Caspase 3 活性检测试剂盒
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产品编号 ： C1115
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¥ 530.00 元
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Caspase 3 活性检测试剂盒(Caspase 3 Activity Assay Kit)是采用分光光度法检测细胞或组织裂解液中caspase 3酶活性或纯化的caspase 3酶活性的试剂盒。
Caspase(Cysteine-requiring Aspartate Protease)是一个在细胞凋亡过程中起重要作用的蛋白酶家族。Caspase 3也称
CPP32、Yama或apopain，有时被写作caspase-3或caspase 3，属于caspase家族的CED-3亚家族(CED-3 subfamily)，是细胞凋亡过程中的一个关键酶。Caspase 3是哺乳动物细胞中研究最多的一个caspase。Caspase 3可以剪切procaspase 3、6、7和9，并可以直接特异性剪切许多caspase底物，包括PARP(poly(ADP-ribose)polymerase)，ICAD(Inhibitor of caspase-activated deoxyribonuclease)，gelsolin和fodrin等。这些由caspase 3介导的蛋白剪切是细胞凋亡分子机制的重要组成部分。另外，caspase 3在细胞核凋亡过程中也起到了关键作用，包括染色质固缩(chromatin condensation)，DNA片段化(DNA fragmentation)等。同时caspase 3对细胞起泡(cell blebbing)也起到关键作用。
本Caspase 3 活性检测试剂盒是基于caspase 3可以催化底物Ac-DEVD-pNA(acetyl-Asp-Glu-Val-Asp p-nitroanilide)产生黄色的pNA(p-nitroaniline)，从而可以通过测定吸光度来检测caspase 3的活性。pNA在405nm附近有强吸收。
试剂盒中提供了caspase 3催化产生的黄色产物pNA，可以作为定量caspase 3酶活性的标准品。
本试剂盒用酶标仪检测或容量不超过100μl的分光光度检测杯检测时，除标准曲线外可以检测20个样品。
包装清单：

产品编号 产品名称 包装
C1115-1 裂解液 8ml
C1115-2 检测缓冲液 8ml
C1115-3 Ac-DEVD-pNA(2mM) 200μl
C1115-4 pNA(10mM) 200μl
— 说明书 1份

保存条件：
-20℃保存，Ac-DEVD-pNA和pNA需避光保存。
注意事项：
须自备可以测定A405或A400的酶标仪或容量不超过100μl的分光光度检测杯及相应分光光度计。优先考虑测定A405，如有困难可以测定A400。
Ac-YVAD-pNA需尽量避免反复冻融，请注意适当分装。
测定蛋白浓度需Bradford蛋白浓度测定试剂盒(P0006)，可向碧云天订购。建议样品用水稀释1倍后再用Bradford法测定蛋白浓度，以降低DTT对蛋白浓度测定的干扰。
有文献报道少数类型的细胞凋亡检测不到caspase 3的激活。 
pNA(中文名为4-硝基苯胺)对人体有毒，操作时请特别小心，并注意有效防护以避免直接接触人体或吸入体内。pNA(10mM)在4℃、冰浴等较低温度情况下会凝固而粘在离心管管底、管壁或管盖内，可以20-25℃水浴温育片刻至全部融解后使用。
本试剂盒的裂解液可以和碧云天生产的其它caspase活性检测试剂盒的裂解液通用，即本试剂盒裂解液制备的蛋白样品可以用于碧云天其它caspase活性检测试剂盒的检测。
      本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。 

使用说明：
1.准备工作：
a.裂解液溶解后混匀并置于冰浴上备用。
b.检测缓冲液溶解后混匀并置于冰浴上备用。
2.测定pNA标准曲线：
a.标准品稀释液的配制：按照每0.9ml检测缓冲液加入0.1ml裂解液的比例配制适量的标准品稀释液。
b.把试剂盒提供的pNA (10mM)用标准品稀释液稀释为0、10、20、50、100和200μM，作为标准品。
c.每个浓度取100μl用酶标仪进行检测，或取适当量用容量不超过100μl的分光光度检测杯进行检测，测定A405。
d.每一个标准品的A405减去不含pNA的空白对照的A405计算出实际的因pNA而导致的吸光度，并制作出pNA浓度相对于A405的标准曲线。pNA标准曲线可以参考图1，在0-200μM范围内存在良好的线性关系。


图1.pNA标准曲线。实测数据可能因实验条件、检测仪器等的不同而存在差异，图中数据仅供参考。

