pLenti-DCLK3-sgRNA (DCLK3基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。
碧云天同时提供基于CRISPR/Cas9技术的DCLK3基因敲除的质粒(L15440 pLenti-DCLK3-sgRNA)、慢病毒(L15441 DCLK3 Knockout Lentivirus)、HEK293T细胞(L15442 DCLK3 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L15443 DCLK3 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L15444 DCLK3 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
DCLK3基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | DCLK3 | 85443 | - | NM_033403 |
| About the gene | |
| Official Symbol | DCLK3 |
| Previous Symbol | DCAMKL3 |
| Official Full Name | doublecortin like kinase 3 |
| Synonyms | KIAA1765; DCDC3C |
| Location | 3p22.2 |
| Gene Type | protein_coding |
| Uniprot ID | Q9C098 |
| Pathway/Library | Kinases Library |
| Gene Summary | DCLK3 is the third member of the doublecortin-like kinase (DCLK) family. The DCLK1/2 proteins have a conserved C-terminal ‘serine/threonine kinase’ domain similar to that of Ca2+/calmodulin kinase IV (CaMKIV) and an N-terminal putative doublecortin (DCX) domain mediating their anchorage to microtubules. DCLK1 and DCLK2 are involved in neural development and the maturation of neuronal architecture, including the organization of dendritic spines in particular and play an important role in axonal regeneration mainly through their DCX domain. DCLK3 functions are likely not similar to those of DCLK1/2. The putative DCX domain in the N-terminal part of DCLK1/2 is not present in DCLK3. In addition, DCLK1 and DCLK2 have only 56–54% identities in the amino acid primary sequence of DCLK3, with differences in and around the kinase domain in the C-terminal part of the proteins. DCLK3 displays a more neuron-specific pattern of expression than DCLK1/2, which are also expressed in astrocytes. DCLK3 is mainly expressed in the adult and not in the developing forebrain. DCLK3 was as an oncogenic factor in colorectal cancer. High expression of DCLK3 (resulting from at-distance effects of a single polymorphism in the promoter of the MLH1 gene) in cancer cells was shown to be associated with increased malignancy. This suggested that DCLK3 has biological functions that may modulate cell survival. The kinase activity of DCLK3 could impact striatal neurons. |
| 产品编号 | 产品名称 | 包装 |
| L15440 | pLenti-DCLK3-sgRNA | 5µg |
| — | 说明书 | 1份 |
-20℃保存，至少两年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA与质粒及其序列信息，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
慢病毒包装使用的包装质粒，可以订购碧云天的Lentivirus Packaging Vectors Set A (L00002)，包括pMDLg、Rev和VSV-g。
对于非目录产品的CRISPR基因敲除用的sgRNA表达质粒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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