细胞凋亡与坏死检测试剂盒
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产品编号 C1056
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¥ 246.00
产品包装:
100次
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碧云天生产的细胞凋亡与坏死检测试剂盒(Apoptosis and Necrosis Assay Kit)为您提供了一种经典而又快速简便的细胞凋亡与细胞坏死检测方法。
本试剂盒采用Hoechst 33342和碘化丙啶(Propidium Iodide,PI)双染的方法。细胞发生凋亡时,染色质会固缩。Hoechst 33342可以穿透细胞膜,染色后凋亡细胞荧光会比正常细胞明显增强。碘化丙啶(PI)不能穿透细胞膜,对于具有完整细胞膜的正常细胞或凋亡细胞不能染色。而对于坏死细胞,其细胞膜的完整性丧失,碘化丙啶(PI)可以染色坏死细胞。上述两种染料双染后,使用流式细胞仪或荧光显微镜检测时,正常细胞为弱红色荧光+弱蓝色荧光,凋亡细胞为弱红色荧光+强蓝色荧光,坏死细胞为强红色荧光+强蓝色荧光。参考下图,左图为诱导凋亡前的正常细胞,右图为诱导凋亡后的细胞。

染色快速方便,两种染料的染色仅需20-30分钟,一步染色即可完成。
使用方便,使用流式细胞仪检测时,无需稀释等配制过程,也无需再准备其它任何溶液。
本试剂盒足够检测100个样品,每个样品的细胞数量可以为10-100万。
包装清单:

产品编号 产品名称 包装
C1056-1 细胞染色缓冲液 100ml
C1056-2 Hoechst染色液 0.5ml
C1056-3 PI染色液 0.5ml
说明书 1份

保存条件:
4℃保存六个月有效,-20℃保存一年有效。Hoechst染色液和PI染色液需避光保存。
注意事项:
需使用流式细胞仪进行红色和蓝色双荧光检测。也可使用荧光显微镜检测。
染色后宜尽快检测。
      Hoechst 33342对人体有害,操作时请小心,并注意有效防护以避免直接接触人体或吸入体内。碘化丙啶(PI)对人体有刺激性,操作时请小心,并注意适当防护以避免直接接触人体或吸入体内。
      本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

使用说明:
1. 每个样品收集约10-100万细胞于1.5ml离心管内,离心弃上清。细胞沉淀用0.8-1毫升细胞染色缓冲液重悬。
2. 加入5微升Hoechst染色液。
3. 加入5微升PI染色液。
4. 混匀,冰浴或4℃孵育20-30分钟。
5. 用流式细胞仪检测红色荧光和蓝色荧光。
6. 如果使用荧光显微镜检测,检测前离心沉淀细胞,用PBS洗涤一次,再涂片观察红色荧光和蓝色荧光。对于贴壁细胞使用荧光显微镜检测,可以不收集细胞,直接依次按照上述比例加入细胞染色缓冲液、Hoechst染色液和PI染色液冰浴或4℃染色20-30分钟。染色后PBS洗涤一次,再在荧光显微镜下观察。
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使用本产品的文献:
1. Yunmao Zhang, Yuhang Han, Xiaoliang Ji, Duyang Zang, Long Qiao, Zhizhi Sheng, Chunyan Wang, Shuli Wang, Miao Wang, Yaqi Hou, Xinyu Chen, Xu Hou
Nature. doi: 10.1038/s41586-022-05124-y. (IF 42.778)
2. Jin S, Zhang QY, Kang XM, Wang JX, Zhao WH.
Ann Oncol 2010;21(2):263-8. (IF 18.274)
3. Dai J, Li Y, Long Z, Jiang R, Zhuang Z, Wang Z, Zhao Z, Lou X, Xia F, Tang BZ
ACS Nano. 14(1):854-866. (IF 14.588)
4. Yi P, Chen G, Zhang H, Tian F, Tan B, Dai J, Wang Q, Deng Z.
Biomaterials 2013 Apr;34(12):3010-9. (IF 10.317)
5. Li C, Du L, Ren Y, Liu X, Jiao Q, Cui D, Wen M, Wang C, Wei G, Wang Y, Ji A, Wang Q
J EXP CLIN CANC RES. 38(1):76. (IF 7.068)
6. Xiaolei Hu, Ling Xu, Xuewei Fu, Jiao Huang, Ping Ji, Zhiwei Zhang, Feng Deng, Xiaomian Wu
Nanoscale. doi: 10.1039/d1nr00696g. (IF 6.895)
7. Zhou ZF, Sun TW, Qin YH, Zhu YJ, Jiang YY, Zhang Y, Liu JJ, Wu J, He SS, Chen F
BIOMATER SCI-UK. 7(12):5044-5053. (IF 6.183)
