BCA蛋白浓度测定试剂盒(增强型)
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产品编号 ： P0010
数量 ：
¥ 375.00 元
产品包装:
5000次 500次 200次
产品简介
使用说明
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BCA蛋白浓度测定试剂盒(增强型)(Enhanced BCA Protein Assay Kit)是根据目前世界上最常用的两种蛋白浓度检测方法之一BCA法研制而成，实现了蛋白浓度测定的简单、高稳定性、高灵敏度和高兼容性。
和碧云天生产的普通BCA蛋白浓度测定试剂盒相比，灵敏度更高，检测浓度下限达到10μg/ml，最小检测蛋白量达到
0.2μg，待测样品体积为1-20μl。
和碧云天生产的普通BCA蛋白浓度测定试剂盒相比，显色速度更快，相同的样品孵育较短时间即可进行吸光度测定。
在20-1000μg/ml浓度范围内有较好的线性关系。本产品从0.025到0.5mg/ml的标准曲线参考图1。

图1. 本试剂盒蛋白标准曲线的效果图。左上图为加入BCA工作液后37℃分别孵育30、60和120分钟后的吸光度实测效果图，右图为37℃孵育60分钟时的实拍显色效果图。图中数据仅供参考，实际的检测效果可能会略有不同。

BCA法测定蛋白浓度不受绝大部分样品中的化学物质的影响，可以兼容样品中高达5%的SDS，5%的Triton X-100，5%的
Tween 20、60、80。但本试剂盒受螯合剂和略高浓度的还原剂的影响，需确保EDTA低于10mM，无EGTA，二硫苏糖醇(DTT)低于1mM，β-巯基乙醇(β-Mercaptoethanol)低于0.01%。不适用BCA法时建议试用碧云天生产的Bradford蛋白浓度测定试剂盒(P0006)。
BCA蛋白浓度测定试剂盒(增强型)对样品中各种物质的详细的兼容性和普通的BCA蛋白浓度测定试剂盒相同，请参考碧云天如下网页：http://www.beyotime.com/Compatibility Chart For BCA Kit.pdf
每个试剂盒可以检测500个样品。
包装清单：

产品编号 产品名称 包装
P0010-1 BCA试剂 A 100ml
P0010-2 BCA试剂 B 3ml
P0010-3 蛋白标准(BSA) 30mg
P0010-4 蛋白标准配制液 1.5ml
— 说明书 1份

保存条件：
室温保存。蛋白标准配制成溶液后-20℃冻存。
注意事项：
需酶标仪一台，测定波长为540-595nm之间，562nm最佳。需96孔板。如果没有酶标仪，也可以使用普通的分光光度计测定，但测定时，需根据比色皿的最小检测体积，适当加大BCA工作液的用量使不小于最小检测体积，样品和标准品的用量可相应按比例放大也可不变。使用分光光度计测定蛋白浓度时，每个试剂盒可以测定的样品数量可能会显著减少。
如发现样品稀释液或裂解液本身就有较高背景，请试用碧云天生产的Bradford蛋白浓度测定试剂盒(P0006)。
为了加快BCA法测定蛋白浓度的速度可以适当用微波炉加热，但是切勿过热。
EDTA浓度必须小于10mM，不兼容EGTA。不适用BCA法时，请试用碧云天生产的Bradford蛋白浓度测定试剂盒(P0006)。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1.蛋白标准品的准备
a.取1.2ml蛋白标准配制液加入到一管蛋白标准(30mg BSA)中，充分溶解后配制成25mg/ml的蛋白标准溶液。 配制后可立即使用，也可以-20℃长期保存。
b.取适量25mg/ml蛋白标准，稀释至终浓度为0.5mg/ml。例如取20μl 25mg/ml蛋白标准，加入980μl稀释液即可配制成0.5mg/ml蛋白标准。蛋白样品在什么溶液中，标准品也宜用什么溶液稀释。但是为 了简便起见，也可以用0.9%NaCl或PBS稀释标准品。稀释后的0.5mg/ml蛋白标准可以-20℃长期保存。
2.BCA工作液的配制
根据样品数量，按50体积BCA试剂A加1体积BCA试剂B(50:1)配制适量BCA工作液，充分混匀。例如5mlBCA试剂A 加100μl BCA试剂B，混匀，配制成5.1ml BCA工作液。 BCA工作液室温24小时内稳定。
3.蛋白浓度检测
a.将标准品按0、1、2、4、8、12、16、20μl加到96孔板的标准品孔中，加标准品稀释液补足到20μl，相当于标准品浓度分别为0、0.025、0.05、0.1、0.2、0.3、0.4、0.5mg/ml。
b.加适当体积样品到96孔板的样品孔中。如果样品不足20μl，加标准品稀释液补足到20μl。请注意记录样品体积。
