RIPA裂解液(强中弱套装)
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产品编号 P0013E
数量
¥ 281.00
产品包装:
共150ml
产品简介
使用说明
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碧云天生产的RIPA裂解液(RIPA Lysis Buffer)是一种传统的细胞组织快速裂解液。RIPA裂解液裂解得到的蛋白样品可以用于常规的Western、IP和ELISA等。
RIPA的本意是Radio Immunoprecipitation Assay。RIPA裂解液的配方有很多种,根据其裂解液的强度大致可以分为强、中、弱三类。关于不同的RIPA裂解液以及碧云天生产的其它裂解液的主要特点和差异,以及如何选择裂解液可参考我们的相关网页:http://www.beyotime.com/support/lysis-buffer.htm
RIPA裂解液(强中弱套装)含有RIPA裂解液(强)、RIPA裂解液(中)和RIPA裂解液(弱)各50ml,便于实验时探索不同的实验条件。
用RIPA裂解液裂解得到的蛋白样品,可以用碧云天生产的BCA蛋白浓度测定试剂盒(P0009/P0010/P0010S/P0011/P0012/
P0012S)测定蛋白浓度。由于含有较高浓度的去垢剂,不能用Bradford法测定由本裂解液裂解得到样品的蛋白浓度.
包装清单:

产品编号 产品名称 包装
P0013E-1 RIPA裂解液(强) 50ml
P0013E-2 RIPA裂解液(中) 50ml
P0013E-3 RIPA裂解液(弱) 50ml
说明书 1份

保存条件:
-20℃保存,一年有效。
注意事项:
为取得最佳的使用效果,尽量避免过多的反复冻融。可以适当分装后使用。
需自备PMSF。PMSF(ST506)可以向碧云天订购。
裂解样品的所有步骤都需在冰上或4℃进行。
关于裂解液的选择,一方面可以参考我们的相关网页:http://www.beyotime.com/support/lysis-buffer.htm选择合适的裂解液;另一方面也需要通过一些预实验来摸索最佳的适合您实验条件的裂解液。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
对于培养细胞样品:
1.融解RIPA裂解液,混匀。取适当量的裂解液,在使用前数分钟内加入PMSF,使PMSF的最终浓度为1mM。
2.对于贴壁细胞:去除培养液,用PBS、生理盐水或无血清培养液洗一遍(如果血清中的蛋白没有干扰,可以不洗)。按照6孔板每孔加入150-250微升裂解液的比例加入裂解液。用枪吹打数下,使裂解液和细胞充分接触。通常裂解液接触细胞1-2秒后,细胞就会被裂解。
对于悬浮细胞:离心收集细胞,用手指把细胞用力弹散。按照6孔板每孔细胞加入150-250微升裂解液的比例加入裂解液。再用手指轻弹以充分裂解细胞。充分裂解后应没有明显的细胞沉淀。如果细胞量较多,必需分装成50-100万细胞/管,然后再裂解。
3.充分裂解后,10000-14000g离心3-5分钟,取上清,即可进行后续的PAGE、Western和免疫沉淀等操作。
裂解液用量说明:通常6孔板每孔细胞加入150微升裂解液已经足够,但如果细胞密度非常高可以适当加大裂解液的用量到200微升或250微升。每100万细胞用100微升RIPA裂解后获得的上清,其蛋白浓度约为2-4mg/ml,不同细胞有所不同。
对于组织样品:
1.把组织剪切成细小的碎片。
2.融解RIPA裂解液,混匀。取适当量的裂解液,在使用前数分钟内加入PMSF,使PMSF的最终浓度为1mM。
3.按照每20毫克组织加入150-250微升裂解液的比例加入裂解液。(如果裂解不充分可以适当添加更多的裂解液,如果需要高浓度的蛋白样品,可以适当减少裂解液的用量。)
4.用玻璃匀浆器匀浆,直至充分裂解。
5.充分裂解后,10000-14000g离心3-5分钟,取上清,即可进行后续的PAGE、Western和免疫沉淀等操作。每20mg冻存的小鼠肝脏组织用200微升RIPA裂解液裂解后获得的上清,其蛋白浓度约为15-25mg/ml,不同状态的不同组织有所不同。
6.如果组织样品本身非常细小,可以适当剪切后直接加入裂解液裂解,通过强烈vortex使样品裂解充分。然后同样离心取上清,用于后续实验。直接裂解的优点是比较方便,不必使用匀浆器,缺点是不如使用匀浆器那样裂解得比较充分。
注:RIPA裂解液的裂解产物中经常会出现一小团透明胶状物,属正常现象。该透明胶状物为含有基因组DNA等的复合物。在不检测和基因组DNA结合特别紧密的蛋白的情况下,可以直接离心取上清用于后续实验;如果需要检测和基因组结合特别紧密的蛋白,则可以通过超声处理打碎打散该透明胶状物,随后离心取上清用于后续实验。如果检测一些常见的转录因子,例如NF-kappaB、p53等时,通常不必进行超声处理,就可以检测到这些转录因子。

附录:碧云天生产的各种裂解液主要特点、差异和选择
首先请参考下表,了解各种裂解液的主要特点和差异。

产品编号 P0013 P0013B P0013C P0013D P0013F P0013G P0013J P0013K
产品名称 Western及IP细胞裂解液 RIPA裂解液(强) RIPA裂解液(中) RIPA裂解液(弱) NP-40裂解液 SDS裂解液 Western及IP细胞裂解液(无抑制剂) RIPA裂解液(强,无抑制剂)
有效裂解成分 1% Triton X-100 1% Triton X-100, 1% deoxycholate, 0.1% SDS 1% NP-40, 0.5% deoxycholate, 0.1% SDS 1% NP-40, 0.25% deoxycholate 1% NP-40 1% SDS 1% Triton X-100 1% Triton X-100, 1% deoxycholate, 0.1% SDS
裂解强度 温和 温和 温和 温和
对膜蛋白的提取 一般 很好 较好 一般 一般 很好 一般 很好
对胞浆蛋白的提取 很好 很好 很好 很好 很好 很好 很好 很好
对核蛋白的提取 较好 很好 较好 较好 较好 很好 较好 很好
胞浆磷酸化蛋白提取 很好 很好 很好 很好 很好 很好 很好 很好
细胞核转录因子提取 很好 很好 很好 很好 很好 很好 很好 很好
含蛋白酶抑制剂
含磷酸酯酶抑制剂
主要用途 WB, IP,co-IP WB, IP WB, IP WB, IP, co-IP WB, IP,co-IP WB, ChIP WB, IP,co-IP WB, IP

用于普通的Western、IP或co-IP,我们推荐使用Western及IP细胞裂解液(P0013),该裂解液已被国内各大研究机构广泛使用,发表大量SCI论文,用户普遍反映很好。裂解细胞或组织后,没有非常粘滞的透明状DNA团块形成,不必采用超声处理等就可以非常理想地用于后续操作。另外该裂解液裂解的产物也适合用于磷酸化蛋白的Western检测。
