碱性磷酸酶检测试剂盒
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产品编号 P0321M
数量
¥ 1209.00
产品包装:
100次 500次
产品简介
使用说明
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碧云天生产的碱性磷酸酶检测试剂盒(Alkaline Phosphatase Assay Kit)是一种用于快速、便捷地检测细胞或组织样品的裂解或匀浆产物的上清液、血清、血浆、尿液等样品中内源性的碱性磷酸酶活性的试剂盒。
碱性磷酸酶(Alkaline Phosphatase, AP/ALP/AKP/ALKP/ALPase/Alk Phos)也称碱性磷酸酯酶(EC 3.1.3.1),可以在碱性条件下催化磷酸酯键的水解。哺乳动物中,肝脏、胆管、肾脏、骨头和胎盘中的碱性磷酸酶活性比较高。常见的碱性磷酸酶包括肠道碱性磷酸酶(alkaline phosphatase, intestinal, ALPI)、非组织特异性碱性磷酸酶(alkaline phosphatase, tissue-nonspecific isozyme, ALPL)和胎盘碱性磷酸酶(alkaline phosphatase, placental type, 也称placental alkaline phosphatase, PLAP)。常见的小牛肠碱性磷酸酶(Calf Intestinal Alkaline Phosphatase, CIAP/CIP)被广泛用于二抗等的标记最终用于蛋白和核酸等的检测,也常用于DNA或RNA 5'和3'末端的去磷酸化(去单磷酸化),特别是质粒的5'末端去磷酸化以避免质粒自连等。
干细胞,如iPS中,碱性磷酸酶的活性很高,常被用作iPS成功诱导的标志。另外,分化的结肠癌细胞中碱性磷酸酶的活性也会显著升高,被当作结肠癌细胞分化程度定性和定量的指标。此外,血清中碱性磷酸酯酶的升高,被称作高碱性磷酸酶血症(hyperalkalinephosphatasemia),被认为和恶性胆管阻塞(malignant biliary obstruction)、原发性胆管硬化(primary biliary cirrhosis)、原发性硬化胆管炎(primary sclerosing cholangitis)、肝淋巴瘤(hepatic lymphoma)和肝肉瘤(hepatic sarcoidosis)等肝胆疾病密切相关。血清中碱性磷酸酶活性升高还和骨头生成密切相关,因为碱性磷酸酶是成骨细胞的副产物。血清中碱性磷酸酶活性过低也和一些疾病相关。儿童和孕妇血清中的碱性磷酸酶活性较普通人高一些。血清中碱性磷酸酶活性范围在20-140U/L。
除胎盘碱性磷酸酶(alkaline phosphatase placental isoform)以外,其它的内源性碱性磷酸酶加热后易失活。
本试剂盒可以检测细胞或组织样品的裂解或匀浆产物的上清液、血浆、血清、尿液或纯化的酶样品等中的碱性磷酸酶活性。
Para-nitrophenyl phosphate (pNPP)是一种常用的磷酸酶显色底物,在碱性条件下,可在碱性磷酸酶作用下生成para-nitrophenol。para-nitrophenol(p-nitrophenol)在碱性条件下,呈黄色产物,可以在400-415nm检测吸光度。产物黄色越深,说明碱性磷酸酶检活性越高,反之则酶活性越低。据此通过比色分析就可以计算出碱性磷酸酶活性水平。
包括标准品和空白对照,本试剂盒P0321S包装共可进行100个样品的检测,P0321M包装共可进行500个样品的检测。
包装清单:

产品编号 产品名称 包装
P0321S-1 检测缓冲液 15ml
P0321S-2 显色底物 2管
P0321S-3 p-nitrophenol溶液(10mM) 0.1ml
P0321S-4 反应终止液 12ml
说明书 1份

产品编号 产品名称 包装
P0321M-1 检测缓冲液 65ml
P0321M-2 显色底物 5管
P0321M-3 p-nitrophenol溶液(10mM) 0.5ml
P0321M-4 反应终止液 60ml
说明书 1份

保存条件:
-20℃保存,一年有效。其中显色底物和p-nitrophenol溶液需避光保存。
注意事项:
如果希望进行酶活性的绝对定量,进行酶反应时必须注意精确计时。此时推荐采用孵育30分钟等较长的时间,以减小操作过程中的时间误差。同时如果样品中酶活性较高,则可以预先适当稀释样品。
样品溶液中须避免出现EDTA、氟离子、柠檬酸盐等碱性磷酸酶的抑制剂。
检测缓冲液和p-nitrophenol溶液对人体有害,操作时请小心,并注意有效防护以避免直接接触人体或吸入体内。反应终止液有腐蚀性,操作时请小心,并注意有效防护以避免直接接触人体或腐蚀其他物品。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 试剂准备:将所有试剂取出,恢复至室温使用。
a. 显色底物溶液:对于P0321S包装,请取一管显色底物,溶解于2.5ml的检测缓冲液中(可先用1ml检测缓冲液进行溶解,充分溶解和混匀后,转移至15ml离心管,再加入1.5ml检测缓冲液),充分溶解和混匀,冰上放置。对于P0321M包装,请取一管显色底物,溶解于5ml的检测缓冲液中(可先用2ml检测缓冲液进行溶解,充分溶解和混匀后,转移至15ml离心管,再加入3ml检测缓冲液),充分溶解和混匀,冰上放置。新鲜配制的显色底物溶液需在6小时内使用。