3.样品的收集：
a.对于悬浮细胞：把没有诱导凋亡的对照样品和诱导凋亡的样品，600g 4℃离心5分钟收集细胞，小心吸除上清，同时确保尽量没有细胞被吸除，PBS洗涤一次。同前吸尽上清后，按照每200万细胞加入100微升裂解液的比例加入裂解液（如果裂解不充分，可以把裂解液的用量提高至150或200微升），重悬沉淀，冰浴裂解15分钟。下转步骤3d。
b.对于贴壁细胞：吸取细胞培养液，备用。用胰酶消化贴壁细胞，并收集至备用的细胞培养液中。600g 4℃离心5分钟收集细胞，小心吸除上清，同时确保尽量没有细胞被吸除，PBS洗涤一次。同前吸尽上清后，按照每200万细胞加入100微升裂解液的比例加入裂解液（如果裂解不充分，可以把裂解液的用量提高至150或200微升），重悬沉淀，冰浴裂解15分钟。下转步骤3d。
c.对于组织样品：按照每3-10mg组织加入100微升裂解液的比例加入裂解液，在冰浴上用玻璃匀浆器匀浆。然后把匀浆液转移到1.5ml离心管中，冰浴再裂解5分钟。
d.4℃ 16,000-20,000g离心10-15分钟。
e.把上清转移到冰浴预冷的离心管中。
f.立即测定caspase 3的酶活性或-70℃保存样品。同时可以取少量样品用Bradford法测定蛋白浓度，尽量使蛋白浓度达到1-3mg/ml，相当于每10微升待测样品中至少含有10-30μg蛋白。如果细胞较小，可以适当增加细胞的用量。
4.Caspase 3酶活性的检测：
a.取出适量的Ac-DEVD-pNA(2mM)，置于冰浴上备用。
b.如下设置反应体系：

空白对照 样品
检测缓冲液 40μl 40μl
待测样品 0μl 50μl
裂解液 50μl 0μl
Ac-DEVD-pNA(2mM) 10μl 10μl
总体积 100μl 100μl

注意：在设置反应体系时先加检测缓冲液，再加待测样品，适当混匀，注意避免在混匀时产生气泡。随后再加入10μl Ac-DEVD-pNA(2mM)。
c.加入Ac-DEVD-pNA(2mM)后混匀，注意避免在混匀时产生气泡。37℃孵育60-120分钟。发现颜色变化比较明显时即可测定A405。如果颜色变化不明显，可以适当延长孵育时间，甚至可以孵育过夜。
d.样品的A405扣除空白对照的A405，即为样品中caspase 3催化产生的pNA产生的吸光度。通过同步骤1中获得的标准曲线的对比就可以计算出样品中催化产生了多少量的pNA。
e.参考Chemicon公司的caspase 3酶活力单位的定义：One unit is the amount of enzyme that will cleave 1.0nmol of the colorimetric substrate Ac-DEVD-pNA per hour at 37℃ under saturated substrate concentrations。即一个酶活力单位定义为当底物饱和时，在37℃一个小时内可以剪切1nmol Ac-DEVD-pNA产生1nmol pNA的caspase 3的酶量。这样就可以计算出样品中含有多少个酶活力单位的caspase 3。说明：在本试剂盒的检测体系中，底物的起始浓度为 0.2mM，此时底物是饱和的，对于许多样品而言在37℃孵育2个小时以内底物都是饱和的；对于样品中caspase 3酶活力特别高的情况，须用裂解液适当稀释样品后再进行测定。
f.用Bradford法检测待测样品中的蛋白浓度(由于裂解液中含有较高浓度的DTT，不适合采用BCA法进行蛋白浓度测定)。这样就可以计算出一个样品单位重量蛋白中所含的caspase 3的酶活力单位。
常见问题：
1.测定出的A405过低：
A.样品中蛋白含量太低，裂解样品时需设法使样品中的蛋白浓度至少达到1-3mg/ml。
B.样品中激活的caspase水平很低。首先确认凋亡现象是否明显，如果凋亡比较明显并且确认该caspase是可以被激活的，可以适当调节诱导细胞凋亡的时间，希望能找到一个caspase激活比较强的时间点，这样就可以检测出该caspase的激活。可以作一时间曲线，例如诱导凋亡0、2、4、8、16和24小时，或0、1、2、4、8和16小时，或0、1、2、4、6和8小时等。具体的诱导凋亡时间需根据具体情况而定。
2.测定出的A405过高或者样品量不足：
测定出来的A405读数过高时，可以参考下表的反应体系适当减少样品的用量；样品量不足时也可以参考下表减少样品的用量。

空白对照 样品
检测缓冲液 40μl 40μl
待测样品 0μl xμl
裂解液 50μl (50-x)μl
Ac-DEVD-pNA(2mM) 10μl 10μl
总体积 100μl 100μl

说明：其中x不超过50，其余检测方法同上面的使用说明所述。

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79. Xu Y, Zheng H, Kang JS, Zhang L, Su J, Li HY, Sun LK.