对于某些特殊蛋白的IP,如果发现Western及IP细胞裂解液(P0013)效果不是非常理想,可以尝试用RIPA裂解液(强、中或弱)或NP-40裂解液。如果发现IP的时候背景很高,即非特异的蛋白也被IP下来,则需要选用裂解强度较高的裂解液,例如RIPA裂解液(强或中)。如果发现目的蛋白无法被IP下来,则说明裂解液的强度过强,可以使用较为温和的裂解液例如RIPA裂解液(弱)或NP-40裂解液。
对于某些难溶解蛋白的Western,如果发现Western及IP细胞裂解液(P0013)效果不是非常理想,可以尝试使用裂解强度更高的裂解液例如RIPA裂解液(强、中)或SDS裂解液。使用RIPA裂解液(强)的用户也非常多,发表了大量SCI论文。
用于特定用途需要自行添加特定抑制剂或不需要添加抑制剂时,可以考虑选购P0013JP0013KP0013J在很多时候可以兼容酶活性和生物小分子的检测,对于特定的酶或生物小分子的检测是否兼容需 要自行测试,碧云天不提供具体的应用信息。P0013J的裂解能力比P0013K弱一些,但用于酶活性和生物小分子时,P0013J的兼容性通常会更好一些。

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82. He Y, Shang X, Sun J, Zhang L, Zhao W, Tian Y, Cheng H, Zhou R.
J Exp Zool B Mol Dev Evol 2010 Jun 15;314(4):257-66. (IF 1.14)
83. Sun H, Li QW, Lv XY, Ai JZ, Yang QT, Duan JJ, Bian GH, Xiao Y, Wang YD, Zhang Z, Liu YH, Tan RZ, Yang Y, Wei YQ, Zhou Q.
Mol Biol Rep 2010 Jul;37(6):2951-8. (IF 1.402)
84. Hu L, Zhang J, Zhu H, Min J, Feng Y, Zhang H.
Med Oncol 2010 Sep;27(3):708-14. (IF 2.834)
85. J. Dang, R.Jia, Y.Tu, S.Xiao, G.Ding.
BIOMED PHARMACOTHER 2010 Dec;64(10):681-5. (IF 4.545)
87. Liu H, Sun H, Liu C.
CHINESE J PHYSIOL 2011 Apr 30;54(2):124-32. (IF 1.151)
88. Cai L, Bian M, Liu M, Sheng Z, Suo H, Wang Z, Huang F, Fei J.
Neuroscience 2011 May 5;181:196-205. (IF 3.056)
89. Lin F, Wang X, Jie Z, Hong X, Li X, Wang M, Yu Y.
J HUAZHONG U SCI-MED 2011 Aug;31(4):509-14. (IF 1.151)
90. Xiao HB, Cao X, Wang L, Run XQ, Su Y, Tian C, Sun SG, Liang ZH.
CHINESE MED J-PEKING 2011 Sep;124(17):2628-35. (IF 1.585)
91. Liu N, Huang H, Lin F, Chen A, Zhang Y, Chen R, Du H.
Neuroscience 2011 Oct 27;194:349-58. (IF 3.056)
92. Wang SB, Shi Q, Xu Y, Xie WL, Zhang J, Tian C, Guo Y, Wang K, Zhang BY, Chen C, Gao C, Dong XP.
PLoS One 2012;7(6):e38221. (IF 2.74)
93. Wang D, Tang F, Wang S, Jiang Z, Zhang L.
CANCER CHEMOTH PHARM 2012 Jan;69(1):173-83. (IF 2.967)
94. Yang XL, Guo TK, Wang YH, Huang YH, Liu X, Wang XX, Li W, Zhao X, Wang LP, Yan S, Wu D, Wu YJ.
Int Immunopharmacol 2012 Feb;12(2):408-14. (IF 3.943)
95. Liu Y, Xiao Z, Yang D, Ren L, Liu G, Yang L.
Molecules 2012 Feb 1;17(2):1307-18. (IF 3.267)
96. Wang C, Zhang L, He Q, Feng X, Zhu J, Xu Z, Wang X, Chen F, Li X, Dong J.
Mol Med Rep 2012 Feb;5(2):410-4. (IF 2.1)
98. Wang J, Sun LL, Zhu WL, Sun Y, Liu JF, Lu L, Shi J.
PSYCHOPHARMACOLOGY 2012 Mar;220(1):117-28. (IF 3.13)
99. Lu B, Xu Y, Xu L, Cong X, Yin L, Li H, Peng J.
ENVIRON TOXICOL CHEM 2012 Mar 30;34(2):127-135. (IF 3.152)
100. Deng J, Huang Q, Wang F, Liu Y, Wang Z, Wang Z, Zhang Q, Lei B, Cheng Y.
J Mol Neurosci 2012 Mar;46(3):677-87. (IF 2.678)
101. Yu L, Liu Y, Qiu Z, Liu S, Gao X, Zhu D.
J RECEPT SIG TRANSD 2012 Apr;32(2):87-95. (IF 1.466)
102. Li J, Guo Y, Liang X, Sun M, Wang G, De W, Wu W.
J CANCER RES CLIN 2012 May;138(5):763-74. (IF 3.656)
103. Ding D, Xu H, Liang Q, Xu L, Zhao Y, Wang Y.
Int Orthop 2012 May;36(5):1087-94. (IF 2.854)
104. Ma Y, Niu R, Sun Z, Wang J, Luo G, Zhang J, Wang J.
Arch Toxicol 2012 Jun;86(6):849-56. (IF 5.059)