b. 标准品工作液:取10μl p-nitrophenol溶液(10mM),用检测缓冲液稀释至0.2ml,最终浓度为0.5mM。
2. 样品准备:
a. 细胞或组织裂解液的准备:采用适当细胞或组织裂解液裂解细胞或组织,建议使用碧云天的P0013J Western及IP细胞裂解液(无抑制剂)裂解相关样品。如果有必要需进行适当匀浆,随后离心取上清,用于碱性磷酸酶的检测。注意:裂解液中不能含有磷酸酶抑制剂。样品可以-80℃冻存,但需避免反复冻融。
b. 血浆、血清和尿液的准备:血浆和血清按照常规方法制备后可以直接用于本试剂盒的测定,但为了消除样品本身颜色的干扰,需设置加了血浆或血清但不加底物的对照。血浆制备时不能用含EDTA和柠檬酸盐的抗凝管。尿液通常也可以直接用于测定。上述样品可以-80℃冻存,但需避免反复冻融。
c. 样品的稀释:如果样品中含有较高活性的碱性磷酸酶,可以使用原有的裂解液或PBS等进行稀释,也可以采用试剂盒中的检测
缓冲液进行稀释。如果使用试剂盒中提供的检测缓冲液进行稀释,需注意保留足够的检测缓冲液用于试剂盒的检测过程。
3. 参考下表使用96孔板设置空白对照孔、标准品孔和样品孔。标准品的用量分别为4、8、16、24、32和40微升,样品通常可以直接加50微升。如果样品中的碱性磷酸酶活性过高,可以减少样品用量或适当稀释后再进行测定。

空白对照(Blank) 标准品(Standard) 样品(Sample)
检测缓冲液 50μl (100-x)μl (50-y)μl
显色底物 50μl 50μl
样品 yμl
标准品工作液 xμl

4. 用枪头轻轻吹打混匀,也可借助摇床进行混匀。
5. 37℃孵育5-10分钟。(说明:待测样品中碱性磷酸酶活性较低时,可适当延长孵育时间至30分钟)
6. 每孔加入100μl反应终止液终止反应。此时,标准品或有碱性磷酸酶活性的孔会呈现不同深浅的黄色。
7. 在405nm测定吸光度。如果不能测定405nm,也可以在400-415nm范围内检测吸光度。如果不能立即测定,可以在数小时内完成测定,所显现的黄色在数小时内稳定。
8. 碱性磷酸酶活性单位的定义:在pH9.8的diethanolamine(DEA)缓冲液中,37℃条件下,每分钟水解para-nitrophenyl phosphate显色底物产生1微摩尔p-nitrophenol所需的碱性磷酸酶的量定义为一个酶活力单位,也被称作一个DEA酶活力单位。在pH9.6的甘氨酸缓冲液中,25℃条件下,每分钟水解para-nitrophenyl phosphate显色底物产生1微摩尔p-nitrophenol所需的碱性磷酸酶的量定义为一个酶活力单位,也被称作一个Glycine酶活力单位。一个Glycine酶活力单位约相当于3个DEA酶活力单位。本试剂盒测定的是DEA酶活力单位。
9. 根据酶活性定义,计算出样品中的碱性磷酸酶活性。
相关产品:

产品编号 产品名称 包装
P0013J Western及IP细胞裂解液(无抑制剂) 100ml
P0321S 碱性磷酸酶检测试剂盒 100次
P0321M 碱性磷酸酶检测试剂盒 500次
P0326 酸性磷酸酶检测试剂盒 120次
P0329 胎盘碱性磷酸酶检测试剂盒 100次
P0332 抗酒石酸酸性磷酸酶检测试剂盒 120次
P0335 抗氟离子酸性磷酸酶检测试剂盒 120次


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使用本产品的文献:
1. Wang J, Gu Q, Hao J, Bai D, Liu L, Zhao X, Liu Z, Wang L, Zhou Q.
GENOM PROTEOM BIOINF . 2013 Oct;11(5):304-11. (IF 7.051)
2. Xie Q, Wang Z, Bi X, Zhou H, Wang Y, Gu P, Fan X.
BIOCHEM BIOPH RES CO . 2014 Mar 28;446(1):98-104. (IF 2.985)
3. Gao A, Hang R, Huang X, Zhao L, Zhang X, Wang L, Tang B, Ma S, Chu PK.
Biomaterials . 2014 Apr;35(13):4223-35. (IF 10.317)
4. Lyu Z, Wang H, Wang Y, Ding K, Liu H, Yuan L, Shi X, Wang M, Wang Y, Chen H.
Nanoscale . 2014 Jun 21;6(12):6959-69. (IF 6.895)
5. Jiang L, Peng WW, Li LF, Du R, Wu TT, Zhou ZJ, Zhao JJ, Yang Y, Qu DL, Zhu YQ.
J ENDODONT . 2014 Aug;40(8):1100-4. (IF 3.118)
6. Xue Y, Yan Y, Gong H, Fang B, Zhou Y, Ding Z, Yin P, Zhang G, Ye Y, Yang C, Ge J, Zou Y.
J Cell Biochem . 2014 Sep;115(9):1495-504. (IF 4.237)
7. Wu Y, Yang M, Fan J, Peng Y, Deng L, Ding Y, Yang R, Zhou J, Miao D, Fu Q.
Cell Death Dis . 2014 Oct 16;5:e1464. (IF 6.304)