ANAT REC 2011 Jun;294(6):945-52. (IF 1.634)
80. Gong L, Liu FQ, Wang J, Wang XP, Hou XG, Sun Y, Qin WD, Wei SJ, Zhang Y, Chen L, Zhang MX.
BBA-BIOMEMBRANES 2011 Jun;1813(6):1211-9. (IF 3.411)
81. Lou H, Fan P, Perez RG, Lou H.
BIOORG MED CHEM LETT 2011 Jul 1;19(13):4021-7. (IF 2.572)
82. Tian LL, Yue W, Zhu F, Li S, Li W.
J Cell Physiol 2011 Jul;226(7):1860-7. (IF 5.546)
83. Shi X, Gu A, Ji G, Li Y, Di J, Jin J, Hu F, Long Y, Xia Y, Lu C, Song L, Wang S, Wang X.
Chemosphere 2011 Oct;85(6):1010-6. (IF 5.778)
84. Du K, Zheng Q, Zhou M, Zhu L, Ai B, Zhou L.
Curr Microbiol 2011 Oct;63(4):341-6. (IF 1.746)
85. Wu Y, Xia ZY, Dou J, Zhang L, Xu JJ, Zhao B, Lei S, Liu HM.
Mol Biol Rep 2011 Oct;38(7):4327-35. (IF 1.402)
86. Abdou MA, He Q, Wen D, Zyaan O, Wang J, Xu J, Baumann AA, Joseph J, Wilson TG, Li S, Wang J.
INSECT BIOCHEM MOLEC 2011 Dec;41(12):938-45. (IF 3.827)
87. Qu M, Zhou Z, Chen C, Li M, Pei L, Chu F, Yang J, Wang Y, Li L, Liu C, Zhang L, Zhang G, Yu Z, Wang D.
Neurochem Int 2011 Dec;59(8):1095-103. (IF 3.881)
88. Tan C, Zhang LY, Chen H, Xiao L, Liu XP, Zhang JX.
BIOCHEM BIOPH RES CO 2011 Dec 16;416(3-4):403-8. (IF 2.985)
89. Liao XH, Chen GT, Li Y, Zhang L, Liu Q, Sun H, Guo H.
RENAL FAILURE 2012;34(5):590-9. (IF 1.985)
90. Hu R, Zhou P, Peng YB, Xu X, Ma J, Liu Q, Zhang L, Wen XD, Qi LW, Gao N, Li P.
PLoS One 2012;7(6):e39664. (IF 2.74)
91. Zhou YC, Liu B, Li YJ, Jing LL, Wen G, Tang J, Xu X, Lv ZP, Sun XG.
EVID-BASED COMPL ALT 2012;2012:385247. (IF 1.813)
92. Li Y, Wang H, Yang B, Yang J, Ruan X, Yang Y, Wakeland EK, Li Q, Fang X.
Int J Biol Sci 2012;8(10):1431-46. (IF 4.858)
93. Guo C, Zeng X, Song J, Zhang M, Wang H, Xu X, Du F, Chen B.
Int J Mol Sci 2012;13(9):11923-40. (IF 4.556)
94. Guo E, He Q, Liu S, Tian L, Sheng Z, Peng Q, Guan J, Shi M, Li K, Gilbert LI, Wang J, Cao Y, Li S.
PLoS One 2012;7(12):e53256. (IF 2.74)
