Nitrilase,中文名为腈水解酶,催化腈水解生成相应的羧酸和氨。本产品为大肠杆菌表达的腈水解酶,并经简单纯化获得的可以确保达到一定酶活力的粗酶液。
由于本产品需新鲜制备,确认订购后约6个工作日才可以发货。个别情况首次制备出现问题,发货时间会顺延。
本产品可以提供十公斤、百公斤乃至吨级的粗酶液,如有需求请联系碧云天。
Nitrilase enzymes catalyse the hydrolysis of nitriles to carboxylic acids and ammonia, without the formation of "free" amide intermediates. Nitrilases are involved in natural product biosynthesis and post translational modifications in plants, animals, fungi and certain prokaryotes. Nitrilases can also be used as catalysts in preparative organic chemistry. Among others, nitrilases have been used for the resolution of racemic mixtures. Nitrilase should not be confused with nitrile hydratase (nitrile hydro-lyase; EC 4.2.1.84) which hydrolyses nitriles to amides. Nitrile hydratases are almost invariably co-expressed with an amidase, which converts the amide to the carboxylic acid, consequently it can sometimes be difficult to distinguish nitrilase activity from nitrile hydratase plus amidase activity. This product with the indicated enzyme activity was briefly purified from engineered E.coli.
产品详细信息见下表:
About this product | |
Name | Nitrilase (Crude Enzyme);腈水解酶(粗酶液) |
Application | 催化腈水解生成相应的羧酸和氨 |
Appearance | Clear to translucent yellow solution |
Enzyme activity | Undetermined |
About this enzyme | |
Name | Nitrilase;腈水解酶 |
CAS Number | 9024-90-2 |
EC Number | 3.5.5.1 |
Enzymatic Reaction | a nitrile + 2H2O = a carboxylate + NH3 |
Synonyms | acetonitrilase; benzonitrilase |
Systematic name | nitrile aminohydrolase |
Cofactor(s) | - |
Application | synthesis; industry; |
包装清单:
产品编号 | 产品名称 | 包装 |
P3753-100ml | Nitrilase (Crude Enzyme) | 100ml |
P3753-1L | Nitrilase (Crude Enzyme) | 1L |
— | 说明书 | 1份 |
保存条件:
-20℃或更低温度保存,至少1个月有效。
注意事项:
由于本产品需新鲜制备,在您确认订购后约6个工作日才能发货。个别情况首次制备出现问题,发货时间会顺延。
粗酶液的每次冻融均可能会引起部分失活,所以收到酶液后请根据需要进行分装,并置于-20℃或更低温度保存。
随着保存时间的延长,酶活力会有一定程度的下降,收到产品后应尽快使用。
本产品在生产和保存的过程中可能会有混浊或沉淀产生,融化后混匀即可,不影响正常使用。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用说明:
1.收到产品后请立即使用或按照说明书推荐的条件保存。随着保存时间的延长,酶活力会有一定程度的下降。
2.具体的最佳工作条件请自行参考相关文献,或者通过实验进行摸索和优化。
相关产品
产品编号 | 产品名称 | 包装 |
P3753-100ml | Nitrilase (Crude Enzyme) | 100ml |
P3753-1L | Nitrilase (Crude Enzyme) | 1L |