8. Li LM, Ruan GX, HuangFu MY, Chen ZL, Liu HN, Li LX, Hu YL, Han M, Davidson G, Levkin PA, Gao JQ.
INT J PHARMACOL . 2015 Jul 5;488(1-2):1-11. (IF 0.692)
9. Liu Z, Tang Y, Kang T, Rao M, Li K, Wang Q, Quan C, Zhang C, Jiang Q, Shen H.
COLLOID SURFACE B . 2015 Jul 1;131:39-46. (IF 4.389)
10. Wu RX, Bi CS, Yu Y, Zhang LL, Chen FM.
Acta Biomater . 2015 Aug;22:70-82. (IF 7.242)
11. Tang XL, Wang CN, Zhu XY, Ni X.
Mol Cell Endocrinol . 2015 Sep 15;413:78-89. (IF 3.871)
12. Zhang Q,, Dong H,, Li Y,, Zhu Y, Zeng L,, Gao H,, Yuan B,, Chen X,, Mao C.
ACS APPL MATER INTER . 2015 Oct 21;7(41):23336-45. (IF 8.758)
13. Xie Q, Wang Z, Huang Y, Bi X, Zhou H, Lin M, Yu Z, Wang Y, Ni N, Sun J, Wu S, You Z, Guo C, Sun H, Wang Y, Gu P, Fan X.
Biomaterials . 2015 Oct;66:67-82. (IF 10.317)
14. Wang Z, Xie Q, Yu Z, Zhou H, Huang Y, Bi X, Wang Y, Shi W, Sun H, Gu P, Fan X.
SCI REP-UK . 2015 Oct 15;5:15280. (IF 3.998)
16. Tang Y, Xu Q, Peng H, Liu Z, Yang T, Yu Z, Cheng G, Li X, Zhang G, Shi R.
Atherosclerosis . 2015 Dec;243(2):357-63. (IF 3.919)
17. Huang C, Geng J, Wei X, Zhang R, Jiang S .
FEBS Lett . 2016 Mar;590(6):795-807. (IF 3.057)
18. Quan C, Tang Y, Liu Z, Rao M, Zhang W, Liang P, Wu N, Zhang C, Shen H, Jiang Q.
J BIOMED MATER RES B . 2016 Apr;104(3):576-84. (IF 2.831)
19. Kang H, Chen H, Huang P, Qi J, Qian N, Deng L,, Guo L.
OSTEOPOROSIS INT . 2016 Apr;27(4):1493-505. (IF 3.864)
22. Peng W, Shi Y, Li GF, He LG, Liang YS, Zhang Y, Zhou LB, Lin HR, Lu DQ .
FISH SHELLFISH IMMUN . 2016 Sep;56:388-96. (IF 3.298)
24. Zhou J, Zhu J, Jiang L, Zhang B, Zhu D, Wu Y .
Int J Cardiol . 2016 Oct 15;221:998-1003. (IF 3.229)
25. Qiu G, Wang P, Li G, Shi Z, Weir MD, Sun J, Song Y, Wang J, Xu HH, Zhao L .
Mol Biotechnol . 2016 Nov;58(11):748-756. (IF 2.022)
26. Tian Q, Wu S, Dai Z, Yang J, Zheng J, Zheng Q, Liu Y .
PeerJ . 2016 Nov 8;4:e2611. eCollection 2016. (IF 2.379)
27. Liu X, Liu R, Cao B, Ye K, Li S, Gu Y, Pan Z, Ding J .
Biomaterials. . 2016 Dec;111:27-39.
28. Tang XL, Wang CN, Zhu XY, Ni X .
Mol Cell Endocrinol . 2017 Jan 5;439:203-212. (IF 3.871)
29. Liu Y, Jiang D .
Cell Biol Int . 2017 Jan 19. doi: 10.1002/cbin.10730. [Epub ahead of print] (IF 2.571)
30. Liu X, Han F, Zhao P, Lin C, Wen X, Ye X .
Nanomedicine . 2017 Jan 25. pii: S1549-9634(17)30013-8. (IF 4.717)
31. Tang XL, Wang CN, Zhu XY, Ni X.
Mol Cell Endocrinol . 2017 Jan 5;439:203-212. (IF 3.871)
32. Chen H, Ji X, She F, Gao Y, Tang P .
Int J Mol Med . 2017 Feb;39(2):279-286. (IF 3.098)
33. Niu B, Li B, Gu Y, Shen X, Liu Y, Chen L .
J BIOMAT SCI-POLYM E . 2017 Feb;28(3):257-270. (IF 2.69)
34. Deng Z, Wang Z, Jin J, Wang Y, Bao N, Gao Q, Zhao J .
Acta Biomater . 2017 Feb;49:541-554. (IF 7.242)
35. Deng Z, Wang Z, Jin J, Wang Y, Bao N, Gao Q, Zhao J.
Acta Biomater . 2017 Feb;49:541-554. (IF 7.242)
36. Cheng L, Zhang L, Yang J, Hao L.
Exp Ther Med . 2017 Feb;13(2):467-474. (IF 1.785)
37. Chen H, Ji X, She F, Gao Y, Tang P.
Int J Mol Med . 2017 Feb;39(2):279-286. (IF 3.098)
38. Shen XQ, Geng YM, Liu P, Huang XY, Li SY, Liu CD, Zhou Z, Xu PP.
SCI REP-UK . 2017 Mar 20;7:44925. (IF 3.998)