106. Wang Y, Liu Q, Liu Z, Li B, Sun Z, Zhou H, Zhang X, Gong Y, Shao C.
MUTAT RES-REV MUTAT 2012 Jun 1;734(1-2):20-9. (IF 5.803)
107. Mao SS, Hua R, Zhao XP, Qin X, Sun ZQ, Zhang Y, Wu YQ, Jia MX, Cao JL, Zhang YM.
J NEUROTRAUM 2012 Jul 1;29(10):1941-59. (IF 3.793)
108. Hao M, Kuang HY, Fu Z, Gao XY, Liu Y, Deng W.
Neurochem Int 2012 Jul;61(1):1-6. (IF 3.881)
109. Yang XH, Wang YH, Wang JJ, Liu YC, Deng W, Qin C, Gao JL, Zhang LY.
Peptides 2012 Jul;36(1):60-70. (IF 2.843)
111. Jia LQ, Yang GL, Ren L, Chen WN, Feng JY, Cao Y, Zhang L, Li XT, Lei P.
J Ethnopharmacol 2012 Aug 30;143(1):100-8. (IF 3.69)
112. Liu K, Wang H, Long Y, Ye J, Yuan L.
J Cell Biochem 2012 Sep;113(9):2909-19. (IF 4.237)
113. Zhang Y, Zhou L, Zhang X, Bai J, Shi M, Zhao G.
Neurol Sci 2012 Oct;33(5):1125-31. (IF 2.415)
114. Ding D, Wang Z, Xu H, Xu L, Liang Q, Zhao Y, Shi Q, Wang Y.
南方医科大学学报 2012 Oct;32(10):1383-8.
115. Gao Y, Zhang RR, Li JH, Ren M, Ren ZX, Shi JH, Pan QZ, Ren SP.
Endocrine 2012 Dec;42(3):592-8. (IF 3.235)
117. Shou Y, Yang Y, Xu MS, Zhao YQ, Ge LB, Zhang BM.
EVID-BASED COMPL ALT 2013;2013:393460. (IF 1.813)
118. Gao L, Song JR, Zhang JW, Zhao X, Zhao QD, Sun K, Deng WJ, Li R, Lv G, Cheng HY, Wei LX.
Int J Biol Sci 2013;9(4):322-30. (IF 4.858)
120. Liu L, Liu L, Bo T, Li S, Zhu Z, Cui R, Mao D.
International Journal of Endocrinology 2013;2013:786574. (IF 2.287)
121. Shen R, Xie F, Shen H, liu Q, Zheng T, Kou X, Wang D, Yang J.
PLoS One 2013;8(2):e56542. (IF 2.74)
123. Ye HH, Wu KJ, Fei SJ, Zhang XW, Liu HX, Zhang JL, Zhang YM.
CLIN RES HEPATOL GAS 2013 Feb;37(1):e3-15. (IF 2.718)
124. Yuan L, Zhang L, Dong X, Zhao H, Li S, Han D, Liu X.
TUMOR BIOL  2013 Feb;34(1):577-85. (IF 3.65)
125. Li A, Lu D, Zhang Y, Li J, Fang Y, Li F, Sun J.
Oncol Rep 2013 Feb;29(2):535-40. (IF 3.417)
126. Chen X, Chen L, Zhang R, Yi Y, Ma Y, Yan K, Jiang X, Wang X.
Neurosci Lett 2013 Mar 14;537:44-9. (IF 2.274)
127. Wei Q, Ren X, Jiang Y, Jin H, Liu N, Li J.
BMC CARDIOVASC DISOR 2013 Mar 5;13:13. (IF 2.078)
128. Liu W, Otkur W, Li L, Wang Q, He H, Zang L, Hayashi T, Tashiro S, Onodera S, Xia M, Ikejima T.
BIOCHEM BIOPH RES CO 2013 Mar 8;432(2):314-9. (IF 2.985)
129. Xu D, Zhang T, Chen X, Zhou Q, Liu C, Deng Z, Zhang L, Ying C, Zhang W, Gu M.
TRANSPL P 2013 Mar;45(2):659-65. (IF 0.784)
130. Zhu X, Jiang Y, Shan PF, Shen J, Liang QH, Cui RR, Liu Y, Liu GY, Wu SS, Lu Q, Xie H, Liu YS, Yuan LQ, Liao EY.
Amino Acids 2013 Mar;44(3):961-8. (IF 3.063)
131. Xu J, Jia YY, Chen SR, Ye JT, Bu XZ, Hu Y, Ma YZ, Guo JL, Liu PQ.
Int Immunopharmacol 2013 Apr;15(4):743-51. (IF 3.943)
132. Mo QQ, Chen PB, Jin X, Chen Q, Tang L, Wang BB, Li KZ, Wu P, Fang Y, Wang SX, Zhou JF, Ma D, Chen G.
ACTA PHARMACOL SIN 2013 Apr;34(4):541-8. (IF 5.064)
133. Sun LF, Shi C, Yuan L, Sun Y, Yao XX, Ma JW, Huang CM, Zhu HF, Lei P, Shen GX.
J HUAZHONG U SCI-MED 2013 Apr;33(2):172-7. (IF 1.151)
135. Yang J, Huang H, Yang H, He X, Jiang X, Shi Y, Alatangaole D, Shi L, Zhou N.
J Biol Chem 2013 Apr 26;288(17):11662-75. (IF 4.238)
136. Li G, Zhang S, Fang H, Yan B, Zhao Y, Feng L, Ma X, Ye X.
BIOCHEM BIOPH RES CO 2013 May 17;434(4):809-14. (IF 2.985)
137. Wang Q, Li LH, Gao GD, Wang G, Qu L, Li JG, Wang CM.
Mol Biol Rep 2013 May;40(5):3723-9. (IF 1.402)
138. Yang M, Qin Z, Zhu Y, Li Y, Qin Y, Jing Y, Liu S.
Mol Neurobiol 2013 Jun;47(3):946-56. (IF 4.5)
139. Liang X, Cheng S, Jiang X, He X, Wang Y, Jiang Z, Hou W, Li S, Liu Y, Wang Z.
J BIOL RHYTHM 2013 Jun;28(3):208-17. (IF 3.122)
142. Ru R, Yao Y, Yu S, Yin B, Xu W, Zhao S, Qin L, Chen X.
Cell Regeneration 2013 Jun 18;2(1):5.
