Species | Gene ID | Accession | Source | Length | MW | Tag |
Streptomyces avidinii | X03591 | P22629 | E. coli | 128aa | 52kDa (tetramer) | - |
蛋白信息(About this protein) | |
名称(Name) | Recombinant Streptavidin |
别名(Synonyms) | Streptavidin;rStreptavidin;重组链霉亲和素;链霉亲和素;链霉抗生物素蛋白 |
产品简介(Background) | Beyotime's recombinant streptavidin was expressed in E. coli and purified. Streptavidin is a tetrameric protein produced by Streptomyces avidin. This protein binds the vitamin D-biotin with an affinity higher than any other known biological binding site (Kd~1×10-15M) [1]. The N and C terminal of the 159 residues full length protein is processed to give a shorter core streptavidin, usually composed of residues 13–139; removal of the N and C terminal is necessary for the highest biotin-binding affinity. The molecular mass for the native protein as ~52kDa per tetramer, but under extreme denaturing conditions, giving a major band at about ~13kDa. Streptavidin is a highly stable protein that can maintain its functional structure even under a variety of harsh conditions, such as high temperatures and the presence of high concentrations of denaturants and organic solvents. These unique properties, along with the ability of biotin to be incorporated easily into various biological materials without disturbing their properties and structures, allow streptavidin to serve as a powerful, versatile tool in biomedical science and clinical diagnostics [2]. |
产品用途(Applications) | Among the most common uses of streptavidin are the purification or detection of various biomolecules. The strong streptavidin-biotin interaction can be used to attach various biomolecules to one another or onto a solid support. This version of recombinant streptavidin has a higher binding capacity to Biotin than regular streptavidin when conjugated to magnetic beads or agarose beads. |
外观(Physical appearance) | Sterile colorless liquid |
活性(Biological activity) | >17U/mg (one unit binds 1µg D-biotin) |
浓度(Concentration) | 10mg/ml |
纯度(Purity) | >98% by SDS-PAGE |
储存液(Storage buffer) | Supplied as a 0.2μm filtered concentrated sterile solution in PBS. |
产品编号 | 产品名称 | 包装 |
P5085-1mg | Recombinant Streptavidin (重组链霉亲和素) | 1mg |
P5085-5mg | Recombinant Streptavidin (重组链霉亲和素) | 5mg |
P5085-20mg | Recombinant Streptavidin (重组链霉亲和素) | 20mg |
P5085-100mg | Recombinant Streptavidin (重组链霉亲和素) | 100mg |
— | 说明书 | 1份 |
-20ºC或更低温度保存,至少两年有效。由于蛋白的每次冻融均会引起部分失活,首次使用时建议分装后-20ºC或更低温度冻存,以避免反复冻融。
注意事项:本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
产品编号 | 产品名称 | 包装 |
A0303 | 辣根过氧化物酶标记Streptavidin | 0.2ml |
A0305 | 辣根过氧化物酶标记Streptavidin | 0.2/1/5ml |
A0312 | 碱性磷酸酯酶标记Streptavidin | 0.2ml |
P2151 | BeyoMag™ Streptavidin Magnetic Beads (链霉亲和素磁珠) | 200μl/1ml/5ml |
P5084 | Recombinant Streptavidin | 1/5/20/100mg |
P5085 | Recombinant Streptavidin (重组链霉亲和素) | 1/5/20/100mg |
P5087 | Monomeric Streptavidin (单体链霉亲和素) | 1/5/20/100mg |