c.各孔加入200μl BCA工 作液，37℃放置20-30分钟。
注:也可以室温放置2小时，或60℃放置30分钟。BCA法测定蛋白浓度时，颜色会随着时间的延长不断加深。并且显色反应会因温 度升高而加快。如果浓度较低，适合在较高温度孵育，或适当延长孵育时间。
d.用酶标仪测定A562，或540-595nm之间的波长的吸光度。
e.根据标准曲线和使用的样品体积计算出样品的蛋白浓度。
常见问题：
1.测定标准曲线时发现随着标准品浓度的增加吸光度或颜色没有明显变化。
可能的原因是样品中含有严重干扰BCA法测定蛋白浓度的物质，详细的BCA法的兼容性列表请参考碧云天如下网页:http://www.beyotime.com/Compatibility Chart For BCA Kit.pdf
2.是否每次测定时都需要做标准曲线？
建议每次测定时都做标准曲线。因为BCA法测定时颜色会随着时间的延长不断加深，并且显色反应的速度和温度有关，所以除非 精确控制显色反应的时间和温度，否则如需精确测定宜每次都做标准曲线。

相关产品：

产品编号 产品名称 包装
P0006 Bradford蛋白浓度测定试剂盒 1000次
P0006C Braford蛋白浓度测定试剂盒(去垢剂兼容型) 800次
P0007 蛋白标准(5mg/ml BSA) 1ml
P0009 BCA蛋白浓度测定试剂盒(增强型) 5000次
P0010 BCA蛋白浓度测定试剂盒(增强型) 500次
P0010S BCA蛋白浓度测定试剂盒(增强型) 200次
P0011 BCA蛋白浓度测定试剂盒 5000次
P0012 BCA蛋白浓度测定试剂盒 500次
P0012S BCA蛋白浓度测定试剂盒 200次
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424. Ma J, Li R, Wang H, Li D, Wang X, Zhang Y, Zhen W, Duan H, Yan G, Li Y.
Front Plant Sci 2017 Apr 21;8:592. (IF 4.402)
425. Li RJ, Gao CY, Guo C, Zhou MM, Luo J, Kong LY.
Inflammation 2017 Apr;40(2):401-413. (IF 3.212)
426. Li RJ, Gao CY, Guo C, Zhou MM, Luo J, Kong LY.
Inflammation 2017 Apr;40(2):401-413. (IF 3.212)
427. Zan J, Liu S, Sun DN, Mo KK, Yan Y, Liu J, Hu BL, Gu JY, Liao M, Zhou JY.
FRONT CELL INFECT MI 2017 Apr 26;7:146. (IF 4.123)
428. Qi Y, Ruan J, Wang M, Dai Y, Zhou Q, Gui S, Zhang S, Wang Y.
Biomed Rep. 2017 Apr;6(4):475-479.
429. Han Y, Wu JZ, Shen JZ, Chen L, He T, Jin MW, Liu H.
SCI REP-UK 2017 Apr 25;7(1):1123. (IF 3.998)
430. Zhou Q, Deng Q, Hu B, Wang YJ, Chen JL, Cui JJ, Cao S, Song HN.
Exp Ther Med 2017 May;13(5):2293-2303. (IF 1.785)
431. Zhang J, Zhang Z, Bao J, Yu Z, Cai M, Li X, Wu T, Xiang J, Cai D.
J Ethnopharmacol 2017 May 5;203:69-79. (IF 3.69)
432. Chen S, Lv X, Hu B, Shao Z, Wang B, Ma K, Lin H, Cui M.
Apoptosis 2017 May;22(5):626-638. (IF 4.543)
433. Xu Z, Diao Z, Liu R, Liu W.
Mol Med Rep 2017 May;15(5):2560-2566. (IF 2.1)
434. Li Z, Gu J, Liu J, Zhu Q, Lu H, Lu Y, Rao J, Lu L, Wang X.
Int Immunopharmacol 2017 May;46:201-209. (IF 3.943)