95. Xiao F, Zhang JS, Zhao JY, Wu D.
INT J OPHTHALMOL-CH 2012;5(5):570-5. (IF 1.166)
96. Zha YY, Yang B, Tang ML, Guo QC, Chen JT, Wen LP, Wang M.
INT J NANOMED 2012;7:3099-109. (IF 5.115)
97. Cao M, Tong Y, Lv Q, Chen X, Long Y, Jiang L, Wan J, Zhang Y, Zhang F, Tong N.
PPAR Res 2012;2012:680684. (IF 2.953)
98. Liu Q, Peng YB, Qi LW, Cheng XL, Xu XJ, Liu LL, Liu EH, Li P.
EVID-BASED COMPL ALT 2012;2012:278652. (IF 1.813)
99. Yue R, Hu H, Yiu KH, Luo T, Zhou Z, Xu L, Zhang S, Li K, Yu Z.
PLoS One 2012;7(11):e50778. (IF 2.74)
100. eng Z, Yan H, Hu J, Zhang S, Peng P, Liu Q, Guo D.
PLoS One 2012;7(5):e37700. (IF 2.74)
101. Guo W, Liu W, Hong S, Liu H, Qian C, Shen Y, Wu X, Sun Y, Xu Q.
PLoS One 2012;7(9):e46018. (IF 2.74)
102. Zhang L, Ji Q, Ni ZH, Sun J.
ASIAN PAC J CANCER P  2012;13(8):3803-7. (IF 1.24)
103. Ma EL, Zhao DM, Li YC, Cao H, Zhao QY, Li JC, Sun LX.
J Asian Nat Prod Res 2012;14(9):817-25. (IF 1.345)
105. Li ZL, Liu JC, Hu J, Li XQ, Wang SW, Yi DH, Zhao MG.
J Ethnopharmacol 2012 Jan 31;139(2):388-94. (IF 3.69)
106. Nie X, Song S, Zhang L, Qiu Z, Shi S, Liu Y, Yao L, Zhu D.
PROSTAG OTH LIPID M 2012 Jan;97(1-2):50-9. (IF 2.283)
107. Arockiaraj J, Easwvaran S, Vanaraja P, Singh A, Othman RY, Bhassu S.
FISH SHELLFISH IMMUN 2012 Jan;32(1):161-9. (IF 3.298)
108. Lv L, Jiang SS, Xu J, Gong JB, Cheng Y.
CLIN EXP PHARMACOL P  2012 Jan;39(1):20-7. (IF 2.092)
109. Chen HB, Zhou LZ, Mei L, Shi XJ, Wang XS, Li QL, Huang L.
J NAT MED-TOKYO 2012 Jan;66(1):62-9. (IF 2.055)
110. Yuan XH, Lu CL, Yao N, An LS, Yang BQ, Zhang CL, Ma X.
J Cell Physiol 2012 Jan;227(1):194-203. (IF 5.546)
111. Li Y, Liu L, Niu Y, Feng J, Sun Y, Kong X, Chen Y, Chen X, Gan H, Cao S, Mei Q.
Eur J Nutr 2012 Feb;51(1):107-17. (IF 4.664)
112. Zhao L, Peng DQ, Zhang J, Song JQ, Teng X, Yu YR, Tang CS, Qi YF.
Peptides 2012 Feb;33(2):329-35. (IF 2.843)
113. Li H, Song Y, Zhang LJ, Li FF, Gu Y, Zhang J, Dong WP, Xue L, Zhang LY, Liu F, Wang J, Jiang LN, Ye J, Li Q.
DIABETES-METAB RES  2012 Feb;28(2):145-55.
114. Zhu S, Jin J, Wang Y, Ouyang Z, Xi C, Li J, Qiu Y, Wan J, Huang M, Huang Z.
Food Chem Toxicol 2012 Mar;50(3-4):1149-58. (IF 4.679)
115. Xu HL, Yu XF, Qu SC, Qu XR, Jiang YF, Sui da Y.
Food Chem Toxicol 2012 Mar;50(3-4):590-6. (IF 4.679)
116. Zhai FX, Liu XF, Fan RF, Long ZJ, Fang ZG, Lu Y, Zheng YJ, Lin DJ.
J CANCER RES CLIN 2012 Mar;138(3):439-49. (IF 3.656)
