| Species | Gene ID | Accession | Source | Length | MW | Tag |
| Aequorea victoria |
- | AYN77827 | E.coli | 271aa | 30.1kDa | C-Twin-Strep-Tag |
| 蛋白信息(About this protein) | |
| 名称(Name) | Recombinant mEGFP-Twin-Strep-tag，重组单体增强绿色荧光蛋白融合Twin-Strep-tag标签 |
| 别名(Synonyms) | monomeric Enhanced Green Fluorescent Protein with Twin-Strep-tag; mEGFP-Twin-Strep-tag Control Protein; mEGFP-Twin-Strep-tag II; EGFP重组蛋白 |
| 产品简介 (Background) | Beyotime's Recombinant mEGFP-Twin-Strep-tag was expressed in E.coli and purified, which contains the full length of mEGFP with Twin-Strep-tag at the C-terminus. mEGFP is one of the most widely used engineered variants of the original wild-type Green Fluorescent Protein (GFP). mEGFP exhibits greater fluorescence stability and brighter than the wild-type GFP. Twin-Strep-tag uses the principle of avidity to link two Strep-tag II together by linker (Twin-Strep-tag and linker sequence: WSHPQFEKGGGSGGGSGGSAWSHPQFEK). This peptide sequence exhibits intrinsic affinity towards Strep-Tactin or Strep-Tactin XT, a specifically engineered streptavidin, and can be N- or C-terminally fused to recombinant proteins. Twin-strep-tag improves the link between the target protein and Strep-Tactin or Strep-Tactin XT, improves the purification of low-concentration samples, and can also be used to grasp protein complexes and detect interactions between proteins [1-2]. |
| 产品用途 (Applications) | mEGFP-Twin-Strep-tag is suitable for test purifications, detections, and immobilizations with Strep-Tactin or Strep-Tactin XT; Protein transfection or microinjection in cells and tissues; Calibration of fluorescent protein detection instrumentation; WB standard. |
| 外观 (Physical appearance) | Liquid |
| 浓度 (Concentration) | 1mg/ml |
| 纯度(Purity) | >95% by SDS-PAGE |
| 储存液 (Storage buffer) | 10mM Tris pH8.0, 150mM NaCl, 1mM EDTA |
本产品在自然光下呈现绿色(图1左)，在紫外光(395-470nm)下呈现绿色荧光(图1右)。
图1. 碧云天Recombinant mEGFP-Twin-Strep-tag (P4403)在自然光与紫外光下效果对比图。本产品在自然光下呈现绿色(左)，在紫外光(395-470nm)下呈现绿色荧光(右)。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
本产品经SDS-PAGE电泳检测蛋白纯度和分子量参考图2。
图2. 碧云天Recombinant mEGFP-Twin-Strep-tag (P4403)的SDS-PAGE电泳检测图。本蛋白(图中简称mEGFP)经BeyoGel™ Elite PAGE预制胶(Bis-Tris, 12%, 10孔) (P0849)电泳，Marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD) (P0071/P0072)，并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
包装清单：| 产品编号 | 产品名称 | 包装 |
| P4403-50μg | Recombinant mEGFP-Twin-Strep-tag (1mg/ml) | 50μl |
| P4403-200μg | Recombinant mEGFP-Twin-Strep-tag (1mg/ml) | 200μl |
| P4403-1mg | Recombinant mEGFP-Twin-Strep-tag (1mg/ml) | 1ml |
| — | 说明书 | 1份 |
-20ºC保存，一年有效。-80ºC保存，可以保存更长时间。
注意事项：由于蛋白每次冻融均会引起部分失活，所以首次配制成相应浓度储存液后，须分装后-20ºC或更低温度冻存，尽量避免反复冻融。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| P4401 | Recombinant mEGFP-Strep-tag II | 50μg/200μg/1mg |
| P4403 | Recombinant mEGFP-Twin-Strep-tag | 50μg/200μg/1mg |
| P5082 | Recombinant Traptavidin (Streptavidin Mutant) | 0.5mg/2mg/10mg/50mg |
| P5083 | Recombinant Strep-Tactin Mutant | 1mg/5mg/20mg/100mg |
| AF2924 | StrepII Tag Mouse Monoclonal Antibody | 50μl |
| AF2925 | Recombinant Anti-Strep-tag II Antibody | 50μl |
| AF2927 | StrepII Tag Mouse Monoclonal Antibody (HRP Conjugated) | 50μl |