143. Li H, Zhou RJ, Zhang GQ, Xu JP.
TUMOR BIOL  2013 Jun;34(3):1807-12. (IF 3.65)
144. Liu E, Kuang Y, He W, Xing X, Gu J.
CELL PHYSIOL BIOCHEM 2013 Jun 3;31(6):805-814. (IF 5.5)
145. Zhao Y, Wang MY, Hao K, Chen XQ, Du JZ.
Peptides 2013 Jun;44:8-14. (IF 2.843)
146. Han J, Ding R, Zhao D, Zhang Z, Ma X.
Thromb Res 2013 Jul;132(1):e42-7. (IF 2.869)
147. Xue B, Wu W, Huang K, Xie T, Xu X, Zhang H, Qi C, Ge J, Yu Y.
Mol Cell Biochem 2013 Aug;380(1-2):177-84. (IF 2.795)
148. Wang J, Huang H, Wang C, Liu X, Hu F, Liu M.
INT J ORAL MAX SURG 2013 Aug;42(8):949-55. (IF 2.068)
149. Zhang J, Ni SS, Zhao WL, Dong XC, Wang JL.
TUMOR BIOL  2013 Aug;34(4):2397-401. (IF 3.65)
150. Ding F, Yu L, Wang M, Xu S, Xia Q, Fu G.
Amino Acids 2013 Aug;45(2):339-49. (IF 3.063)
151. Cong ZX, Wang HD, Wang JW, Zhou Y, Pan H, Zhang DD, Zhu L.
Oncol Rep 2013 Aug;30(2):715-22. (IF 3.417)
152. Chen Z, Yang Y, Yang X, Zhou C, Li F, Lei P, Zhong L, Jin X, Peng G.
Neurol Sci 2013 Sep;34(9):1559-70. (IF 2.415)
153. Zhang RX, Yan XB, Gu YH, Huang D, Gan L, Han R, Huang LH.
Int J Neurosci 2013 Sep;123(9):650-6. (IF 2.107)
156. Sun LL, Zhang Y, Liu JF, Wang J, Zhu WL, Zhao LY, Xue YX, Lu L, Shi J.
INT J NEUROPSYCHOPH 2013 Sep;16(8):1767-80. (IF 4.333)
157. Ge D, Kong X, Liu W, Zhao J, Su L, Zhang S, Zhang Y, Zhao B, Miao J.
Apoptosis 2013 Sep;18(9):1120-31. (IF 4.543)
158. Zheng XF, Li B, Zhang YH, Yang YH, Meng XY, Jiang SD, Jiang LS.
Menopause 2013 Oct;20(10):1074-83. (IF 3.305)
159. Mu H, Yao RB, Zhao LJ, Shen SY, Zhao ZM, Cai H.
Inflammation 2013 Oct;36(5):1136-44. (IF 3.212)
160. Fei X, Jiang S, Zhang S, Li Y, Ge J, He B, Goldstein S, Ruiz G.
Cell Biochem Biophys 2013 Nov;67(2):689-94. (IF 2.073)
162. Wu H, Wang K, Liu W, Hao Q.
BIOCHEM BIOPH RES CO 2014 Feb 7;444(2):141-6. (IF 2.985)
163. Wang C, Pan Y, Zhang R, Bai B, Chen J, Randeva HS.
BBA-BIOMEMBRANES 2014 Mar;1843(3):652-63. (IF 3.411)
164. Xie Q, Wang Z, Bi X, Zhou H, Wang Y, Gu P, Fan X.
BIOCHEM BIOPH RES CO 2014 Mar 28;446(1):98-104. (IF 2.985)
165. Cao M, Hou D, Liang H, Gong F, Wang Y, Yan X, Jiang X, Wang C, Zhang J, Zen K, Zhang CY, Chen X.
Eur J Cancer 2014 Mar;50(5):1013-24. (IF 7.275)
166. Guo S, Xu X, Tang Y, Zhang C, Li J, Ouyang Y, Ju J, Bie P, Wang H.
Cancer Lett 2014 Mar 1;344(1):40-6. (IF 7.36)
167. Peng H, Zhang W, Xiao T, Zhang Y.
REPROD FERT DEVELOP 2014 Jun;26(5):758-68. (IF 1.718)
169. Li Z, Bi X, Wang M, Zhang J, Song J, Shen X, Han J, Fu G, Ye Y.
Clin Exp Immunol 2014 Jun;176(3):429-37. (IF 3.532)
171. Wang G, Liu X, Meng L, Liu S, Wang L, Li J, Cui C, Meng J, Hu S, Wei Y.
Int J Biol Sci 2014 Jul 17;10(7):798-806. (IF 4.858)
172. Fu Y, Zhang P, Ge J, Cheng J, Dong W, Yuan H, Du Y, Yang M, Sun R, Jiang H.
INT J BIOCHEM CELL B 2014 Sep;54:68-77. (IF 3.673)
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Lab Invest 2014 Sep;94(9):1042-53. (IF 4.197)
174. Zhang X, Chen G, Lu Y, Liu J, Fang M, Luo J, Cao Q, Wang X.
Cereb Cortex 2014 Oct;24(10):2533-40. (IF 5.043)
175. Xu J, Ruan L, Shi H.
FISH SHELLFISH IMMUN 2014 Oct;40(2):609-15. (IF 3.298)
176. Qian J, Wu C, Chen X, Li X, Ying G, Jin L, Ma Q, Li G, Shi Y, Zhang G, Zhou N.
Cell Signal 2014 Nov;26(11):2412-23. (IF 3.968)
177. Li S, Wang L, Zhao Q, Liu Y, He L, Xu Q, Sun X, Teng L, Cheng H, Ke Y.
J Biol Chem 2014 Dec 5;289(49):34152-60. (IF 4.238)
178. Zhu Y, Qin Z, Gao J, Yang M, Qin Y, Shen T, Liu S.
Mol Neurobiol 2014 Dec;50(3):733-43. (IF 4.5)
181. Chen ZY, Zhang XW, Yu L, Hua R, Zhao XP, Qin X, Zhang YM.
Eur J Pain 2015 Feb;19(2):176-86. (IF 3.492)