117. Liu Q, Xiao L, Yuan D, Shi X, Li P.
J RECEPT SIG TRANSD 2012 Apr;32(2):120-7. (IF 1.466)
118. Hu W, Chen FH, Yuan FL, Zhang TY, Wu FR, Rong C, Jiang S, Tang J, Zhang CC, Lin MY.
Inflamm Res 2012 Apr;61(4):327-35. (IF 3.174)
119. Bu TL, Jia YD, Lin JX, Mi YL, Zhang CQ.
J ZHEJIANG UNIV-SC B 2012 Apr;13(4):318-26. (IF 2.082)
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Neurosci Lett 2012 May 16;516(2):182-7. (IF 2.274)
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AFR J PHARM PHARMACO 2012 May 22; 6(19): 1428-1434. (IF 0.5)
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J Cell Biochem 2012 May;113(5):1776-86. (IF 4.237)
124. Zhao D, Lin F, Wu X, Zhao Q, Zhao B, Lin P, Zhang Y, Yu X.
Toxicol In Vitro 2012 Jun;26(4):595-602. (IF 2.959)
125. Ma J, Zhang L, Han W, Shen T, Ma C, Liu Y, Nie X, Liu M, Ran Y, Zhu D.
J Lipid Res 2012 Jun;53(6):1093-105. (IF 4.483)
127. Zhang S, Wan Y, Pan T, Gu X, Qian C, Sun G, Sun L, Xiang Y, Wang Z, Shi L.
J Mol Neurosci 2012 Jun;47(2):346-56. (IF 2.678)
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Cell Signal 2012 Jun;24(6):1134-40. (IF 3.968)
131. Zhang W, Zhao L, Liu J, Du J, Wang Z, Ruan C, Dai K.
Thromb Res 2012 Jul;130(1):81-91. (IF 2.869)
132. Lv M, Zhang Y, Liang L, Wei M, Hu W, Li X, Huang Q.
Nanoscale 2012 Jul 7;4(13):3861-6. (IF 6.895)
134. Yang G, Zhou X, Wang J, Zhang W, Zheng H, Lu W, Yuan J.
Food Chem Toxicol 2012 Jul;50(7):2424-31. (IF 4.679)
136. Rong C, Chen FH, Jiang S, Hu W, Wu FR, Chen TY, Yuan FL.
Cell Biol Int 2012 Jul;36(7):635-41. (IF 2.571)
137. Chi J, Zhu Y, Fu Y, Liu Y, Zhang X, Han L, Yin X, Zhao D.
Mol Cell Biochem 2012 Aug;367(1-2):227-36. (IF 2.795)
138. Zhu S, Wang Y, Jin J, Guan C, Li M, Xi C, Ouyang Z, Chen M, Qiu Y, Huang M, Huang Z.
Toxicol In Vitro 2012 Aug;26(5):663-71. (IF 2.959)
139. Jia LQ, Yang GL, Ren L, Chen WN, Feng JY, Cao Y, Zhang L, Li XT, Lei P.
J Ethnopharmacol 2012 Aug 30;143(1):100-8. (IF 3.69)
140. Chen XM, Liu J, Wang T, Shang J.
Toxicol In Vitro 2012 Aug;26(5):649-55. (IF 2.959)
141. Huang P, Zhou ZR, Zheng MQ, Shi FX.
GENET MOL RES 2012 Sep 19;11(4):4775-88. (IF 1.013)
142. Lu B, Xu Y, Xu L, Cong X, Yin L, Li H, Peng J.
ENVIRON TOXICOL CHEM 2012 Sep;34(2):127-35. (IF 3.152)
143. Li XY, Jing CQ, Lei WL, Li J, Wang JJ.
ECOTOX ENVIRON SAFE 2012 Sep;83:102-7. (IF 4.872)
144. Zhang L, Ma J, Shen T, Wang S, Ma C, Liu Y, Ran Y, Wang L, Liu L, Zhu D.
Cell Signal 2012 Oct;24(10):1931-9. (IF 3.968)
145. Ding GB, Liu HY, Lv YY, Liu XF, Guo Y, Sun CK, Xu L.
Chemistry 2012 Oct 29;18(44):14037-46.