39. Zhang Y, Dong C, Yang S, Wu J, Xiao K, Huang Y, Li X.
MAT SCI ENG C-MATER . 2017 Mar 1;72:464-471. (IF 5.88)
40. Guo S,Lu Y,Wu S,Liu L,He M,Zhao C,Gan Y,Lin J,Luo J,Xu X,Lin J
MAT SCI ENG C-MATER . 2017 Mar 1;72:631-640. (IF 5.88)
42. Chen X, Guo J, Yuan Y, Sun Z, Chen B, Tong X, Zhang L, Shen C, Zou J.
Mol Med Rep . 2017 May;15(5):2890-2896. (IF 2.1)
43. Chen Y, Gao A, Bai L, Wang Y, Wang X, Zhang X, Huang X, Hang R, Tang B, Chu PK.
MAT SCI ENG C-MATER . 2017 Jun 1;75:1049-1058. (IF 5.88)
44. Gao SY, Zheng GS, Wang L, Liang YJ, Zhang SE, Lao XM, Li K, Liao GQ.
PLoS One . 2017 Jun 8;12(6):e0179248. (IF 2.74)
45. Qian DY, Yan GB, Bai B, Chen Y, Zhang SJ, Yao YC, Xia H.
Biomed Pharmacother . 2017 Jun;90:492-499. (IF 4.545)
46. Chen B, Li L, Ren W, Yi L, Wang Y, Yan F.
Mol Med Rep . 2017 Jul;16(1):710-718. (IF 2.1)
47. Chen J, Lan Y, He Y, He C, Xu F, Zhang Y, Zhao Y, Liu Y.
Mol Med Rep . 2017 Aug;16(2):1801-1809. (IF 2.1)
48. Sun Y, Li Y, Wu B, Wang J, Lu X, Qu S, Weng J, Feng B.
Acta Biomater . 2017 Aug;58:527-538. (IF 7.242)
49. Wu Y, Wang M, Feng H, Peng Y, Sun J, Qu X, Li C.
Mol Cell Endocrinol . 2017 Sep 5;452:84-92. (IF 3.871)
50. Ou Q, Wang X, Wang Y, Wang Y, Lin X.
CELL PROLIFERAT . 2017 Oct 12. (IF 5.753)
51. Zheng Y, Li J, Wu J, Yu Y, Yao W, Zhou M, Tian J, Zhang J, Cui L, Zeng X, Liu Y.
Exp Ther Med . 2017 Oct;14(4):2845-2852. (IF 1.785)
52. Xiong Y, Zhang Y, Xin N, Yuan Y, Zhang Q, Gong P, Wu Y.
J STEROID BIOCHEM . 2017 Nov;174:153-160. (IF 3.813)
53. Lin FX,Zheng GZ,Chang B,Chen RC,Zhang QH,Xie P,Xie D,Yu GY,Hu QX,Liu DZ,Du SX,Li XD
CELL PHYSIOL BIOCHEM . 2018;47(1):161-175. (IF 5.5)
54. Dong M,Yu X,Chen W,Guo Z,Sui L,Xu Y,Shang Y,Niu W,Kong Y
CELL PHYSIOL BIOCHEM . 2018;49(3):884-898. (IF 5.5)
55. Xiao F,Wang C,Wang C,Gao Y,Zhang X,Chen X
CELL PHYSIOL BIOCHEM . 2018;45(5):1927-1939. (IF 5.5)
57. Qin X,Jiang T,Liu S,Tan J,Wu H,Zheng L,Zhao J
J Cell Biochem . 2018 Jan;119(1):1074-1082. (IF 4.237)
58. Deng A,Zhang H,Hu M,Liu S,Wang Y,Gao Q,Guo C
Exp Ther Med . 2018 Jan;15(1):789-794. (IF 1.785)
59. Gu X,Fu X,Lu J,Saijilafu,Li B,Luo ZP,Chen J
J Cell Biochem . 2018 Jan;119(1):1041-1049. (IF 4.237)
60. Zhang Y,Xiong Y,Chen X,Chen C,Zhu Z,Li L
Int Immunopharmacol . 2018 Jan;54:320-328. (IF 3.943)
61. Qin X,Jin P,Jiang T,Li M,Tan J,Wu H,Zheng L,Zhao J
MED SCI MONITOR. 2018 Jan 27;24:539-547. (IF 1.918)
62. Du X,Yu B,Pei P,Ding H,Yu B,Zhu Y
J Mater Chem B. 6(3):499-509. (IF 5.344)
63. Lin S,Cao L,Wang Q,Du J,Jiao D,Duan S,Wu J,Gan Q,Jiang X
J Mater Chem B. 6(3):414-427. (IF 5.344)
65. Cheng D,Liang Q,Li Y,Fan J,Wang G,Pan H,Ruan C
COLLOID SURFACE B . 2018 Feb 1;162:279-287. (IF 4.389)
66. Zhou X,Weng W,Chen B,Feng W,Wang W,Nie W,Chen L,Mo X,Su J,He C
J Mater Chem B. 6(5):740-752. (IF 5.344)
67. Luo J, Zhang H, Zhu J, Cui X, Gao J, Wang X, Xiong J.
COLLOID SURFACE B . 2018 Mar 1;163:369-378. (IF 4.389)
68. Wu Z,Dai W,Wang P,Zhang X,Tang Y,Liu L,Wang Q,Li M,Tang C
Connect Tissue Res . 2018 Mar;59(2):108-119. (IF 2.795)
70. Luo J,Zhang H,Zhu J,Cui X,Gao J,Wang X,Xiong J
COLLOID SURFACE B . 2018 Mar 1;163:369-378. (IF 4.389)
71. Zhang J,Feng Z,Wei J,Yu Y,Luo J,Zhou J,Li Y,Zheng X,Tang W,Liu L,Long J,Li X,Jing W
Int J Biol Sci . 2018 Mar 11;14(4):449-460. (IF 4.858)
