pLenti-C19orf48-sgRNA (C19orf48基因敲除质粒)是一种在动物细胞中可以同时表达Cas9、目的基因的sgRNA和puromycin抗性基因的质粒。用于在动物细胞中直接基于CRISPR/Cas9技术敲除目的基因，或者通过包装慢病毒后基于CRISPR/Cas9技术敲除目的基因。本质粒中sgRNA的有效性已经通过T7EI法的验证。
本质粒在细菌中为Amp抗性，全长约13,000bp。本质粒的关键图谱信息请参考图1。本质粒可直接转染细胞用于目的基因的CRISPR/Cas9敲除，以及通过puromycin筛选稳定细胞株；也可以与pMDLg、Rev及VSV-g共转HEK293T细胞进行重组慢病毒(lentivirus)的包装，然后再用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 表达sgRNA、Cas9和puromycin抗性的pLenti-sgRNA质粒关键图谱信息。
本质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本质粒用于实验时，建议同时选购无任何靶向的对照质粒pLenti-Control-sgRNA (L00011)或靶向GFP的对照质粒pLenti-GFP-sgRNA (L00013)。
碧云天同时提供基于CRISPR/Cas9技术的C19orf48基因敲除的质粒(L12575 pLenti-C19orf48-sgRNA)、慢病毒(L12576 C19orf48 Knockout Lentivirus)、HEK293T细胞(L12577 C19orf48 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L12578 C19orf48 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L12579 C19orf48 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
C19orf48基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | C19orf48 | 84798 | BC006151 | NM_032712 |
| About the gene | |
| Official Symbol | C19orf48 |
| Previous Symbol | - |
| Official Full Name | chromosome 19 open reading frame 48 |
| Synonyms | MGC13170; BC006151 |
| Location | 19q13.33 |
| Gene Type | protein-coding gene |
| Uniprot ID | Q6RUI8 |
| Pathway/Library | Lung Cancer Growth Related Genes Library |
| Gene Summary | C19orf48 as the gene encoding a novel minor H antigen presented by HLA-A*0201 and recognized by CTL clones isolated from two patients with metastatic RCC who were treated with nonmyeloablative HLA-identical allogeneic HCT. The C19orf48 gene, first identified by the Mammalian Gene Collection Program, was subsequently cloned from a cisplatin-resistant human lung adenocarcinoma cell line. Sequence analysis of C19orf48 transcripts predicted a 117-residue translation product encoded by the largest ORF that is located in both the NM_199250 and NM_199249 transcripts. The studies of T cell recognition of C19orf48 provide direct evidence for a translation product derived from a novel ORF in the +2 frame of NM_199250 that encodes a predicted polypeptide of only 48 residues. Although differential splicing of 5 exons result in the alternative C19orf48-derived transcripts NM_199249 or NM_199250 that are discordant for the ORF encoding the antigenic 48-residue protein, our analyses of transcript expression by both northern blot and Q-PCR demonstrate that the NM_199250 transcript encoding the antigenic 48 residue polypeptide is the dominant transcript, with expression levels that are 100- to 600-fold greater than the expression levels of NM_199249. Discovery of the C19orf48-encoded minor H antigen within an unrecognized ORF adds to a growing body of data demonstrating that minor H peptides can be derived from surprisingly short protein species encoded by unconventional or occult ORFs. The C19orf48 genomic locus lies between the androgen-regulated testicular acid phosphatase (ACPT) gene and the androgen-regulated kallikrein gene cluster on chromosome 19q. |
| 产品编号 | 产品名称 | 包装 |
| L12575 | pLenti-C19orf48-sgRNA | 5µg |
| — | 说明书 | 1份 |
-20℃保存，至少两年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA与质粒及其序列信息，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
慢病毒包装使用的包装质粒，可以订购碧云天的Lentivirus Packaging Vectors Set A (L00002)，包括pMDLg、Rev和VSV-g。
对于非目录产品的CRISPR基因敲除用的sgRNA表达质粒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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