9. Huang X, Yi C, Fan Y, Zhang Y, Zhao L, Liang Z, Pan J.
MAT SCI ENG C-MATER 2014 Sep 1;42:325-32. (IF 5.88)
10. Huang G, Dong JY, Zhang QJ, Meng QQ, Zhao HR, Zhu BQ, Li SS
Eur J Med Chem. 165:160-171. (IF 5.572)
12. He W, Huang L, Li M, Yang Y, Chen Z, Shen X.
CELL PHYSIOL BIOCHEM 2017;44(2):792-803. (IF 5.5)
13. Ma L, Wang H, Wang C, Su J, Xie Q, Xu L, Yu Y, Liu S, Li S, Xu Y, Li Z.
Aging Dis 2016 May 27;7(3):254-66. (IF 5.402)
14. Li G, Ye L, Pan J, Long M, Zhao Z, Yang H, Tian J, Wen Y, Dong S, Guan J, Luo B.
Liver Int 2012 Jul;32(6):998-1007. (IF 5.175)
15. Wang J, Qi L, Mei L, Wu Z, Wang H C.
Int J Biol Macromol 2016 Jul;88:81-7. (IF 5.162)
17. Chang L, Wang Z, Ma F, Tran B, Zhong R, Xiong Y, Dai T, Wu J, Xin X, Guo W, Xie Y, Mao Y, Zhu YZ
Oxid Med Cell Longev. 2019:6173685. (IF 5.076)
18. Banerjee J, Ghose J, Sinha M, Sen S.
Oxid Med Cell Longev. 2019:3764252. (IF 5.076)
19. Jing Wang, Wei Zhang, Sixin Wang, Yamin Wang, Xu Chu, Haifeng Ji
Oxid Med Cell Longev. doi: 10.1155/2021/8936907. (IF 5.076)
20. Gu-Hua Tang, Jing-Hong Liu, Xin-Yuan Sun, Jian-Ming Ouyang
Oxid Med Cell Longev. doi: 10.1155/2022/2082263. (IF 5.076)
21. Ma W, Xu W, Xu H, Chen Y, He Z, Ma M.
Planta 2010 Jul;232(2):325-35. (IF 3.39)
22. Song J, Guo X, Xie X, Zhao X, Li D, Deng W, Song Y, Shen F, Wu M, Wei L.
J Cell Biochem 2011 Nov;112(11):3406-20. (IF 4.237)
23. Meng Q, Lv J, Ge H, Zhang L, Xue F, Zhu Y, Liu P.
Mol Biol Rep 2012 May;39(5):5867-73. (IF 1.402)
24. Tang S, Hu J, Meng Q, Dong X, Wang K, Qi Y, Chu C, Zhang X, Hou L.
Cell Biochem Biophys 2013 Mar;65(2):197-202. (IF 2.073)
25. Wang B, Yu T, Dong X, Zhang Z, Song L, Xu Y, Zhang XH.
Vet Microbiol 2013 May 3;163(3-4):282-9. (IF 3.03)
26. Yin X, Zhou X, Xue Z, Tian L, Zhou Y, Yang L, Zhao D.
Mol Cell Biochem 2014 Jan;385(1-2):43-52. (IF 2.795)
27. Cai XY, Xiong LM, Yang SH, Shao ZW, Xie M, Gao F, Ding F.
Spine J 2014 Mar 1;14(3):483-90. (IF 3.191)
28. Zhu W, Yang B, Fu H, Ma L, Liu T, Chai R, Zheng Z, Zhang Q, Li G.
BIOCHEM BIOPH RES CO 2015 Mar 13;458(3):590-5. (IF 2.985)
29. Han Y, Shao Y, Liu T, Qu YL, Li W, Liu Z.
PLoS One 2015 Apr 8;10(4):e0122951. (IF 2.74)
30. Chen F, Li Q, Zhang Z, Lin P, Lei L, Wang A, Jin Y.
Int J Mol Sci 2015 Aug 20;16(8):19780-95. (IF 4.556)
31. Sun Y, Zhang J, Zhou J, Huang Z, Hu H, Qiao M, Zhao X, Chen D.
Eur J Pharmacol 2015 Dec 5;768:28-40. (IF 3.263)