182. Wang C, Qu B, Wang Z, Ju J, Wang Y, Wang Z, Cao P, Wang D.
Atherosclerosis 2015 Feb;238(2):201-6. (IF 3.919)
183. Zhang Y, Lv X, Bai Y, Zhu X, Wu X, Chao J, Duan M, Buch S, Chen L, Yao H.
J NEUROINFLAMM 2015 Feb 17;12:29. (IF 5.793)
184. Guo N, Meng C, Bai W, Wei Q, Shi F, Davis JS, Mao D.
Acta Histochem 2015 Mar;117(2):211-8. (IF 2.107)
185. Liu Y, Zhang Y, Jia K, Dong Y, Ma W.
Exp Ther Med 2015 Apr;9(4):1401-1406. (IF 1.785)
186. Guo F, Jiang T, Song W, Wei H, Wang F, Liu L, Ma L, Yin H, Wang Q, Xiong L.
Mol Neurobiol 2015 Apr;51(2):685-95. (IF 4.5)
188. Li G, Zhou Q, Hu Z, Wang P, Tang Q, Chen K, Yao Q.
Curr Microbiol 2015 Apr;70(4):623-9. (IF 1.746)
191. Xie Z, Liu L, Zhu W, Liu H, Wang L, Zhang J, Chen C, Zhu H.
ARTIF CELL NANOMED B 2015 Jun;43(3):180-5. (IF 3.343)
192. Gao X, Liu W, Huang L, Zhang T, Mei Z, Wang X, Gong J, Zhao Y, Xie F, Ma J, Qian L.
CELL STRESS CHAPERON 2015 Jul;20(4):653-61. (IF 2.892)
193. Chen P, Hu YF, Wang L, Xiao WF, Bao XY, Pan C, Yi HS, Chen XY, Pan MH, Lu C.
PLoS One 2015 Jul 30;10(7):e0134694. (IF 2.74)
194. Yang W, Zou Y, Zhang M, Zhao N, Tian Q, Gu M, Liu W, Shi R, Lü Y, Yu W.
Neurochem Res 2015 Aug;40(8):1576-82. (IF 3.038)
195. Zhu XC, Jiang T, Zhang QQ, Cao L, Tan MS, Wang HF, Ding ZZ, Tan L, Yu JT.
Mol Neurobiol 2015 Aug;52(1):375-85. (IF 4.5)
196. Zhao W, Sun Z, Wang S, Li Z, Zheng L.
Inflammation 2015 Aug;38(4):1700-6. (IF 3.212)
197. Zhang Y, Wu S, Song S, Lv J, Feng C, Lin X.
MOL CELL PROBE 2015 Aug;29(4):244-53. (IF 1.951)
200. Wang Y, Cui Y, Cao F, Qin Y, Li W, Zhang J.
Int Immunopharmacol 2015 Sep;28(1):136-45. (IF 3.943)
201. Li J, Li O, Kan M, Zhang M, Shao D, Pan Y, Zheng H, Zhang X, Chen L, Liu S.
Mol Med Rep 2015 Sep;12(3):4572-7. (IF 2.1)
202. Kong M, Mao J, Luo B, Qin Z.
INT J CLIN EXP PATHO 2015 Sep 1;8(9):10929-34. (IF 0.252)
203. Liu F, Zhang T, Zou S, Jiang B, Hua D.
Mol Med Rep 2015 Oct;12(4):5455-60. (IF 2.1)
204. Liu X, Dai F, Guo E, Li K, Ma L, Tian L, Cao Y, Zhang G, Palli SR, Li S.
J Biol Chem 2015 Nov 6;290(45):27370-83. (IF 4.238)
205. Chen D, Su A, Fu Y, Wang X, Lv X, Xu W, Xu S, Wang H, Wu Z.
ANTIVIR RES 2015 Nov;123:27-38. (IF 4.101)
206. Liu J, , Jin X, Zhao N, Ye X, Ying C.
J Appl Toxicol 2015 Nov;35(11):1309-17. (IF 2.997)
207. Liu R, Li X, Luo L, Qiang X, Hylemon PB, Jiang Z, Zhang L, Zhou H.
J BIOL CHEM 2015 Dec 25;290(52):30988-1002. (IF 4.238)
208. Song Y, Wan X, Gao L, Pan Y, Xie W, Wang H, Guo J.
Mol Immunol 2015 Dec;68(2 Pt A):341-9. (IF 3.641)
210. Chen D, Tao X, Wang Y, Tian F, Wei Y, Chen G, Shen H, Wang Z, Yu Z, Li H, Chen G.
Int J Mol Med 2015 Dec;36(6):1563-71. (IF 3.098)
211. Liu GY, , Jiang XX, Zhu X, He WY, Kuang YL, Ren K, Lin Y, Gou X.
Acta Pharmacol Sin 2015 Dec;36(12):1473-9. (IF 5.064)
212. Wang Y, Leng H, Chen H, Wang L, Jiang N, Huo X, Yu B.
Oncol Res 2016;24(5):361-369. (IF 4.949)
213. Zhu Y, Wei W, Ye T, Liu Z, Liu L, Luo Y, Zhang L, Gao C, Wang N, Yu L.
CELL PHYSIOL BIOCHEM 2016;40(1-2):297-308. (IF 5.5)
214. Han L, Guo X, Bian H, Yang L, Chen Z, Zang W, Yang J.
EVID-BASED COMPL ALT 2016;2016:4651949. (IF 1.813)
215. Huang X, Jin J, Shen S, Xia Y, Xu P, Zou X, Wang H, Yi L, Wang Y, Gao Q.
Gynecol Endocrinol 2016;32(3):201-5. (IF 1.571)
216. Lu J, Xu Y, Wei X, Zhao Z, Xue J, Liu P.
Biomed Res Int 2016;2016:6253280. (IF 2.276)
217. Wu T, Wang C, Ding L, Shen Y, Cui H, Wang M, Wang H.
MEDIAT INFLAMM 2016;2016:9618795. (IF 3.758)
219. Chen T, Chen N, Pang N, Xiao L, Li Y, Li R, Luo M, Deng X, Ren M, Wu J, Wang L.
J Vasc Res 2016;53(5-6):245-254. (IF 1.725)
220. Da Z, Li L, Zhu J, Gu Z, You B, Shan Y, Shi S.
J Immunol Res 2016;2016:2063985. (IF 3.327)
