| Species | Gene ID | Accession | EC | Source | Length | MW | Tag |
| Human | 59272 | Q9BYF1 | 3.4.17.23 | HEK293 | 598aa (w/o tag) | ~120kDa | hFc |
| 蛋白信息(About this protein) | |
| 名称(Name) | Recombinant Human ACE2 Protein (hFc Tag)；Recombinant Human ACE2 Protein (hFc Tag) (重组人血管紧张素转换酶2蛋白，hFc Tag) |
| 别名(Synonyms) | Angiotensin I converting enzyme 2, ACE-Related Carboxypeptidase, Angiotensin-Converting Enzyme Homolog, ACEH, Metalloprotease MPROT15 |
| 产品简介(Background) | A DNA sequence encoding the mature extracellular domain (Gln18-Asp 615) of human ACE2 precursor (NP_068576.1) was expressed with the fused Fc region of human IgG4 (Glu98-Gly325) at the C-terminus. ACE2 belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. ACE2 is known to be expressed in various human organs, and its organ- and cell-specific expression suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronavirus HCoV-NL63 and the human severe acute respiratory syndrome coronaviruses, SARS-CoV and SARS-CoV-2, the latter is the causative agent of coronavirus disease-2019 (COVID-19). Multiple splice variants have been found for this gene and the dACE2 (or MIRb-ACE2) splice variant has been found to be interferon inducible. |
| 产品用途(Applications) | 用于血管紧张素转换酶2抑制剂的筛选、酶活性研究、抗体或结构研究 |
| 外观(Physical appearance) | 液体 |
| 活性(Biological activity) | ≥7,800U/mg (按定义1) 或7,800,000U/mg (按定义2) |
| 活力单位(Unit definition) | 定义1：温度37℃条件下，每分钟催化生成1nmol MCA的酶量定义为一个单位(Unit, U)。 定义2：温度37℃条件下，在一分钟内裂解1pmol荧光肽底物MCA-APK(Dnp)-OH所需的酶量定义为一个单位(Unit, U)。 |
| 浓度(Concentration) | 1mg/ml |
| 纯度(Purity) | ≥95% by SDS-PAGE |
| 配方(Formulation) | 0.1M phosphate buffer, 40% glycerol, pH8.0 |
| 使用方法(Recommended usage) | 用于抑制剂筛选时，一般每个反应体系(200μl)约需要1ng-1μg本蛋白。 |
| 氨基酸序列(Amino acid sequence) | QSTIEEQAKT FLDKFNHEAE DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAHLL GDMWGRFWTN LYSLTVPFGQ KPNIDVTDAM VDQAWDAQRI FKEAEKFFVS VGLPNMTQGF WENSMLTDPG NVQKAVCHPT AWDLGKGDFR ILMCTKVTMD DFLTAHHEMG HIQYDMAYAA QPFLLRNGAN EGFHEAVGEI MSLSAATPKH LKSIGLLSPD FQEDNETEIN FLLKQALTIV GTLPFTYMLE KWRWMVFKGE IPKDQWMKKW WEMKREIVGV VEPVPHDETY CDPASLFHVS NDYSFIRYYT RTLYQFQFQE ALCQAAKHEG PLHKCDISNS TEAGQKLFNM LRLGKSEPWT LALENVVGAK NMNVRPLLNY FEPLFTWLKD QNKNSFVGWS TDWSPYAD 备注：本氨基酸序列仅供参考，不含Fc序列，实际氨基酸长度或序列可能有一定变化。 |
| 产品编号 | 产品名称 | 包装 |
| P2325-50μg | Recombinant Human ACE2 Protein (hFc Tag) | 50μg |
| P2325-200μg | Recombinant Human ACE2 Protein (hFc Tag) | 200μg |
| P2325-1mg | Recombinant Human ACE2 Protein (hFc Tag) | 1mg |
| － | 说明书 | 1份 |
-20℃保存，一年有效。
注意事项：收到产品后请立即按照说明书推荐的条件保存。在打开管盖前，请适当离心，使附着在管盖或管壁上的蛋白聚集于管底。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
图1.Recombinant Human ACE2 Protein (hFc Tag)的电泳检测效果图。本蛋白经BeyoGel™ Plus PAGE预制胶(Hepes, 4-20%, 15孔) (P0524)电泳，marker为BeyoColor™彩色预染蛋白分子量标准(6.5-270kD)(P0071/P0072)，并经BeyoBlue™考马斯亮蓝超快染色液(P0017F)染色。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
图2. Recombinant Human ACE2 Protein (hFc Tag)酶切MCA-APK(Dnp)-OH底物的效果图。图A为0-60分钟内，不同量的ACE2酶切MCA-APK(Dnp)-OH生成MCA产物的荧光强度。图B为不同量的ACE2与MCA-APK(Dnp)-OH底物在37℃孵育1小时，生成MCA产物的荧光强度。实际检测结果可能会因样品和检测条件等的不同而存在差异，图中数据仅供参考。