33. Chen F, Lin P, Wang N, Yang D, Wen X, Zhou D, Wang A, Jin Y.
Toxicol In Vitro 2016 Apr;32:115-22. (IF 2.959)
35. Wang Y, Wu Y, Wang Y, Fu A, Gong L, Li W, Li Y.
APPL MICROBIOL BIOT 2016 Dec 12. [Epub ahead of print] (IF 3.53)
36. Wang Y, Wang X, Shi J, Zhu R, Zhang J, Zhang Z, Ma D, Hou Y, Lin F, Yang J.
SCI REP-UK 2016 Dec 20;6:39477. (IF 3.998)
37. Yi T, Wu X, Long Z, Duan G, Wu Z, Li H, Chen H, Zhou X.
Biomed Res Int 2017;2017:1314297. (IF 2.276)
38. Jin X, Yu ZF, Chen F, Lu GX, Ding XY, Xie LJ, Sun JT.
Front Cell Neurosci 2017 Mar 7;11:66. (IF 3.921)
39. Wang Y, Wu Y, Wang Y, Fu A, Gong L, Li W, Li Y.
APPL MICROBIOL BIOT 2017 Apr;101(7):3015-3026. (IF 3.53)
40. Song C, Luo B, Gong L.
PLoS One 2017 Apr 13;12(4):e0175009. (IF 2.74)
42. Long Z, Duan G, Li H, Yi T, Wu X, Chen F, Wu Z, Gao Y.
PLoS One 2017 Jul 27;12(7):e0181903. (IF 2.74)
43. Pang J, Zhou Q, Sun X, Li L, Zhou B, Zeng F, Zhao Y, Shen W, Sun Z.
TOXICOL APPL PHARM  2017 Oct 15;333:60-67. (IF 3.616)
44. Sun Q, Wang J, Li Y, Zhuang J, Zhang Q, Sun X, Sun D.
Chem Biol Drug Des 2017 Nov;90(5):1019-1028. (IF 2.548)
45. Du J, Zhang P, Gan M, Zhao X, Xu Y, Li Q, Jiang Y, Tang G, Li M, Wang J, Li X, Zhang S, Zhu L
Gene 2018 Aug 20;668:1-7. (IF 2.984)
46. Ma J, Zhang J, Wang Y, Long K, Wang X, Jin L, Tang Q, Zhu L, Tang G, Li X, Li M
Gene 2018 Oct 30;675:36-43. (IF 2.984)
47. Zhu K, Li K, Wang H, Kang L, Dang C, Zhang Y
Pharmacology. 104(3-4):113-125. (IF 1.625)
48. Le L, Fu H, Lv Q, Bai X, Zhao Y, Xiang J, Jiang B, Hu K, Chen S
Phytomedicine. 53:193-204. (IF 4.268)
49. Yang X, Zhang Y, Lai W, Xiang Z, Tu B, Li D, Nan X, Chen C, Hu Z, Fang Q
Nanotoxicology. 13(1):35-49. (IF 4.925)
50. Wu J, Liu H, Chu T, Jiang P, Li ST
J Muscle Res Cell Motil. 40(1):43-51.
51. Li W, Yue X, Li F
ASIAN AUSTRAL J ANIM. 32(5):629-636. (IF 1.664)
52. Zhang J, Qiu W, Ma J, Wang Y, Hu Z, Long K, Wang X, Jin L, Tang Q, Tang G, Zhu L, Li X, Shuai S, Li M
Int J Mol Sci. 20(10). pii: E2418. (IF 4.556)
53. Wang JR, Luo YH, Piao XJ, Zhang Y, Feng YC, Li JQ, Xu WT, Zhang Y, Zhang T, Wang SN, Xue H, Wang WZ, Cao LK, Jin CH
Drug Dev Res. 80(4):461-470.
54. He C, Zhao Y, Jiang X, Liang X, Yin L, Yin Z, Geng Y, Zhong Z, Song X, Zou Y, Li L, Zhang W, Lv C
Int Immunopharmacol. 71:328-335. (IF 3.943)
55. Wang JR, Shen GN, Luo YH, Piao XJ, Zhang Y, Wang H, Li JQ, Xu WT, Zhang Y, Wang SN, Zhang T, Xue H, Cao LK, Jin CH
J Chemother. 31(4):214-226.