435. Huang H, Wang J, Zhang J, Luo Z, Li D, Qiu X, Peng Y, Xu Z, Xu P, Xu Z.
ONCOTARGET 2017 May 30;8(22):35573-35582. (IF 5.168)
436. Lu Z, Xiao Y, Liu X, Zhang Z, Xiao F, Bi Y.
Mol Med Rep 2017 May;15(5):2415-2422. (IF 2.1)
437. Li BY, Gao YH, Pei JR, Yang YM, Zhang W, Sun DJ.
Int J Mol Med 2017 May;39(5):1155-1163. (IF 3.098)
438. Han T, Shu T, Dong S, Li P, Li W, Liu D, Qi R, Zhang S, Zhang L.
Oncol Lett 2017 May;13(5):2982-2988. (IF 2.311)
439. Li X, Wu Y, Zhang W, Gong J, Cheng Y.
Biomed Pharmacother 2017 May;89:1392-1400. (IF 4.545)
442. Guo T, Lin Q, Li X, Nie Y, Wang L, Shi L, Xu W, Hu T, Guo T, Luo F.
J AGR FOOD CHEM 2017 May 10;65(18):3647-3658. (IF 4.192)
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BMC Vet Res 2017 Jun 5;13(1):157. (IF 1.835)
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Oncol Rep 2017 Jun;37(6):3475-3483. (IF 3.417)
445. Cao L, Huang B, Fu X, Yang J, Lin Y, Lin F.
Mol Med Rep 2017 Jun;15(6):4247-4252. (IF 2.1)
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Theranostics 2017 Jun 25;7(10):2634-2648. (IF 8.579)
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ONCOTARGET 2017 Jun 22;8(33):54838-54857. (IF 5.168)
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Environ Pollut 2017 Jun;225:47-54. (IF 6.792)
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CHINESE MED J-PEKING 2017 Jun 5;130(11):1352-1360. (IF 1.585)
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Oncol Lett 2017 Jun;13(6):4413-4419. (IF 2.311)
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Fitoterapia 2017 Jun;119:158-167. (IF 2.527)
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Exp Ther Med 2017 Jun;13(6):3003-3008. (IF 1.785)
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Cell Biol Int 2017 Jun;41(6):630-638. (IF 2.571)
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Iran J Basic Med Sci 2017 Jul;20(7):813-821. (IF 2.146)
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J Anat 2017 Jul;231(1):129-139. (IF 2.013)
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Biomed Pharmacother 2017 Jul;91:964-969. (IF 4.545)
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BIOCHEM BIOPH RES CO 2017 Jul 1;488(3):554-561. (IF 2.985)
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PARASITE VECTOR 2017 Aug 10;10(1):383. (IF 2.824)
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Behav Brain Res 2017 Aug 30;333:286-294. (IF 2.977)
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BIOCHEM BIOPH RES CO 2017 Aug 26;490(3):1132-1138. (IF 2.985)
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Life Sci 2017 Aug 1;182:10-21. (IF 3.647)
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SCI REP-UK 2017 Sep 12;7(1):11424. (IF 3.998)
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J ANIM SCI BIOTECHNO 2017 Sep 1;8:72. (IF 4.167)
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Andrology 2017 Sep;5(5):1007-1015. (IF 3.106)
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Sleep Breath 2017 Sep;21(3):667-677. (IF 2.162)
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Mol Nutr Food Res 2017 Sep;61(9). (IF 5.309)
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Oncol Lett 2017 Oct;14(4):4078-4084. (IF 2.311)
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MAT SCI ENG C-MATER 2017 Oct 1;79:507-515. (IF 5.88)
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Virol J 2017 Oct 6;14(1):193. (IF 2.579)
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EXP ANIM TOKYO 2017 Oct 30;66(4):303-312. (IF 1.574)
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Mycopathologia 2017 Oct 23. (IF 2.452)
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Cell Death Dis 2017 Oct 26;8(10):e3155. (IF 6.304)
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CHEM-BIOL INTERACT 2017 Oct 1;276:105-112. (IF 3.723)
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Ecotoxicology 2017 Oct;26(8):1078-1088. (IF 2.535)
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Chemosphere 2017 Oct;185:618-627. (IF 5.778)