147. Wang H, Zhou X, Zhang Y, Zhu H, Zhao L, Fan L, Wang Y, Gang Y, Wu K, Liu Z, Fan D.
FEBS J 2012 Oct;279(19):3652-64. (IF 4.392)
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Cell Biochem Biophys 2012 Nov;64(2):137-45. (IF 2.073)
149. Zhou Z, Wan Y, Zhang Y, Wang Z, Jia R, Fan Y, Nie H, Ying S, Huang P, Wang F.
J Anim Sci 2012 Nov;90(11):3752-61. (IF 2.092)
150. Zhang WF, Xu YY, Xu KP, Wu WH, Tan GS, Li YJ, Hu CP.
Eur J Pharmacol 2012 Nov 5;694(1-3):60-8. (IF 3.263)
151. Liu J, Lu Y, Liang J.
Analyst 2012 Nov 7;137(21):5097-104. (IF 3.978)
152. Wang J, Wang Q, Li J, Shen Q, Wang F, Wang L.
COMP BIOCHEM PHYS C 2012 Nov;156(3-4):195-201. (IF 2.892)
153. Yu W, Wu J, Cai F, Xiang J, Zha W, Fan D, Guo S, Ming Z, Liu C.
PLoS One 2012 December 14; 7(12): e52013. (IF 2.74)
154. Li JZ, Yu SY, Wu JH, Shao QR, Dong XM.
CAN J PHYSIOL PHARM 2012 Dec;90(12):1569-75. (IF 1.946)
155. Zhou BR, Yin HB, Xu Y, Wu D, Zhang ZH, Yin ZQ, Permatasari F, Luo D.
FREE RADICAL RES 2012 Dec;46(12):1458-71. (IF 2.839)
157. Zhang H, Shao D, Wu Y, Cai C, Hu C, Shou X, Dai B, Ye B, Wang M, Jia X.
FISH SHELLFISH IMMUN 2012 Dec;33(6):1229-37. (IF 3.298)
158. Wang J, Wang D, Li Y, Gao Y, Wang S, Zuo H, Xu X, Wang S, Peng R.
RENAL FAILURE 2013;35(5):624-32. (IF 1.985)
159. Cui W, Ma J, Wang X, Yang W, Zhang J, Ji Q.
PLoS One 2013;8(3):e59921. (IF 2.74)
161. Guo J, Zhang Y, Zeng L, Liu J, Liang J, Guo G.
ENVIRON TOXICOL CHEM 2013 Jan;35(1):117-30. (IF 3.152)
162. Guo Z, Zhou B, Liu W, Xu Y, Wu D, Yin Z, Permatasari F, Luo D.
J Dermatol Sci 2013 Jan;69(1):68-76. (IF 3.681)
163. Yin Y, Guan Y, Duan J, Wei G, Zhu Y, Quan W, Guo C, Zhou D, Wang Y, Xi M, Wen A.
Eur J Pharmacol 2013 Jan 15;699(1-3):219-26. (IF 3.263)
164. Qiu L, Chen Y, Gao M, Zheng C, Zhao Q.
J Biomed Mater Res A 2013 Jan;101(1):285-97. (IF 3.525)
165. Fang XY, Chen W, Fan JT, Song R, Wang L, Gu YH, Zeng GZ, Shen Y, Wu XF, Tan NH, Xu Q,
TOXICOL APPL PHARM  2013 Feb 15;267(1):95-103. (IF 3.616)
166. Zhang M, Zhu R, Zhang M, Gao B, Sun D, Wang S.
Res Microbiol 2013 Feb-Mar;164(2):100-9. (IF 3.217)
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Life Sci 2013 Feb 27;92(3):193-201. (IF 3.647)
168. Zhu Y, Chi J, Liu Y, Sun Y, Fu Y, Zhang X, Ding X, Yin X, Zhao D.
Mol Cell Biochem 2013 Feb;374(1-2):113-23. (IF 2.795)
169. Tian R, Tan JT, Wang RL, Xie H, Qian YB, Yu KL.
EUR REV MED PHARMACO 2013 Feb;17(3):349-55. (IF 3.024)
170. Guo LD, Chen XJ, Hu YH, Yu ZJ, Wang D, Liu JZ.
Phytother Res 2013 Mar;27(3):422-30. (IF 4.087)
171. Li D, Liu Z, Zhao W, Zheng X, Wang J, Wang E.
Eur J Pharm Sci 2013 Mar 12;48(4-5):658-67. (IF 3.616)
