | Recombinant Murine IFN-λ2/IL-28A | 1mg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
