TRIM65 Knockout Lentivirus(TRIM65基因敲除慢病毒)是一种感染动物细胞后可以同时表达Cas9、目的基因sgRNA和puromycin抗性基因的慢病毒。本产品用于在动物细胞中基于CRISPR/Cas9技术敲除目的基因，并且本慢病毒中sgRNA的有效性已经通过T7EI法的验证。
本慢病毒基因序列的关键图谱信息请参考图1。本慢病毒可用于感染细胞或组织并进行目的基因的CRISPR/Cas9敲除。
图1. 可同时表达sgRNA、Cas9和puromycin抗性的本慢病毒其基因序列的关键图谱信息。
用于包装本慢病毒的质粒中的sgRNA基于碧云天研发的CRISPR/Cas9 sgRNA快速筛选和验证体系获得，sgRNA的有效性已经通过T7EI法验证。
本慢病毒用于实验时，建议同时选购无任何靶向的对照慢病毒Control Knockout Lentivirus (L00015)或靶向GFP的对照慢病毒GFP Knockout Lentivirus (L00017)。
碧云天同时提供基于CRISPR/Cas9技术的TRIM65基因敲除的质粒(L10970 pLenti-TRIM65-sgRNA)、慢病毒(L10971 TRIM65 Knockout Lentivirus)、HEK293T细胞(L10972 TRIM65 Knockout HEK293T Cells)、HEK293T敲除细胞的RIPA裂解液(L10973 TRIM65 Knockout HEK293T RIPA Lysate)、HEK293T敲除细胞的Trizol裂解液(L10974 TRIM65 Knockout HEK293T Trizol Lysate)等产品，具体请在碧云天网站查询或在本产品网页点击相应产品。
TRIM65基因的基本信息如下：
| Species | Gene Symbol | Gene ID | GenBank Accession | Transcript |
| Human | TRIM65 | 201292 | BC013181 | NM_173547 |
| About the gene | |
| Official Symbol | TRIM65 |
| Previous Symbol | - |
| Official Full Name | tripartite motif containing 65 |
| Synonyms | - |
| Location | 17q25.1 |
| Gene Type | protein-coding gene |
| Uniprot ID | Q6PJ69 |
| Pathway/Library | Ubiquitin Ligases Genes Library |
| Gene Summary | Tripartite motif (TRIM) proteins have been increasingly appreciated as important antiviral factors that suppress the replication of a wide range of RNA and DNA viruses. TRIM proteins inhibit viral replication by either directly targeting viral components or modulating innate immune responses that result in antiviral gene expression. For example, TRIM19 (also known as promyelocytic leukemia protein (PML)) restricts multiple RNA viruses and DNA viruses, including herpesviruses, by organizing PML nuclear bodies. Several TRIM proteins that suppress KSHV reactivation. TRIM65 belongs to the tripartite motif family. Originally identified as a gene with SNPs associated with cerebral white matter lesions , TRIM65 was later shown to be a cofactor for the regulation of microRNA function. TRIM65 regulates microRNA activity by ubiquitination of TNRC6 while establishing its E3 ubiquitin ligase activity. Like other TRIM members that are involved in the immune response, TRIM65 also participates in the virus-induced innate immune response by ubiquitination of substrate proteins. In tumor malignancies, TRIM65 was first shown to harbor oncogenic activities in lung and liver cancers, and cancer-related TRIM65 substrates, including TP53 and AXIN1, had been reported. More recently, TRIM65 was reported to support bladder urothelial carcinoma cell progression by targeting ANXA2 for ubiquitination and degradation. |
| 产品编号 | 产品名称 | 包装 |
| L10971 | TRIM65 Knockout Lentivirus | 108 TU |
| — | 说明书 | 1份 |
-80℃保存，至少一年有效。
注意事项：碧云天拥有sgRNA序列的知识产权，如果需要sgRNA序列，请在订购后发送邮件向info@beyotime.com索取。sgRNA序列信息与本慢病毒，未经碧云天书面许可不得用于任何商业用途，也不得移交给订货人所在实验室外的任何个人或单位。使用者在发表研究论文或结果时，应注明来源。
对于非目录产品的CRISPR基因敲除用的慢病毒的定制，可联系碧云天技术服务service@beyotime.com。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。
| 产品编号 | 产品名称 | 包装 |
| L00015 | Control Knockout Lentivirus | 108 TU |
| L00017 | GFP Knockout Lentivirus | 108 TU |
| C0222 | 青霉素-链霉素溶液(100X) | 100ml |
| C0351-1ml | Polybrene (Hexadimethrine Bromide) | 1ml |
| C0351-50mg | Polybrene (Hexadimethrine Bromide) | 50mg |
| D0508S/M | 基因组编辑突变检测试剂盒 | 25/100次 |
| D7080S/M/L | T7 Endonuclease I (CRISPR等基因突变鉴定用) | 250/1250/5000U |
| ST551-10mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×1ml |
| ST551-50mg | Puromycin Dihydrochloride (嘌呤霉素) | 10mg/ml×5ml |
| ST551-250mg | Puromycin Dihydrochloride (嘌呤霉素) | 250mg |
| ST1380-500mg | Polybrene (≥94%, Reagent grade) | 500mg |
| ST1380-2g | Polybrene (≥94%, Reagent grade) | 2g |
| ST1380-10g | Polybrene (≥94%, Reagent grade) | 10g |