509. Li Z, Lu H, Gu J, Liu J, Zhu Q, Lu Y, Wang X.
Inflammation 2017 Oct;40(5):1576-1588. (IF 3.212)
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Mol Med Rep 2017 Oct;16(4):4119-4126. (IF 2.1)
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Cell Commun Signal 2017 Oct 10;15(1):39. (IF 4.344)
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Neuroscience 2017 Oct 24;362:13-24. (IF 3.056)
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Mol Med Rep 2017 Oct;16(4):4089-4094. (IF 2.1)
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Exp Mol Pathol 2017 Oct;103(2):210-217. (IF 2.28)
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Mol Med Rep 2017 Oct;16(4):4127-4132. (IF 2.1)
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J Cancer 2017 Oct 17;8(18):3707-3717. (IF 3.565)
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Mol Med Rep 2017 Oct;16(4):5471-5479. (IF 2.1)
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Mol Med Rep 2017 Oct;16(4):5225-5234. (IF 2.1)
524. Li J, Liu B, Cai M, Lin X, Lou S.
Behav Brain Res 2017 Nov 4. pii: S0166-4328(17)31309-8. (IF 2.977)
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Mol Cell Proteomics 2017 Nov 29. pii: mcp.RA117.000159. (IF 4.87)
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Int J Oncol 2017 Nov;51(5):1439-1448. (IF 3.899)
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BIOCHEM BIOPH RES CO 2017 Nov 4;493(1):487-493. (IF 2.985)
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Exp Ther Med 2017 Nov;14(5):4194-4200. (IF 1.785)
529. Wang YL, Shao X, Wang F, Zeng L, Hu L, Cui SQ, Hou G, Huang DN.
Oncol Lett 2017 Nov;14(5):5927-5933. (IF 2.311)
530. Huang C, Li N, Yuan S, Ji X, Ma M, Rao K, Wang Z.
Environ Pollut 2017 Nov;230:775-786. (IF 6.792)
531. Wang H, Zhu L, Feng X, Zhang H, Luo Q, Chen F.
Eur J Pharmacol 2017 Nov 15;815:282-289. (IF 3.263)
532. Huo R, Zeng B, Zeng L, Cheng K, Li B, Luo Y, Wang H, Zhou C, Fang L, Li W, Niu R, Wei H, Xie P.
FRONT CELL INFECT MI 2017 Nov 30;7:489. (IF 4.123)
533. Chen R, Xu L, Fan Q, Li M, Wang J, Wu L, Li W, Duan J, Chen Z.
Drug Deliv 2017 Nov;24(1):1191-1203. (IF 4.902)
534. Kang M, Shi J, Peng N, He S.
ONCOTARGETS THER 2017 Nov 28;10:5667-5675. (IF 3.337)
535. Wang B, Lian YJ, Su WJ, Peng W, Dong X, Liu LL, Gong H, Zhang T, Jiang CL, Wang YX.
Brain Behav Immun 2017 Nov 28. pii: S0889-1591(17)30518-4. (IF 6.633)
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J Physiol Biochem 2017 Nov;73(4):583-594. (IF 2.952)
537. Zhang B, Shang P, Tao Z, Qiangba Y, Wang Z, Zhang H.
Gene 2017 Nov 15;634:68-73. (IF 2.984)
538. Yang YS, Yuan Y, Hu WP, Shang QX, Chen LQ.
SCAND J GASTROENTERO 2017 Nov 24:1-8. (IF 2.13)
539. Guo J, Guo S, Wang Y, Yu Y.
Mol Med Rep 2017 Nov;16(5):7297-7304. (IF 2.1)
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Life Sci 2017 Nov 1;188:37-44. (IF 3.647)
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BIOCHEM BIOPH RES CO 2017 Nov 4;493(1):373-381. (IF 2.985)
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Mol Med Rep 2017 Nov;16(5):6934-6939. (IF 2.1)
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Biomed Pharmacother 2017 Nov;95:1684-1692. (IF 4.545)
544. Zhao Y, He L, Zhang Y, Zhao J, Liu Z, Xing F, Liu M, Feng Z, Li W, Zhang J.
J STEROID BIOCHEM 2017 Nov;174:96-113. (IF 3.813)
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Exp Ther Med 2017 Nov;14(5):4431-4437. (IF 1.785)
546. Wu X, Liang M, Yang Z, Su M, Yang B.
ENVIRON SCI POLLUT R 2017 Nov;24(31):24201-24206. (IF 3.056)
547. Guo M, Zhao X, Yuan X, Li P.
J Hum Hypertens 2017 Dec;31(12):815-820. (IF 2.26)