221. Fu J, Zhang X, Chen P, Zhang Y.
J Toxicol Sci 2016;41(6):745-756. (IF 1.737)
222. Zheng Q, Guan Y, Xia L, Wang Z, Jiang Y, Zhang X, Wang J, Wang G, Pu Y, Xia J, Luo M.
EVID-BASED COMPL ALT 2016;2016:2696480. (IF 1.813)
223. Wang H, Xue L, Li Y, Zhao B, Chen T, Liu Y, Chang L, Wang J.
FOLIA HISTOCHEM CYTO 2016;54(3):143-150. (IF 0.854)
225. Zhu D, Zhang L, Cheng L, Ren L, Tang J, Sun D.
KIDNEY BLOOD PRESS R 2016;41(1):9-17. (IF 1.898)
226. Ma C, Pan Y, Yang Z, Meng Z, Sun R, Wang T, Fei Y, Fan W.
Life Sci 2016 Jan 1;144:113-20. (IF 3.647)
227. Chen N, Ma J, Zhao Y, Wu M, Yang H, Gong W, Chao J, Li X..
APPL MICROBIOL BIOT 2016 Jan;100(2):625-35. (IF 3.53)
228. Yuan C, Zhu X, Han Y, Song C, Liu C, Lu S, Zhang M, Yu F, Peng Z, Zhou C.
J EXP CLIN CANC RES 2016 Jan 21;35:15. (IF 7.068)
229. Chang L, Wu P, Senthilkumar R, Tian X, Liu H, Shen X, Tao Z, Huang P.
J CANCER RES CLIN 2016 Jan;142(1):59-72. (IF 3.656)
230. Liu S, Chen S, Li M, Zhang B, Shen P, Liu P, Zheng D, Chen Y, Jiang J.
Arch Biochem Biophys 2016 Jan 15;590:37-47. (IF 3.391)
231. Gong T, Wei Q, Mao D, Shi F.
Acta Histochem 2016 Jan;118(1):20-30. (IF 2.107)
232. Ma WH, Liu YC, Xue ML, Zheng Z, Ge YL.
Oncol Lett 2016 Jan;11(1):159-167. (IF 2.311)
233. Long C, Wang J, Guo W, Wang H, Wang C, Liu Y, Sun X.
BIOCHEM BIOPH RES CO 2016 Jan 1;469(1):87-93. (IF 2.985)
234. Cheng S, Liang X, Wang Y, Jiang Z, Liu Y, Hou W, Li S, Zhang J, Wang Z.
EXP BIOL MED 2016 Jan;241(2):205-15. (IF 3.139)
235. Shu Y, Xiao B, Wu Q, Liu T, Du Y, Tang H, Chen S, Feng L, Long L, Li Y, 5.
Mol Neurobiol 2016 Jan;53(1):561-76. (IF 4.5)
236. Li J, Dong G, Wang B, Gao W, Yang Q.
BIOCHEM BIOPH RES CO 2016 Jan 1;469(1):15-21. (IF 2.985)
237. Ma Y, Wei Z, Bast RC Jr, Wang Z, Li Y, Gao M, Liu Y, Wang X, Guo C, Zhang L, Wang X.
Lab Invest 2016 Jan;96(1):37-48. (IF 4.197)
238. Tang X, Sun J, Du L, Du H, Wang L, Mai J, Zhang F, Liu P.
Exp Eye Res 2016 Feb;143:110-9. (IF 3.011)
239. Li J, Bian EB, He XJ, Ma CC, Zong G, Wang HL, Zhao B.
Int J Oncol 2016 Feb;48(2):723-33. (IF 3.899)
240. Chen Z, Xue J, Shen T, Mu S, Fu Q.
Int J Mol Med 2016 Feb;37(2):329-38. (IF 3.098)
241. Jiang W, Li Z, Zhao W, Chen H, Wu Y, Wang Y, Shen Z, He J, Chen S, Zhang J, Fu G.
Am J Transl Res 2016 Feb 15;8(2):329-41. (IF 3.375)
242. Xiao J, Bei Y, Liu J, Dimitrova-Shumkovska J, Kuang D, Zhou Q, Li J, Yang Y, Xiang Y, Wang F, Yang C, Yang W.
J Cell Mol Med 2016 Feb;20(2):204-16. (IF 4.486)
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Oncol Rep 2016 Feb;35(2):1034-40. (IF 3.417)
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J Mol Histol 2016 Feb;47(1):1-8. (IF 2.531)
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Arch Biochem Biophys 2016 Feb 1;591:141-9. (IF 3.391)
247. Xiong M, Wang L, Yu HL, Han H, Mao D, Chen J, Zeng Y, He N, Liu ZG, Wang ZY, Xu SJ, Guo LY, Wang YA.
Oncol Rep 2016 Feb;35(2):1034-40. (IF 3.417)
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Neural Regen Res 2016 Feb;11(2):326-31. (IF 3.171)
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Braz J Med Biol Res 2016 Feb;49(2):e4857. (IF 2.023)
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KOREAN J PHYSIOL PHA 2016 Mar;20(2):153-60. (IF 1.805)
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BIOCHEM BIOPH RES CO 2016 Mar 25;472(1):68-74. (IF 2.985)
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Int J Mol Med 2016 Mar;37(3):836-42. (IF 3.098)
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ONCOTARGETS THER 2016 Mar 11;9:1425-36. (IF 3.337)
256. Lu Y, Zhu M, Li W, Lin B, Dong X, Chen Y, Xie X, Guo J, Li M.
J Cell Mol Med 2016 Mar;20(3):549-58. (IF 4.486)
257. Zheng W, Pan S, Wang G, Wang YJ, Liu Q, Gu J, Yuan Y, Liu XZ, Liu ZP, Bian JC.
ENVIRON TOXICOL CHEM 2016 Mar;42:146-55. (IF 3.152)
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Life Sci 2016 Mar 1;148:220-8. (IF 3.647)
259. Zhu C, He L, Zhou X, Nie X, Gu Y.
Oncol Rep 2016 Mar;35(3):1318-28. (IF 3.417)
261. Cai ZZ, Xu JG, Zhou YH, Zheng JH, Lin KZ, Zheng SZ, Ye MS, He Y, Liu CB, Xue ZX.
WORLD J GASTROENTERO 2016 Mar 7;22(9):2789-98. (IF 3.665)