172. Yang F, Sun X, Shen J, Yu LP, Liang JY, Zheng HQ, Wu ZD.
Parasitol Res 2013 Mar;112(3):1261-72. (IF 1.641)
173. Yao W, Cai X, Liu C, Qin Y, Cheng H, Ji S, Xu W, Wu C, Chen T, Xu J, Long J, Fang Z,
Curr Mol Med 2013 Mar;13(3):417-28. (IF 1.6)
174. Zheng F, He K, Li X, Zhao D, Sun F, Zhang Y, Nie D, Li X, Chu W, Sun Y, Lu Y.
BIOSCIENCE REP 2013 Mar 13;33(2):e00029. (IF 2.942)
175. Li J, Wu F, Chen K, Liang J, Ma S.
J CARDIOVASC PHARM 2013 Apr;61(4):265-71. (IF 2.598)
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Eur J Pharmacol 2013 Apr 5;705(1-3):140-7. (IF 3.263)
177. Li ZL, Hu J, Li YL, Xue F, Zhang L, Xie JQ, Liu ZH, Li H, Yi DH, Liu JC, Wang SW.
FREE RADICAL BIO MED 2013 Apr; 57:132-40. (IF 6.17)
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J ENDODONT 2013 May;39(5):632-7. (IF 3.118)
179. Ma J, Zhang L, Zhang J, Liu M, Wei L, Shen T, Ma C, Wang Y, Chen Y, Zhu D.
INT J BIOCHEM CELL B 2013 Jun;45(6):1031-41. (IF 3.673)
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J BIOCHEM MOL TOXIC 2013 Jun;27(6):330–6. (IF 3.606)
181. Jiang ZQ, Yan XJ, Bi L, Chen JP, Zhou Q, Chen WP.
J Ethnopharmacol 2013 Jul;148(3):851–60. (IF 3.69)
182. Hu M, Xu L, Yin L, Qi Y, Li H, Xu Y, Han X, Peng J, Wan X.
J Appl Toxicol 2013 August; 33(8): 712–722. (IF 2.997)
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Melanoma Res 2013 August; 23 (4):243-253. (IF 2.75)
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Food Chem Toxicol 2013 Aug; 58:495-505. (IF 4.679)
186. Song S, Wang S, Ma J, Yao L, Xing H, Zhang L, Liao L, Zhu D.
Exp Cell Res 2013 August; 319(13):1973-87. (IF 3.383)
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Mol Cell Biochem 2013, Aug;380(1-2):185-93. (IF 2.795)
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Nucleic Acid Ther 2013 August; 23(4):281-288. (IF 4.875)
189. Kun D, Xiang-Lin C, Ming Z, Qi L.
Apoptosis 2013 Sep;18(9):1083-92. (IF 4.543)
190. Qiu Q, Xiong W, Yang C, Gagnon C, Hardy P.
Mol Immunol 2013 Oct;55(3-4):220-30. (IF 3.641)
191. Dan K, Yuehua G, Yanmei Z, Aodi L, Nannan D, Ying P, Yuan Y.
Helicobacter 2013 October;18(5): 347-355. (IF 4.0)
192. Mao Z, Xia W, Wang J, Chen T, Zeng Q, Xu B, Li W, Chen X, Xu S.
J Appl Toxicol 2013 Nov;33(11):1268–76. (IF 2.997)
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Food Chemistry 2013 Nov;141(2):847–52. (IF 5.399)
194. Chen Y, Liu J, Yuan B, Cao C, Qin S, Cao X, Bian G, Wang Z, Jiang J.
MOL CARCINOGEN 2013 Dec;52(12):983-96. (IF 3.825)
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Cell Biochem Biophys 2013 December; 67(3):1239-1248 . (IF 2.073)
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ASIAN PAC J CANCER P  2014;15(6):2753-8. (IF 1.24)
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J Biomed Mater Res A 2014 Mar;102(3):665–73. (IF 3.525)
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Int J Med Sci 2014 Mar 9;11(5):426-35. (IF 2.523)
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Int J Parasitol 2014 Mar;44(3-4):217-24. (IF 3.53)
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J BIOMED NANOTECHNOL 2014 Mar;10(3):455-62. (IF 5.068)
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BMC Cancer 2014 Apr 17;14:271. (IF 3.15)
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EXP BIOL MED 2014 Apr; 239(4):414-22. (IF 3.139)
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TUMOR BIOL  2014 Jun;35(6):5417-25. (IF 3.65)
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EUR REV MED PHARMACO 2014 Jul;18(13):1852-8. (IF 3.024)
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Apoptosis 2014 Jul;19(7):1113-27. (IF 4.543)
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J CARDIOVASC MED 2014 Jul;15(7):579-86. (IF 1.225)
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J Mol Histol 2014 Aug;45(4):473-85. (IF 2.531)
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Neuroreport 2014 August; 25(11): 838–842. (IF 1.394)
