Actin-Tracker Green (微丝绿色荧光探针)
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产品编号 ： C1033
数量 ：
¥ 486.00 元
产品包装:
0.2ml
产品简介
使用说明
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本产品已停产，推荐选购类似产品：C2201S Actin-Tracker Green-488 (微丝绿色荧光探针)。
Actin-Tracker Green是一种Actin绿色荧光探针，可以用于培养细胞或组织切片的Actin特异性荧光染色。
Actin-Tracker Green探针为荧光染料FITC标记的毒蕈肽(phalloidin)，即phalloidin-FITC，其分子量约为1251.4，最大激发波长为496nm，最大发射波长为516nm。
本产品可以用于细胞或组织内的微丝(microfilament)的荧光检测。HeLa细胞使用本产品染色的效果参见图1。


图1. HeLa细胞使用本产品的染色效果图。图中可见微丝的绿色细丝状形态的染色。图中的染色实验在六孔板中进行，Actin-Tracker Green的稀释比例为1:100。本图仅作参考，不同的样品不同的检测条件，实际获得的结果可能有所差别。

本产品使用时的推荐稀释比例为1:40-1:200。如果每个片子需要使用200μl染色工作液，每个包装的本产品足够用于40-200个片子的染色。
包装清单：

产品编号 产品名称 包装
C1033 Actin-Tracker Green 0.2ml
－ 说明书 1份

保存条件：
-20℃避光保存，一年有效。
注意事项：
为避免反复冻融，建议适当分装。
对于微量的液体，每次使用前先离心数秒钟，使液体充分沉降到管底。
荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
需自备盖玻片和载玻片(可以向碧云天订购)。
Phalloidin 有一定毒性(LD50为2mg/kg体重)，1ml Actin-Tracker Green中的phalloidin少于0.2mg，使用时请注意适当防护。
本产品仅限于专业人员的科学研究用，不得用于临床诊断或治疗，不得用于食品或药品，不得存放于普通住宅内。
为了您的安全和健康，请穿实验服并戴一次性手套操作。

使用说明：
1. 固定细胞或组织切片的荧光染色：
a. 用PBS洗涤细胞或组织切片2次。
b. 用碧云天的免疫染色固定液(P0098)或PBS配制的3.7%甲醛溶液室温固定细胞或组织切片约10分钟。注意：甲醇可以破坏actin蛋白，因此不能使用含有甲醇的固定液。并且尽量使用不含甲醇的甲醛。
c. 用碧云天的免疫染色洗涤液(P0106)或含0.1% Triton X-100的PBS洗涤2-4次，每次约5分钟。
d. 用碧云天的免疫荧光染色二抗稀释液(P0108)或含有1-5%BSA和0.1% Triton X-100的PBS按照1:40-1:200的比例稀释Actin-Tracker Green，例如5μl或25μl Actin-Tracker Green用1ml稀释液稀释，稀释后的溶液即为Actin-Tracker Green染色工作液。注：稀释比例可以根据实际染色效果进行适当调整。
e. 把Actin-Tracker Green染色工作液按照每个片子约200μl的比例滴加到片子上，室温避光孵育20-60分钟。为防止蒸发，孵育时最好将片子置于载玻片染色盒中，载玻片染色盒(FSR958)可以向碧云天订购。
f. 用碧云天的免疫染色洗涤液(P0106)或含0.1% Triton X-100的PBS洗2-4次，每次约5分钟。
g. 随后可以直接用荧光显微镜进行观察。为长期保存，可以在片子自然干燥后封片并于4℃避光保存，保存时间可长达6个月左右。
2. 对于活细胞的荧光染色：
Phalloidin通常不具有细胞通透性，因此极少用于活细胞染色。但也有报道活细胞可被标记，可能是通过细胞的吞噬作用进入了某些细胞。一般对于活细胞的染色需要更多的染料。
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C2201S
0.2ml
Actin-Tracker Green-488 (微丝绿色荧光探针)
C2207S
0.2ml
Actin-Tracker Red-Rhodamine (微丝红色荧光探针)
C2203S
0.2ml
Actin-Tracker Red-555 (微丝红色荧光探针)
C2205S
0.2ml
Actin-Tracker Red-594 (微丝红色荧光探针)
C1005
10ml
DAPI染色液
C1002
5mg/ml×0.2ml
DAPI
C2213S
20-200次
微管绿色荧光染色试剂盒(活细胞染色用)
C1050
40μl
Tubulin-Tracker Red (抗体法微管红色荧光探针)
C2006
>100次
线粒体膜电位检测试剂盒(JC-1)
C1038
10mg
DiO (细胞膜绿色荧光探针)
C1036
10mg
DiI (细胞膜红色荧光探针)
P0108
100ml
免疫荧光染色二抗稀释液