262. Jia J, Yang F, Yang M, Wang C, Song Y.
BIOCHEM BIOPH RES CO 2016 Mar 11;471(3):386-90. (IF 2.985)
263. Yang S, Liu J, Zhang X, Tian J, Zuo Z, Liu J, Yue X.
Am J Transl Res 2016 Mar 15;8(3):1437-46. (IF 3.375)
264. Liu W, Chen C, Chen L, Wang L, Li J, Chen Y, Jin J, Kawan A, Zhang X.
SCI REP-UK 2016 Mar 10;6:22819. (IF 3.998)
265. Chen H, Lu Y, Cao Z, Ma Q, Pi H, Fang Y, Yu Z, Hu H, Zhou Z.
Toxicol Lett 2016 Mar 30;246:7-16. (IF 3.569)
266. Zhang X, Wang Y, Han S, Xiang H, Peng Y, Wu Y, Pan S, Zhang Y, Ruan J.
PLoS One 2016 Mar 14;11(3):e0151679. (IF 2.74)
267. Hong Y, Wang X, Sun S, Xue G, Li J, Hou Y.
Int Immunopharmacol 2016 Apr;33:83-9. (IF 3.943)
268. Wang Y, Zhang B, Liu W, Dai Y, Shi Y, Zeng Q, Wang F.
ONCOTARGET 2016 Apr 19;7(16):22355-67. (IF 5.168)
269. Zhang Y, Peng Z, Zhao Y, Chen L.
MED SCI MONITOR 2016 Apr 27;22:1415-20. (IF 1.918)
270. Liu C, Qian P, Yang L, Zhang L, Chen C, He M, Lu Y, Feng W, Li M, Zhang Y, Zhong M, Yu Z, Zhou Z.
Arch Toxicol 2016 Apr;90(4):955-69. (IF 5.059)
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Immunol Res 2016 Apr;64(2):531-9. (IF 2.507)
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PLoS One 2016 Apr 25;11(4):e0153505. (IF 2.74)
275. Pan YY, Deng Y, Xie S, Wang ZH, Wang Y, Ren J, Liu HG.
CHINESE MED J-PEKING 2016 Apr 5;129(7):838-45. (IF 1.585)
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VET IMMUNOL IMMUNOP 2016 Apr;172:55-63. (IF 1.713)
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Biol Trace Elem Res 2016 Apr;170(2):340-7. (IF 2.639)
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FREE RADICAL BIO MED 2016 Apr;93:52-66. (IF 6.17)
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ONCOTARGETS THER 2016 Apr 18;9:2305-15. (IF 3.337)
280. Zhu Y, Luo M, Huang H, Du X, Chen D, Xing Q, Wang B, Cao Y.
Reprod Biomed Online 2016 Apr;32(4):388-93. (IF 3.218)
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Int J Mol Med 2016 May;37(5):1421-8. (IF 3.098)
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Oncol Rep 2016 May;35(5):2801-10. (IF 3.417)
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J Cancer 2016 May 12;7(8):935-46. (IF 3.565)
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BIOCHEM BIOPH RES CO 2016 May 20;474(1):182-7. (IF 2.985)
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Joint Bone Spine 2016 May;83(3):282-9. (IF 3.741)
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MED SCI MONITOR 2016 May 29;22:1808-16. (IF 1.918)
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BIOCHEM BIOPH RES CO 2016 May 13;473(4):913-9. (IF 2.985)
288. Guo W, Yu D, Wang X, Luo C, Chen Y, Lei W, Wang C, Ge Y, Xue W, Tian Q, Gao X, Yao W.
ONCOTARGET 2016 May 31;7(22):31800-13. (IF 5.168)
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PLoS One 2016 May 31;11(5):e0156686. (IF 2.74)
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EUR REV MED PHARMACO 2016 May;20(10):2004-10. (IF 3.024)
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Mol Immunol 2016 May;73:46-52. (IF 3.641)
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293. Hua K, Jin J, Zhao J, Song J, Song H, Li D, Maskey N, Zhao B, Wu C, Xu H, Fang L.
Int J Oncol 2016 May;48(5):1997-2006. (IF 3.899)
294. Bin G, Bo Z, Jing W, Jin J, Xiaoyi T, Cong C, Liping A, Jinglin M, Cuifang W, Yonggang C, Yayi X.
Exp Cell Res 2016 May 1;343(2):208-17. (IF 3.383)
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Molecules 2016 May 12;21(5). pii: E625. (IF 3.267)
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BIOCHEM BIOPH RES CO 2016 May 13;473(4):987-92. (IF 2.985)
297. Wang K, Wang Y, Qi Q, Zhang F, Zhang Y, Zhu X, Liu G, Luan Y, Zhao Z, Cai J, Cao J, Li S.
Int Immunopharmacol 2016 May;34:37-43. (IF 3.943)
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BMC Urol 2016 Jun 17;16(1):33. (IF 1.592)
299. Liang H, Liu S, Chen Y, Bai X, Liu L, Dong Y, Hu M, Su X, Chen Y, Huangfu L, Li X, Gu Y, Shan H.
J MOL MED 2016 Jun;94(6):655-65. (IF 4.427)
300. Yang Y, Wang Y, Wang Y, Zhao M, Jia H, Li B, Xing D.
Inflammation 2016 Jun;39(3):1151-9. (IF 3.212)
301. Duan GL, Wang CN, Liu YJ, Yu Q, Tang XL, Ni X, Zhu XY.
Endocr J 2016 Jun 30;63(6):569-80. (IF 1.952)
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PSYCHOPHARMACOLOGY 2016 Jun;233(12):2229-39. (IF 3.13)
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EUR REV MED PHARMACO 2016 Jun;20(11):2271-7. (IF 3.024)
304. Wang F, Li M, Li X, Kinden R, Zhou H, Guo F, Wang Q, Xiong L.
REJUV RES 2016 Jun;19(3):215-22. (IF 3.811)
306. Wu W, Sun Y, Zhao C, Zhao C, Chen X, Wang G, Pang W, Yang G.
ACTA BIOCH BIOPH SIN 2016 Jun;48(6):509-19. (IF 2.836)
307. Li R, Li Y, Hu X, Lian H, Wang L, Fu H.
Biochem Cell Biol 2016 Jun;94(3):247-55. (IF 2.46)
309. Zhang H, Li Z, Chu B, Zhang F, Zhang Y, Ke F, Chen Y, Xu Y, Liu S, Zhao S, Liang H, Weng M, Wu X, Li M, Wu W, Quan Z, Liu Y, Zhang Y, Gong W.