56. Yang Q, Bai SY, Li LF, Li S, Zhang Y, Munir M, Qiu HJ
J Virol. 93(16). pii: e00718-19. (IF 4.501)
57. Zhou XL, Chen ZD, Zhou YM, Shi RH, Li ZJ
Molecules. 24(17). pii: E3092. (IF 3.267)
58. Yang Y, Wang C, Zhuge Y, Zhang J, Xu K, Zhang Q, Zhang H, Chen H, Chu M, Jia C
Front Microbiol. 10:1810. (IF 4.235)
59. Wang H, Cheng J, Wang H, Wang M, Zhao J, Wu Z
J Food Biochem. 43(12):e13052. (IF 1.662)
60. Sun N, Liang Y, Chen Y, Wang L, Li D, Liang Z, Sun L, Wang Y, Niu H
Int J Mol Med. 44(6):2189-2200. (IF 3.098)
62. Zhou J, Li G, Han G, Feng S, Liu Y, Chen J, Liu C, Zhao L, Jin F
INVEST NEW DRUG. 38(1):50-59. (IF 3.525)
63. Yiyi Li, Xuan Niu, Haitao Xu, Qingwen Li, Lanxia Meng, Mingyang He, Jie Zhang, Zhentao Zhang, Zhaohui Zhang
Exp Cell Res. doi: 10.1016/j.yexcr.2020.111847. (IF 3.383)
64. Wen-Hui Xie, Jian Ding, Xiao-Xia Xie, Xiao-Huang Yang, Xiao-Fan Wu, Zhi-Xin Chen, Qi-Lan Guo, Wen-Yu Gao, Xiao-Zhong Wang, Dan Li
Inflamm Res. doi: 10.1007/s00011-020-01351-z. (IF 3.174)
65. Mengjie Wang, Jingwen Tan, Chenglan Jiang, Shuting Li, Xinan Wu, Guanghui Ni, Yuefeng He
Environ Geochem Health. doi: 10.1007/s10653-020-00740-x.
66. Hongchao Gou, Zhibiao Bian, Rujian Cai, Pinpin Chu, Shuai Song, Yan Li, Zhiyong Jiang, Kunli Zhang, Dongxia Yang, Chunling Li
Front Microbiol. doi: 10.3389/fmicb.2021.664353. (IF 4.235)
67. Jie Tang, Bo Hu, Huaping Zheng, Xiaolan Qian, Yi Zhang, Jia Zhu, Guangtao Xu, Deqing Chen, Xin Jin, Wanlu Li, Long Xu
Ecotoxicol Environ Saf. doi: 10.1016/j.ecoenv.2021.112423.
68. Jingjing Li, Chaowu Yang, Peng Ren, Zhongzhen Lin, Donghao Zhang, Xiaosong Jiang, Li Wang, Yiping Liu
Mol Biol Rep. doi: 10.1007/s11033-021-06831-x. (IF 1.402)
69. Wenhuan Guo, Zehua Zeng, Cencan Xing, Jinghui Zhang, Wangyu Bi, Jingjie Yang, Roshan Shah, Donghui Wang, Yingxian Li, Xiaoshuang Zhang, Yongzhong Bian, Hongwu Du
Cytotherapy. doi: 10.1016/j.jcyt.2021.07.018. (IF 4.218)
70. Jing Sun, Yanshu Zhang, Licheng Yan, Si Liu, Weicheng Wang, Yi Zhu, Weixuan Wang, Shuang Li, Bin He, Lei Wu, Lijin Zhang
J Inorg Biochem. doi: 10.1016/j.jinorgbio.2022.111792. (IF 3.212)
71. Yaqiong An, Haibo Li, Mengxiao Wang, Zhaohua Xia, Lexi Ding, Xiaobo Xia
Biomed Pharmacother. doi: 10.1016/j.biopha.2022.113378. (IF 4.545)
72. Ruonan Gao, Zhou Chen, Yubin Wu, Ruiyu Chen, Wenrong Zheng, Liqin Qi, Xiaoying Liu, Xiaohong Liu, Libin Liu
Free Radic Biol Med. doi: 10.1016/j.freeradbiomed.2022.10.313.
73. Shunlian Hu, Lu Wang, Yahan Xu, Fajiu Li, Tao Wang
Respir Res. doi: 10.1186/s12931-022-02279-0.
74. Min Li, Shengguang Wang, Ce Zhang, Chenglin Chi, Rongxia Liu, Tian Wang, Fenghua Fu
Int Immunopharmacol. doi: 10.1016/j.intimp.2022.109659. (IF 3.943)
75. Xiancheng Qiu, Qianke Tao, Lihan Zhang, Chenghao Kuang, Yuke Xie, Lifang Zhang, Shigang Yin, Jianhua Peng, Yong Jiang
J Neurochem. doi: 10.1111/jnc.15751. (IF 4.066)