72. Yan F, Wang C, Li T, Cai W, Sun J.
Mol Med Rep. 17(3):4237-4244. (IF 2.1)
73. Yu H,Pei T,Ren J,Ding Y,Wu A,Zhou Y
Mol Med Rep . 2018 Apr;17(4):6084-6092. (IF 2.1)
74. Zhao D,Liu Q,Han K,Wang H,Yang J,Bi K,Liu Y,Liu N,Tian Y,Li Y
Front Microbiol. (IF 4.235)
76. Li M,Yan J,Chen X,Tam W,Zhou L,Liu T,Pan G,Lin J,Yang H,Pei M,He F
J Cell Biochem . 2018 Jun;119(6):4928-4944. (IF 4.237)
77. Wang Q,Yang Q,Chen G,Du Z,Ren M,Wang A,Zhao H,Li Z,Zhang G,Song Y
SCI REP-UK . 2018 Jun 14;8(1):9127. (IF 3.998)
78. Hou Y,Cao X,Hu X,Li X,Shi X,Wang H,Peng C,Li J,Li J,Li Q,Wu C,Xiao X
Genes & Diseases . 2018 Jun 6;5(3):290-299.
79. Yan Z,Zhu S,Tian X,Ye Z,Zhai D,Zhu Z,Wei D,Zhu Q,Lu Z,Cao X
J Bone Miner Metab . 2018 Jul 21. (IF 2.297)
80. Zhao D,Liu Q,Huang X,Wang H,Han K,Yang J,Bi K,Liu Y,Zhang L,Li Y
J Vet Sci . 2018 Jul 31;19(4):528-535. (IF 1.561)
81. Shengyang Fu,Wei Liu,Shiwei Liu,Shichang Zhao,Yufang Zhu
Sci Technol Adv Mater. doi: 10.1080/14686996.2018.1471653.
82. Huang Z,Wu Z,Ma B,Yu L,He Y,Xu D,Wu Y,Wang H,Qiu G
ROY SOC OPEN SCI . 2018 Aug 1;5(8):172033. (IF 2.647)
83. Wang Y,Liu W,Liu Y,Cui J,Zhao Z,Cao H,Fu Z,Liu B
J Cell Physiol . 2018 Sep;233(9):7435-7446. (IF 5.546)
84. Hu H,Li Z,Lu M,Yun X,Li W,Liu C,Guo A
Biomed Pharmacother . 2018 Sep;105:66-72. (IF 4.545)
85. Zhang Y,Xiong Y,Zhou J,Xin N,Zhu Z,Wu Y
BIOCHEM BIOPH RES CO . 2018 Sep 10;503(3):1401-1408. (IF 2.985)
86. Wang W,Chen J,Hui Y,Huang M,Yuan P
BIOCHEM BIOPH RES CO . 2018 Sep 10;503(3):2186-2193. (IF 2.985)
88. Wu Y,Wang M,Zhang K,Li Y,Xu M,Tang S,Qu X,Li C
BIOCHEM BIOPH RES CO . 2018 Sep 5;503(2):737-743. (IF 2.985)
89. Hu XJ,Wu WC,Dong NG,Shi JW,Liu JW,Chen S,Deng C,Shi F
Curr Med Sci . 2018 Oct;38(5):765-775. (IF 1.273)
90. Zhao Y,Song G,Ren J,Li Q,Zhong S,Cui Z
Environ Mol Mutagen . 2018 Oct;59(8):687-697. (IF 3.131)
91. Wang F,Hu Y,He D,Zhou G,Ellis E 3rd
Am J Transl Res. 2018 Oct 15;10(10):2997-3010. eCollection 2018. (IF 3.375)
93. Han Y,Zhang K,Hong Y,Wang J,Liu Q,Zhang Z,Xia H,Tang Y,Li T,Li L,Xue Y,Hong W
FEBS Lett . 2018 Dec;592(24):4051-4065. (IF 3.057)
94. Jiang K,Huang D,Zhang D,Wang X,Cao H,Zhang Q,Yan C
Int J Biol Macromol . 2018 Dec;120(Pt A):170-179. (IF 5.162)
95. Zhang X,Chen J,Liu A,Xu X,Xue M,Xu J,Yang Y,Qiu H,Guo F
J Cell Physiol . 2018 Dec;233(12):9739-9749. (IF 5.546)
96. Zhang N,Ma L,Liu X,Jiang X,Yu Z,Zhao D,Zhang L,Zhang C,Huang F
Cell Tissue Bank . 2018 Dec;19(4):591-601. (IF 1.149)
97. Ye X,Li L,Lin Z,Yang W,Duan M,Chen L,Xia Y,Chen Z,Lu Y,Zhang Y
CARBOHYD POLYM . 2018 Dec 15;202:106-114. (IF 7.182)
98. Yan Y,Chen H,Zhang H,Guo C,Yang K,Chen K,Cheng R,Qian N,Sandler N,Zhang YS,Shen H,Qi J,Cui W,Deng L
Biomaterials. 190-191:97-110. (IF 10.317)
99. Li X,Song T,Chen X,Wang M,Yang X,Xiao Y,Zhang X
ACS APPL MATER INTER. 11(4):3722-3736. (IF 8.758)
101. Pan JM,Wu LG,Cai JW,Wu LT,Liang M
J RECEPT SIG TRANSD. 39(1):80-86. (IF 1.466)