210. Chen H, Xing B, Wang L, Weng X, Chen Z, Liu X.
RENAL FAILURE 2014 Aug 26:1-6. (IF 1.985)
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Mol Cell Biochem 2014 Aug;393(1-2):155-64. (IF 2.795)
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Metallomics 2014 Aug;6(8):1502-12. (IF 3.796)
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TOXICOL MECH METHOD 2014 Sep 23:1-9. (IF 2.295)
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J Mol Cell Cardiol 2014 Oct;75:111-21. (IF 4.133)
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Int J Mol Med 2014 Nov;34(5):1430-8. (IF 3.098)
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Mol Neurobiol 2015;51(3):1520-9. (IF 4.5)
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Immunobiology 2015;220(8):947-53. (IF 2.788)
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Journal of Cardiovascular Pharmacology 2015; 65(1):13-21. (IF 2.371)
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Food & function 2015; 6(3):765-771. (IF 3.241)
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Int J Clin Exp Med 2015;8(4):4911-21. (IF 0.166)
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J Lipid Res 2015; 56(4): 836-47. (IF 4.483)
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MOL PHARMACOL 2015; 12(6): 2189-202. (IF 3.664)
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CELL PHYSIOL BIOCHEM 2015;37(3):921-32. (IF 5.5)
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J CARDIOVASC PHARM 2015 Jan;65(1):13-21. (IF 2.598)
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Int J Mol Med 2015 Jan;35(1):218-26. (IF 3.098)
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Cancer Gene Ther 2015 Jan;22(1):23-9. (IF 4.534)
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Mol Med Rep 2015 Jan;11(1):482-8. (IF 2.1)
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Biochemical and Biophysical Research Communications 2015 Jan;456(2):605–9. (IF 2.705)
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Int J Mol Med 2015 Jan;35(1):218-26. (IF 3.098)
236. Y. Yang, H. Meng, Q. Peng, X. Yang, R. Gan, L. Zhao, Z. Chen, J. Lu and Q. H. Meng.
Cancer Gene Ther 2015 Jan;22(1):23-9. (IF 4.534)
237. W. Li, M. Wu, L. Tang, Y. Pan, Z. Liu, C. Zeng, J. Wang, T. Wei and G. Liang.
Toxicology and applied pharmacology 2015 Jan;282(2): 175-83. (IF 3.585)
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Mol Med Rep 2015 Jan;11(1): 482-8. (IF 2.1)
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BIOCHEM BIOPH RES CO 2015 Jan 9;456(2):605-9. (IF 2.985)
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FUND CLIN PHARMACOL 2015 Feb;29(1):54-61. (IF 2.754)
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Fundamental & Clinical Pharmacology 2015 Feb;29(1):54–61. (IF 2.229)
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Oncol Rep 2015 Mar;33(3):1264-74. (IF 3.417)
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C1168S
20-40次
GreenNuc™活细胞Caspase-3活性检测试剂盒
C1157
20次
Caspase 9 活性检测试剂盒
C1116
100次
Caspase 3 活性检测试剂盒
C1077S
20次
活细胞Caspase-3活性与Annexin V细胞凋亡检测试剂盒
C1101
20次
Caspase 1 活性检测试剂盒
C1073S
20次
活细胞Caspase-3活性与线粒体膜电位检测试剂盒
P0006
1000次
Bradford蛋白浓度测定试剂盒
S0033S
>100次
活性氧检测试剂盒
C1062S
20次
Annexin V-FITC细胞凋亡检测试剂盒
C1151
20次
Caspase 8 活性检测试剂盒
C1168M
100-200次
GreenNuc™活细胞Caspase-3活性检测试剂盒
C1086
20次
一步法TUNEL细胞凋亡检测试剂盒(绿色荧光)