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310. Wan X, Yang R, Liu G, Zhu M, Zhang T, Liu L, Wu R.
J Vet Sci 2016 Jun 30;17(2):171-8. (IF 1.561)
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Oncol Lett 2016 Jun;11(6):3790-3794. (IF 2.311)
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314. Shen ZL, Wang B, Jiang KW, Ye CX, Cheng C, Yan YC, Zhang JZ, Yang Y, Gao ZD, Ye YJ, Wang S.
ONCOTARGET 2016 Jun 7;7(23):35092-105. (IF 5.168)
315. Tang H, Su H, Fan D, Ye C, Lei CT, Jiang HJ, Gao P, He FF, Zhang C.
AM J PHYSIOL-RENAL 2016 Jul 1;311(1):F207-16. (IF 3.144)
316. Huang W, Li J, Guo X, Zhao Y, Yuan X.
Biomed Pharmacother 2016 Jul;81:431-8. (IF 4.545)
318. Wang HB, Yan HC, Liu Y3
Clin Transl Oncol 2016 Jul;18(7):735-42. (IF 2.737)
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BIOSCI BIOTECH BIOCH 2016 Jul;80(7):1313-20. (IF 1.516)
321. Yao Y, Zhang X, Chen HP, Li L, Xie W, Lan G, Zhao ZW, Zheng XL, Wang ZB, Tang CK.
Atherosclerosis 2016 Jul;250:122-32. (IF 3.919)
322. Gao Y, Ma L, Luo CL, Wang T, Zhang MY, Shen X, Meng HH, Ji MM, Wang ZF, Chen XP, Tao LY.
Mol Neurobiol 2016 Jul 12. [Epub ahead of print] (IF 4.5)
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324. Wang Y, Hu PC, Ma YB, Fan R, Gao FF, Zhang JW, Wei L.
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J POULT SCI 2016 Jul 1;95(7):1631-5. (IF 0.88)
326. Zhou DD, Wang X, Wang Y, Xiang XJ, Liang ZC, Zhou Y, Xu A, Bi CH, Zhang L.
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327. Wang YC, Wu YN, Wang SL, Lin QH, He MF, Liu QL, Wang JH.
Int J Gynecol Cancer 2016 Jul;26(6):994-1003. (IF 2.095)
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329. Wang X, Chen J, Wang J, Yu F, Zhao S, Zhang Y, Tang H, Peng Z.
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PLoS One 2016 Jul 28;11(7):e0160042. (IF 2.74)
332. Lu YZ, Li PF, Li YZ, Luo F, Guo C, Lin B, Cao XW, Zhao J, Wang FJ.
Fitoterapia 2016 Jul;112:183-90. (IF 2.527)
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DIGEST DIS SCI 2016 Aug;61(8):2272-83. (IF 2.751)
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Biol Pharm Bull 2016 Aug 1;39(8):1300-8. (IF 1.863)
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Oncol Rep 2016 Aug;36(2):659-68. (IF 3.417)
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341. Wang Y, Zhang X, Zhong M, Liu T, Zhang G, Liu S, Guo W, Wei M, He Q, Sun D, Hu S.
Obes Surg 2016 Aug;26(8):1768-76. (IF 3.412)
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Eur J Pharmacol 2016 Aug 5;784:129-36. (IF 3.263)
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J Ovarian Res 2016 Aug 17;9(1):49. (IF 2.989)
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EUR REV MED PHARMACO 2016 Aug;20(16):3410-6. (IF 3.024)
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Int J Mol Med 2016 Aug;38(2):423-32. (IF 3.098)
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Inflammation 2016 Aug;39(4):1558-65. (IF 3.212)
349. Wang Y, Yang C, He Y, Zhan X, Xu L.
Mol Med Rep 2016 Aug;14(2):1756-64. (IF 2.1)
350. Qiu X, Zhan Y, Meng C, Wang J, Dong L, Sun Y, Tan L, Song C, Yu S, Ding C.
Arch Virol 2016 Aug;161(8):2103-16. (IF 2.243)
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Toxicol In Vitro 2016 Sep;35:112-20. (IF 2.959)
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Fitoterapia 2016 Sep;113:58-63. (IF 2.527)
353. Chen J, Gu Y, Zhang Z, Zheng W, Yang L, Huang W, Lin S, Li Y, Guo H, Luo M, Ma Q, Jiang Z, Tang A, Gui Y.
Biol Reprod 2016 Sep;95(3):58. (IF 3.322)
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TOXICOL APPL PHARM  2016 Sep 1;306:58-68. (IF 3.616)
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Gene 2016 Sep 1;589(1):12-9. (IF 2.984)
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Cancer Sci 2016 Sep;107(9):1215-22. (IF 4.966)
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Cell Signal 2016 Sep;28(9):1163-71. (IF 3.968)
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COLLOID SURFACE B 2016 Sep 1;145:37-45. (IF 4.389)
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Mol Med Rep 2016 Sep;14(3):2321-7. (IF 2.1)
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Cancer Lett 2016 Sep 28;380(1):20-30. (IF 7.36)
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J EXP CLIN CANC RES 2016 Sep 30;35(1):159. (IF 7.068)
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SCI REP-UK 2016 Oct 21;6:35489. (IF 3.998)
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Drug Chem Toxicol 2016 Oct;39(4):418-23. (IF 2.405)
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NEUROSIGNALS  2016 Oct 24;24(1):95-101. (IF 6.143)
371. Mo SF, Liao GY, Yang J, Wang MY, Hu Y, Lian GN, Kong LD, Zhao Y.
Brain Res 2016 Oct 1;1648(Pt A):250-6. (IF 2.733)
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Exp Ther Med 2016 Oct;12(4):2741-2746. (IF 1.785)
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J Transl Med 2016 Oct 26;14(1):303. (IF 4.124)
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Connect Tissue Res 2016 Oct 20:1-8. (IF 2.795)
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PLoS One 2016 Nov 28;11(11):e0166560. (IF 2.74)
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J Headache Pain 2016 Dec;17(1):90. (IF 4.797)
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J Ethnopharmacol 2016 Dec 4;193:368-376. (IF 3.69)
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