548. Li S, Zhao H, Wang Y, Shao Y, Li J, Liu J, Xing M.
Chemosphere 2017 Dec;189:489-497. (IF 5.778)
549. Fu X, Xie W, Song X, Wu K, Xiao L, Liu Y, Zhang L.
CLIN RES HEPATOL GAS 2017 Dec;41(6):687-692. (IF 2.718)
550. Zhang W, Wei R, Zhang L, Tan Y, Qian C.
Neuroscience 2017 Dec 16;366:95-104. (IF 3.056)
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Mol Med Rep 2017 Dec;16(6):9095-9101. (IF 2.1)
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Mol Med Rep 2017 Dec;16(6):9125-9130. (IF 2.1)
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Exp Ther Med 2017 Dec;14(6):5889-5895. (IF 1.785)
554. Ye W, Zhang C, Xu N, Sun Y, Ma L, Shen B, Zhou D, Zhu C.
ARCH INSECT BIOCHEM 2017 Dec;96(4). (IF 1.536)
555. Zheng F, Yang Y, Lu S, Yang Q, Li Y, Xu X, Zhang Y, Liu F, Tian X, Wang X.
Neuroscience 2017 Dec 26;367:110-120. (IF 3.056)
558. Li R, Luo X, Zhu Y, Zhao L, Li L, Peng Q, Ma M, Gao Y.
Environ Pollut 2017 Dec;231(Pt 2):1560-1568. (IF 6.792)
559. Zhang D, Xu XL, Li F, Sun HC, Cui YQ, Liu S, Xu PY.
Oncol Lett 2017 Dec;14(6):8042-8050. (IF 2.311)
560. Zhou J, Zhu YF, Chen XY, Han B, Li F, Chen JY, Peng XL, Luo LP, Chen W, Yu XP.
Int J Mol Med 2017 Dec;40(6):1649-1656. (IF 3.098)
561. Liu S, Yi F, Cheng W, Qu X, Wang C.
Exp Ther Med 2017 Dec;14(6):5497-5502. (IF 1.785)
562. Li X, Wu Z, Zhang Y, Xu Y, Han G, Zhao P.
Front Mol Neurosci 2017 Dec 22;10:432. (IF 4.057)
565. Jiang KL, Zhong L, Yang XQ, Ma PP, Wang H, Zhu XY, Liu BZ.
Oncol Lett 2017 Dec;14(6):7091-7098. (IF 2.311)
566. Zhao X, Xing F, Cong Y, Zhuang Y, Han M, Wu Z, Yu S, Wei H, Wang X, Chen G.
INT J BIOCHEM CELL B 2017 Dec;93:32-40. (IF 3.673)
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Anatol J Cardiol 2017 Dec;18(6):384-390. (IF 1.223)
568. Wang N, Yang L, Zhang H, Lu X, Wang J, Cao Y, Chen L, Wang X, Cong L, Li J, Wang N, Liu Z, Wang L.
CELL PHYSIOL BIOCHEM 2017 Dec 22;45(1):78-87. (IF 5.5)
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CELL PHYSIOL BIOCHEM. 2018;46(5):2090-2102. (IF 5.5)
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J Cancer Res Ther. 2018;14(7):1519-1524. (IF 1.326)
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Yonsei Med J. 2018 Jan;59(1):43-50. (IF 1.914)
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PROSTAG OTH LIPID M. 2018 Jan;134:114-122. (IF 2.283)
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Exp Ther Med. 2018 Jan;15(1):562-568. (IF 1.785)
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Oxid Med Cell Longev. 2018 Jan 11;2018:3853409. (IF 5.076)
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Front Cell Neurosci 2018 Jan 11;11:432. (IF 3.921)
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Int Immunopharmacol 2018 Jan 20;56:71-77. (IF 3.943)
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J Mol Neurosci 2018 Jan;64(1):144-155. (IF 2.678)
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Int Urol Nephrol 2018 Feb;50(2):263-273. (IF 1.843)
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EVID-BASED COMPL ALT. 2018 Mar 12;2018:6719460. (IF 1.813)
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Int Heart J. 2018 Mar 30;59(2):394-398. (IF 1.906)
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Mol Med Rep 2018 Mar;17(3):4062-4066. doi: 10.3892/mmr.2017.8364. Epub 2017 Dec 27. (IF 2.1)
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P0012
500次
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BCA蛋白浓度测定试剂盒(增强型)
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100ml
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PMSF (100mM)
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