Recombinant Human IFN-α2c, Yeast
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产品编号 P5671-100μg
数量
¥ 3102.00
产品包装:
500μg 10μg 100μg 1mg
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Species Gene ID Accession Source Length MW Tag
Human 3440 P01563 Yeast 165aa 19.3kDa
About this Protein
Name Recombinant Human IFN-α2c, Yeast (Recombinant Human Interferon-alpha2c, Yeast; rHuIFN-α2c, Yeast);重组人干扰素-α2c,酵母
Synonyms IFNA2; IFNA2b; alpha-2a interferon; IFNA; IFN-alpha-2; IFN-alphaA; INFA2; interferon alpha 2b; interferon alpha A; interferon alpha-2; Interferon alpha-A; interferon, alpha 2; LeIF A; MGC125764; MGC125765
Purity >97% by SDS-PAGE and HPLC analyses.
Biological Activity Fully biologically active when compared to standard. The activity is determined by the cytopathic effect inhibition assay.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation Lyophilized from a 0.2µM filtered solution in PBS, pH7.4, with 0.02% Tween-20.
Endotoxin Less than 0.1EU/µg of rHuIFN-α2c, Yeast as determined by LAL method.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category Cytokine
Background IFN-αs are proteins secreted by leukocyte. They are mainly involved in innate immune response against viral infection. The IFN-α family has 13 subtypes and 23 different variants. The individual proteins have molecular masses between 19-26kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end.
Amino Acid Sequence CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRRDFGFPQ EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES LRSKE

包装清单:

产品编号 产品名称 包装
P5671-10µg Recombinant Human IFN-α2c, Yeast 10µg
P5671-100µg Recombinant Human IFN-α2c, Yeast 100µg
P5671-500µg Recombinant Human IFN-α2c, Yeast 500µg
- 说明书 1份

保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明,碧云天相关产品均为冻干粉,由于微量的蛋白在冻干过程中沉积在管内,形成很薄或不可见的蛋白层,所以在打开管盖前,我们建议在离心机中约8,000-12,000g离心10-30秒,使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的,一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子,即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡,以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献,或者根据实验目的,以及特定细胞和动物,通过实验进行摸索和优化。

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