| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Mouse | 21937 | P25118 | E. coli | 191aa | 21.1kDa | － |
| About this protein | |
| Name | Recombinant Murine sTNF RI/TNFRSF1A (Recombinant Murine soluble Tumor Necrosis Factor Receptor Type I/TNFRSF1A; rMusTNF RI/TNFRSF1A)；Recombinant Mouse sTNF RI/TNFRSF1A ; 重组小鼠可溶性肿瘤坏死因子受体I型/TNFRSF1A |
| Synonyms | CD120a; TNFRSF1A; CD120a; CD120a antigen; FPF; p55; p55-R; p60; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNF-RI; TNF-R-I; TNFR-I; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit the TNF-α mediated cytotoxicity in the L-929 cells is less than 1μg/ml, corresponding to a specific activity of >1000IU/mg in the presence of 0.1ng/ml of rMuTNF-α. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS, pH7.4. |
| Endotoxin | Less than 0.1EU/μg of rMusTNF RI/TNFRSF1A as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | TNF RI, also named as TNFR-I, TNF R1, TNFRSF1A, p55 and p60, belongs to the tumor necrosis factor receptor superfamily and it is encoded by the TNFRSF1A gene in human. TNF RI is a universally expressed protein and is thought to form homotrimers after ligand binding. TNF RI is one of the major receptors for the TNF-α. This receptor can activate the transcription factor NF-κB, mediate apoptosis, and function as a regulator of inflammation. Murine TNF RI shares 70%, 88%, 67%, 70% and 64%a.a. sequence identity with human, rat, canine, feline and porcine TNF RI, respectively. |
| Amino Acid Sequence | IHPSGVTGLV PSLGDREKRD SLCPQGKYVH SKNNSICCTK CHKGTYLVSD CPSPGRDTVC RECEKGTFTA SQNYLRQCLS CKTCRKEMSQ VEISPCQADK DTVCGCKENQ FQRYLSETHF QCVDCSPCFN GTVTIPCKET QNTVCNCHAG FFLRESECVP CSHCKKNEEC MKLCLPPPLA NVTNPQDSGT A |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P6060-5μg | Recombinant Murine sTNF RI/TNFRSF1A | 5μg |
| P6060-20μg | Recombinant Murine sTNF RI/TNFRSF1A | 20μg |
| P6060-100μg | Recombinant Murine sTNF RI/TNFRSF1A | 100μg |
| P6060-1mg | Recombinant Murine sTNF RI/TNFRSF1A | 1mg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