102. Zhang T,Chen H,Zhang Y,Zan Y,Ni T,Liu M,Pei R
COLLOID SURFACE B. 174:528-535. (IF 4.389)
103. Wang R,Hu H,Guo J,Wang Q,Cao J,Wang H,Li G,Mao J,Zou X,Chen D,Tian W
J BIOMED NANOTECHNOL. 15(2):405-415. (IF 5.068)
105. Guo L,Wang X,Yuan J,Zhu M,Fu X,Xu RH,Wu C,Wu Y
SCI REP-UK. 9(1):2867. (IF 3.998)
106. Xu Z,Chen H,Wang Z,Fan F,Shi P,Tu M,Du M
J AGR FOOD CHEM. 67(5):1572-1584. (IF 4.192)
107. Yao S,Tan L,Chen H,Huang X,Zhao W,Wang Y
Stem Cells Int. 2019:3526409. (IF 3.869)
109. Tian T,Xie W,Gao W,Wang G,Zeng L,Miao G,Lei B,Lin Z,Chen X
Front Chem. 7:186. (IF 3.693)
110. Yuan X,Zhang M,Wang Y,Zhao H,Sun D
J BIOMAT SCI-POLYM E . 2019 Apr;30(5):355-375. (IF 2.69)
112. Wu J,Wang A,Wang X,Li G,Jia P,Shen G,Chen B,Yuan Y,Zhang H,Yang F,Xu Y
Bone. 121:16-28. (IF 4.147)
113. Gu H,Shi X,Liu C,Wang C,Sui N,Zhao Y,Gong J,Wang F,Zhang H,Li W,Zhao T
Nat Commun. 10(1):1465. (IF 12.121)
114. Zhang K,Wang M,Li Y,Li C,Tang S,Qu X,Feng N,Wu Y
AM J PHYSIOL-ENDOC M. 316(4):E590-E604. (IF 3.469)
116. Liu L,Wang D,Qin Y,Xu M,Zhou L,Xu W,Liu X,Ye L,Yue S,Zheng Q,Li D
FRONT ENDOCRINOL. 10:228. (IF 3.644)
117. Bing T,Shen L,Wang J,Wang L,Liu X,Zhang N,Xiao X,Shangguan D
Adv Sci (Weinh). 6(11):1900143.
118. Yuan FL,Xu RS,Ye JX,Zhao MD,Ren LJ,Li X
J Cell Mol Med. 23(5):3665-3675. (IF 4.486)
119. Zhao X,Zhou C,Lvov Y,Liu M
Small. 15(21):e1900357. (IF 11.459)
120. Zhou X,Li T,Chen Y,Zhang N,Wang P,Liang Y,Long M,Liu H,Mao J,Liu Q,Sun X,Chen H
Int J Oncol. 54(5):1843-1852. (IF 3.899)
121. Huang X,Wang F,Zhao C,Yang S,Cheng Q,Tang Y,Zhang F,Zhang Y,Luo W,Wang C,Zhou P,Kim S,Zuo G,Hu N,Li R,He TC,Zhang H
Stem Cells Dev. 28(10):683-694. (IF 3.082)
122. Huang Q,He C,Zhang J,Li W,Fu Y
Anal Chim Acta. 1055:98-105. (IF 5.977)
123. Ni C,Zhou J,Kong N,Bian T,Zhang Y,Huang X,Xiao Y,Yang W,Yan F
Biomaterials. 206:115-132. (IF 10.317)
125. Guo-Hui Zhao,Jian-Nan Liu,Xiao-Hua Hu,Khadija Batool,Liang Jin,Chen-Xu Wu,Juan Wu,Hong Chen,Xiao-Yan Jiang,Zhao-Hui Yang,Xian-Hui Huang,En-Jiong Huang,Xiao-Qiang Yu,Xiong Guan,Ling-Ling Zhang
PARASITE VECTOR. doi: 10.1186/s13071-019-3560-2. (IF 2.824)
126. Chen XW,Li YH,Zhang MJ,Chen Z,Ke DS,Xue Y,Hou JM
J Mol Endocrinol. 63(1):63-75. (IF 3.562)
127. Sheng JW,Liu DM,Jing L,Xia GX,Zhang WF,Jiang JR,Tang JB
Mol Med Rep. 20(1):198-204. (IF 2.1)
129. Ma Q,Liang M,Wu Y,Ding N,Duan L,Yu T,Bai Y,Kang F,Dong S,Xu J,Dou C
J Biol Chem. 294(29):11240-11247. (IF 4.238)
130. Jiao W,Zhang D,Wang D,Xu R,Tang L,Zhao M,Xu R
J Cell Mol Med. 23(8):5292-5302. (IF 4.486)
131. Liang X,Hou Z,Xie Y,Yan F,Li S,Zhu X,Cai L
J Cell Biochem. 120(8):13121-13132. (IF 4.237)
132. Zhang Y,Chen M,Tian J,Gu P,Cao H,Fan X,Zhang W
BIOMATER SCI-UK. 7(8):3266-3276. (IF 6.183)
134. Zhang M,Xie Y,Zhou Y,Chen X,Xin Z,An J,Hou J,Chen Z
BIOCHEM BIOPH RES CO. 516(1):300-306. (IF 2.985)
135. Xu X,Ding J,Wu X,Huang Z,Kong G,Liu Q,Yang Z,Huang Z,Zhu Q
