| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Human | 3439 | P01562 | E. coli | 167aa | 19.5kDa | － |
| About this Protein | |
| Name | Recombinant Human IFN-α1a (Recombinant Human Interferon-α1a; rHuIFN-α1a)；重组人干扰素α1a |
| Synonyms | IFL; IFN; IFNA; IFNA13; IFN-ALPHA; IFN-alphaD |
| Purity | >97% by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The specific activity determined by an anti-viral assay is no less than 1.0×108IU/mg. |
| Physical Appearance | Sterile Filtered lyophilized (freeze-dried) liquid-like powder. |
| Formulation | Lyophilized from a 0.2μm filtered solution in PBS, pH7.4, containing 3% Mannitol, 5% Trehalose, 0.05% Tween-80. |
| Endotoxin | Less than 0.1EU/μg of rHuIFN-α1a as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions. |
| Category | Cytokine |
| Background | Interferon alpha-1, also named interferon alpha-D, is belonging to the type I interferon family and is encoded by the IFNA1gene in humans. Interferon alpha-1 is expressed in peripheral blood leukocytes and lymphoblastoid cells. IFNα-1 contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity. It plays an important role in inducing non-specific resistance against a broad range of viral infections, and also affects cell proliferation and modulates immune responses. IFNα-1a and IFNα-1b are two forms of interferon alpha-1 except for the substitution of alanine for valine at position 137. Interferon-alpha represents a group of related but distinct proteins that share over 95% amino acid sequence homology. |
| Amino Acid Sequence | MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNADSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE |
包装清单:
| 产品编号 | 产品名称 | 包装 |
| P5637-20μg | Recombinant Human IFN-α1a | 20μg |
| P5637-100μg | Recombinant Human IFN-α1a | 100μg |
| P5637-1mg | Recombinant Human IFN-α1a | 1mg |
| - | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品在冻干时添加了海藻糖作为保护剂，此时冻干后本产品看起来仍然像是液体，属于正常现象。在冻干工艺上，海藻糖作为常用的冻干保护剂，可显著阻止蛋白质二级结构的改变，防止冻干过程中的蛋白变性。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
使用说明：
1. 收到产品后请立即按照说明书推荐的条件保存。除非特别注明，碧云天相关产品均为冻干粉，由于微量的蛋白在冻干过程中沉积在管内，形成很薄或不可见的蛋白层，所以在打开管盖前，我们建议在离心机中约8,000-12,000g离心10-30秒，使附着在管盖或管壁上的蛋白聚集于管底。
2. 请根据实验目的并按照产品简介中Reconstitution一栏中的信息配制储存液。大多数细胞因子或重组蛋白的冻干粉是非常容易溶解的，一般用移液枪的枪头轻吹几下或者轻轻摇晃瓶子，即可使细胞因子或重组蛋白完全溶解。请勿用vortex剧烈振荡，以免蛋白变性而失活。
3. 具体的最佳工作浓度请自行参考相关文献，或者根据实验目的，以及特定细胞和动物，通过实验进行摸索和优化。
