DiO (细胞膜绿色荧光探针)
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产品编号 ： C1038
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¥ 472.00
产品包装:
10mg
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DiO即DiOC18(3)，全称为3,3′-dioctadecyloxacarbocyanine perchlorate，是最常用的细胞膜荧光探针之一，呈现绿色荧光。DiO是一种亲脂性膜染料，进入细胞膜后可以侧向扩散逐渐使整个细胞的细胞膜被染色。
DiO在进入细胞膜之前荧光非常弱，仅当进入到细胞膜后才可以被激发出很强的荧光。DiO被激发后可以发出绿色的荧光，DiO和磷酯双层膜结合后的激发光谱和发射光谱参考下图。其中，最大激发波长为484nm，最大发射波长为501nm。

DiO的分子式为C53H85ClN2O6，分子量为881.72，CAS number为34215-57-1。
DiO可以溶解于无水乙醇、DMSO和DMF，其中在DMSO中溶解度大于10mg/ml。发现较难溶解时可以适当加热，并用超声处理以促进溶解。
DiO被广泛用于正向或逆向的，活的或固定的神经等细胞或组织的示踪剂或长期示踪剂(long-term tracer)。DiO通常不会明显影响细胞的生存力(viability)。DiO对于细胞膜染色的荧光强度通常要低于DiI，有时对于某些经过固定的组织的染色效果欠佳。
DiO除了最简单的细胞膜荧光标记外，还可以用于检测细胞的融合和粘附，检测发育或移植过程中细胞迁移，通过FRAP(Fluorescence Recovery After Photobleaching)检测脂在细胞膜上的扩散，检测细胞毒性和标记脂蛋白等。
用于细胞膜荧光标记时，DiO的常用浓度为1-30μM，最常用的浓度为5-10μM。DiO可以直接染色活的细胞或组织，染色时间通常为5-20分钟。对于固定的细胞或组织，通常宜使用配制在PBS中的4%多聚甲醛进行固定，使用其它不适当的固定液会导致荧光背景较高。
包装清单：

产品编号 产品名称 包装
C1038 DiO (细胞膜绿色荧光探针) 10mg
— 说明书 1份

保存条件：
4℃避光保存，一年有效。配制的储存液-20℃避光保存，半年有效。
注意事项：
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
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C1036
10mg
DiI (细胞膜红色荧光探针)
C1993S
200-1000次
细胞膜绿色荧光染色试剂盒(DiO)
C1039-10mg
10mg
DiD (细胞膜远红荧光探针)
C1991S
200-1000次
细胞膜红色荧光染色试剂盒(DiI)
C1995S
200-1000次
细胞膜远红荧光染色试剂盒(DiD)
C1419S
100次
Beyo3D™细胞膜绿色荧光染色试剂盒(DiO)
C1047S
50μl
Lyso-Tracker Green (溶酶体绿色荧光探针)
C1005
10ml
DAPI染色液
C1046
50μl
Lyso-Tracker Red (溶酶体红色荧光探针)
C1002
5mg/ml×0.2ml
DAPI
C2201S
0.2ml
Actin-Tracker Green-488 (微丝绿色荧光探针)
C1048
50μg
Mito-Tracker Green (线粒体绿色荧光探针)