EXP ANIM TOKYO. 68(3):371-380. (IF 1.574)
136. Huang XQ,Cen X,Sun WT,Xia K,Yu LY,Liu J,Zhao ZH
Am J Transl Res. 11(8):4776-4788. eCollection 2019. (IF 3.375)
137. Du J,Fan Y,Guo Z,Wang Y,Zheng X,Huang C,Liang B,Gao L,Cao Y,Chen Y,Zhang X,Li L,Xu L,Wu C,Weitz DA,Feng X
Cell Syst. 9(2):214-220.e5. (IF 8.673)
138. Li E,Shan H,Chen L,Long A,Zhang Y,Liu Y,Jia L,Wei F,Han J,Li T,Liu X,Deng H,Wang Y
Cell Metab. 30(2):319-328.e8. (IF 21.567)
139. Wang Y,Zhan J,Chen Y,Ai S,Li L,Wang L,Shi Y,Zheng J,Yang Z
Nanoscale. 11(29):13714-13719. (IF 6.895)
140. Wang W,Liu Y,Yang C,Qi X,Li S,Liu C,Li X
Int J Biol Sci. 15(10):2156-2169. (IF 4.858)
141. Yuanhao Zhang,Mingjiao Chen,Jia Tian,Ping Gu,Hongliang Cao,Xianqun Fan,Weian Zhang
BIOMATER SCI-UK. doi: 10.1039/c9bm00561g. (IF 6.183)
142. Zhang LT,Liu RM,Luo Y,Zhao YJ,Chen DX,Yu CY,Xiao JH
Life Sci. 232:116669. (IF 3.647)
143. Ma J,Shi C,Liu Z,Han B,Guo L,Zhu L,Ye T
AGING-US. 11(18):7537-7552. (IF 4.831)
145. Lu N,Lu Y,Liu S,Jin C,Fang S,Zhou X,Li Z
Biomacromolecules. 20(9):3485-3493. (IF 6.092)
148. Zheng F,Wang F,Xu Z
Exp Ther Med. 18(4):2660-2666. (IF 1.785)
150. Zhai Y,Wang Y,Rao N,Li J,Li X,Fang T,Zhao Y,Ge L
Front Physiol. 10:1304. (IF 3.367)
151. Zhu LF,Li L,Wang XQ,Pan L,Mei YM,Fu YW,Xu Y
Oral Dis. 25(8):1972-1982. (IF 2.613)
152. Zhang X,Lou Q,Wang L,Min S,Zhao M,Quan C
Biomed Mater. 15(1):015002. (IF 3.174)
153. Chang B,Qiu H,Zhao H,Yang X,Wang Y,Ji T,Zhang Y,Quan Q,Li Y,Zeng J,Meng H,Gu Y
MED SCI MONITOR. 25:8744-8752. (IF 1.918)
154. Luo ZW,Li FX,Liu YW,Rao SS,Yin H,Huang J,Chen CY,Hu Y,Zhang Y,Tan YJ,Yuan LQ,Chen TH,Liu HM,Cao J,Liu ZZ,Wang ZX,Xie H
Nanoscale. 11(43):20884-20892. (IF 6.895)
156. Chen S,Chu B,Chen Y,Cheng X,Guo D,Chen L,Wang J,Li Z,Hong Z,Hong D
J Cell Physiol. 234(12):22960-22971. (IF 5.546)
157. Hao M,He J,Wang C,Wang C,Ma B,Zhang S,Duan J,Liu F,Zhang Y,Han L,Liu H,Sang Y
Small. 15(51):e1905001. (IF 11.459)
158. Xiong Z,Xie Y,Yang Y,Xue Y,Wang D,Lin S,Chen D,Lu D,He L,Song B,Yang Y,Sun X
J Cell Mol Med. 23(12):8046-8057. (IF 4.486)
159. Shao N,Guan Y,Liu S,Li X,Zhou D,Huang Y
Macromol Biosci. 19(12):e1900255. (IF 3.416)
160. Huang X,Cen X,Zhang B,Liao Y,Zhao Z,Zhu G,Zhao Z,Liu J
J Cell Mol Med. 23(12):8432-8441. (IF 4.486)
161. Aimaiti A,Wahafu T,Keremu A,Yicheng L,Li C
Biol Trace Elem Res. (IF 2.639)
162. Yu HF,Duan CC,Yang ZQ,Wang YS,Yue ZP,Guo B
Oxid Med Cell Longev. 2019:6170936. (IF 5.076)
163. Sun M,Hu L,Wang S,Huang T,Zhang M,Yang M,Zhen W,Yang D,Lu W,Guan M,Peng S
J Bone Miner Res. 35(2):306-316. (IF 5.854)
164. Xiangyu Zhang,Guannan Zhang,Maozhou Chai,Xiaohong Yao,Weiyi Chen,Paul K Chu
Bioact Mater. doi: 10.1016/j.bioactmat.2020.07.017.
165. Xiaoyu Han,Mingjie Sun,Bo Chen,Qimanguli Saiding,Junyue Zhang,Hongliang Song,Lianfu Deng,Peng Wang,Weiming Gong,Wenguo Cui
Bioact Mater. doi: 10.1016/j.bioactmat.2020.11.019.
166. Xiaohan Dai,Boon Chin Heng,Yunyang Bai,Fuping You,Xiaowen Sun,Yiping Li,Zhangui Tang,Mingming Xu,Xuehui Zhang,Xuliang Deng
Bioact Mater. doi: 10.1016/j.bioactmat.2020.12.020.
167. Lan Ma,Yijun Yu,Hanxiao Liu,Weibin Sun,Zitong Lin,Chao Liu,Leiying Miao
Sci Rep. doi: 10.1038/s41598-020-79734-9.
168. Weiping Shi,Danhua Ling,Feiyun Zhang,Xiaohui Fu,Danping Lai,Yanzhen Zhang
Arch Oral Biol. doi: 10.1016/j.archoralbio.2020.104958.
169. Jia-Yan Chen,You-Xiang Wang,Ke-Feng Ren,Yun-Bing Wang,Guo-Sheng Fu,Jian Ji
Colloids Surf B Biointerfaces. doi: 10.1016/j.colsurfb.2020.111388.
170. Weiyuan Ye,Ya Wang,Sasa Hou,Bing Mei,Xinhong Liu,Han Huang,Qian Zhou,Yajing Niu,Yuanyuan Chen,Manling Zhang,Qingyang Huang
Hum Mutat. doi: 10.1002/humu.24126.
171. Shan He,Hanxiang Zhang,Yang Lu,Zhaosi Zhang,Xiang Zhang,Nian Zhou,Zhenming Hu
Aging (Albany NY). doi: 10.18632/aging.202434.
172. Haifeng Hu,Dong Wang,Lihong Li,Haiyang Yin,Guoyu He,Yonghong Zhang
Cell Death Dis. doi: 10.1038/s41419-021-03430-3.
173. Guannan Zhang,Yongqiang Yang,Jing Shi,Xiaohong Yao,Weiyi Chen,Xiaochun Wei,Xiangyu Zhang,Paul K Chu
Biomaterials. doi: 10.1016/j.biomaterials.2020.120634.
174. Wangxing Hu,Rongrong Wu,Chenyang Gao,Feng Liu,Zhiru Zeng,Qifeng Zhu,Jinyong Chen,Si Cheng,Kaixiang Yu,Yi Qian,Jing Zhao,Shuhan Zhong,Qingju Li,Lihan Wang,Xianbao Liu,Jian'an Wang
FASEB J. doi: 10.1096/fj.202001588RR.
176. Lulu Zhao,Shengnan Wang,Hong Liu,Xiaoli Du,Ren Bu,Bing Li,Ruilan Han,Jie Gao,Yang Liu,Jian Hao,Jianrong Zhao,Yan Meng,Gang Li
Front Cell Dev Biol. doi: 10.3389/fcell.2021.639127.
177. Guanning Wei,Hongmei Sun,Haijun Wei,Tao Qin,Yifeng Yang,Xiaohong Xu,Shoujing Zhao
Int J Mol Sci. doi: 10.3390/ijms22094715.
178. Jianxu Wei,Xiaomeng Zhang,Yuan Li,Xinxin Ding,Yi Zhang,Xue Jiang,Hongchang Lai,Junyu Shi
Acta Biochim Biophys Sin (Shanghai). doi: 10.1093/abbs/gmab037.
179. Qun Huang,Hao Chai,Shendong Wang,Yongming Sun,Wei Xu
Mol Med Rep. doi: 10.3892/mmr.2021.12018.
181. Linshan Xu,Yuyang Wang,Jianping Wang,Jianglong Zhai,Li Ren,Guoying Zhu
Int J Mol Sci. doi: 10.3390/ijms22179323.
182. Lili Fan,Yanmei Zhang,Jiejie Hu,Yuan Fang,Ren Hu,Wei Shi,Bin Ren,Changjian Lin,Zhong-Qun Tian
ACS Omega. doi: 10.1021/acsomega.1c03278.
183. Fengyu Huang,Zhiping Zeng,Weidong Zhang,Zhiqiang Yan,Jiayun Chen,Liangfa Yu,Qian Yang,Yihuan Li,Hongyu Yu,Junjie Chen,Caisheng Wu,Xiao-Kun Zhang,Ying Su,Hu Zhou
Eur J Med Chem. doi: 10.1016/j.ejmech.2021.113542.
184. Jiaqi Xing,Xu Peng,Anqi Li,Meilin Chen,Yuan Ding,Xinyuan Xu,Peng Yu,Jing Xie,Jianshu Li
Carbohydr Polym. doi: 10.1016/j.carbpol.2021.118382.
185. Xiaoya Gao,Yun Xue,Kechun Yang
Exp Ther Med. doi: 10.3892/etm.2021.10505.
186. Zihao Zhang,Xinyu Zhang,Chengwei Wang,Peng Zhou,Jie Xiao,Hui Zheng,Lei Wang,Senbo Yan,Yue Zhang,Xiaoping Ji
Exp Ther Med. doi: 10.3892/etm.2021.10586.
187. Zhanrong Kang,Dejian Li,Chaoqin Shu,Jianhang Du,Bin Yu,Zhi Qian,Zeyuan Zhong,Xu Zhang,Baoqing Yu,Qikai Huang,Jianming Huang,Yufang Zhu,Chengqing Yi,Huifeng Ding
Front Bioeng Biotechnol. doi: 10.3389/fbioe.2021.749221.
188. Majed G Alrowaili,Abdelaziz M Hussein,Elsayed A Eid,Mohamed S Serria,Hussein Abdellatif,Hussein F Sakr
J Bone Metab. doi: 10.11005/jbm.2021.28.4.307.