DAPI
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产品编号 C1002
数量
¥ 257.00
产品包装:
5mg/ml×0.2ml
产品简介
使用说明
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DAPI,即2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride,也称DAPI dihydrochloride,分子式为C16H15N5·2HCl,分子量为350.25,CAS Number 28718-90-3。
DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比,对双链DNA的染色灵敏度要高很多倍。
DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
DAPI的最大激发波长为340nm,最大发射波长为488nm;DAPI和双链DNA结合后,最大激发波长为364nm,最大发射波长为454nm。
本DAPI溶液用水配制,浓度为5mg/ml。
用于细胞核染色时,推荐的DAPI工作浓度为0.5-10μg/ml。
包装清单:

产品编号 产品名称 包装
C1002 DAPI 5mg/ml×0.2ml
说明书 1份

保存条件:
-20℃避光保存,一年有效。
注意事项:
荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。
为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液(P0126)可以向碧云天订购。
DAPI对人体有刺激性,操作时请小心,并注意适当防护以避免直接接触人体或吸入体内。
本产品仅限于专业人员的科学研究用,不得用于临床诊断或治疗,不得用于食品或药品,不得存放于普通住宅内。
为了您的安全和健康,请穿实验服并戴一次性手套操作。

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Analyst 2013 Jan 21;138(2):666-70. (IF 3.978)
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Exp Ther Med 2013 Jan;5(1):77-84. (IF 1.785)
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Neuroscience 2013 Feb 12;231:353-62. (IF 3.056)
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Cell Death Dis 2013 Feb 7;4:e481. (IF 6.304)
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J MATER CHEM B 2013 Feb;14:1939-46. (IF 5.344)
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Peptides 2013 Feb;40:104-11. (IF 2.843)
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Mol Pharmacol 2013 Feb;83(2):389-98. (IF 3.664)
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Clin Vaccine Immunol 2013 Feb;20(2):287-94. (IF 3.233)
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FEBS Lett 2013 Feb 14;587(4):378-85. (IF 3.057)
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Neurol Sci 2013 Mar;34(3):357-64. (IF 2.415)
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Exp Cell Res 2013 Mar 10;319(5):740-9. (IF 3.383)
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Food Chem Toxicol 2013 Mar;53:62-8. (IF 4.679)
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J Neurosci 2013 Mar 6;33(10):4265-79. (IF 5.673)
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Neurochem Int 2013 Mar;62(4):458-67. (IF 3.881)
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Lab Chip 2013 Mar 7;13(5):860-5. (IF 6.774)
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INT J PHARMACOL 2013 Apr 15;447(1-2):281-92. (IF 0.692)
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CHINESE SCI BULL 2013 Apr;20(542):2095-9273. (IF 1.649)
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COLLOID SURFACE B 2013 Apr 20;110C:138-147. (IF 4.389)
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FREE RADICAL BIO MED 2013 Apr;57:132-40. (IF 6.17)
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PLoS One 2013 Apr 23;8(4):e61345. (IF 2.74)
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APPL BIOCHEM BIOTECH 2013 Apr;169(8):2273-80. (IF 2.277)
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Cell Reprogram 2013 Apr;15(2):134-42. (IF 1.702)
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ACTA BIOCH BIOPH SIN 2013 Apr;45(4):259-67. (IF 2.836)
163. Wu HJ, Liu C, Duan WX, Xu SC, He MD, Chen CH, Wang Y, Zhou Z, Yu ZP, Zhang L, Chen Y.
MUTAT RES-REV MUTAT 2013 Apr 15;752(1-2):57-67. (IF 5.803)
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PLoS One 2013 Apr 23;8(4):e61508. (IF 2.74)
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Cell Reprogram 2013 Apr;15(2):159-65. (IF 1.702)
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PLoS One 2013 Apr 30;8(4):e62363. (IF 2.74)
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Metabolism 2013 May;62(5):743-52. (IF 6.159)
168. Sun B, Cai Y, Li Y, Li J, Liu K, Li Y, Yang Y.
Virology 2013 May 25;440(1):75-83. (IF 2.819)
169. Wang Y, Liu P, Qiu L, Sun Y, Zhu M, Gu L, Di W, Duan Y.
Biomaterials 2013 May;34(16):4068-77. (IF 10.317)
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Parasitology 2013 May;140(6):746-55. (IF 2.783)
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Vet Res 2013 May 7;44(1):29. (IF 3.357)
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Int J Mol Sci 2013 May 27;14(6):11224-37. (IF 4.556)
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Fish & Shellfish Immunology 2013 May;34(5):1279–86. (IF 3.298)
174. Yu S, Zhao H, Wang H, Wang X, Shao G, Xu L, Si W, Chen L, Zhang W, Liu S.
J Virol Methods 2013 May;189(2):355-61. (IF 1.786)
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J CARDIOVASC PHARM 2013 May;61(5):391-400. (IF 2.598)
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Int J Biol Macromol 2013 May;56:106-13. (IF 5.162)
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Biomaterials 2013 Jun;34(18):4404-17. (IF 10.317)
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Chinese Journal of Polymer Science 2013 Jun;31(5):912-23. (IF 2.804)
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Biomaterials 2013 Jun;34(18):4520-31. (IF 10.317)
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Apoptosis 2013 Jun;18(6):751-65. (IF 4.543)
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Int J Oncol 2013 Jun;42(6):2094-102. (IF 3.899)
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BIOCHEM BIOPH RES CO 2013 Jun 14;435(4):640-5. (IF 2.985)
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Insect Mol Biol 2013 Jun;22(3):273-83. (IF 2.533)
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TUMOR BIOL  2013 Jun;34(3):1759-66. (IF 3.65)
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Cell Reprogram 2013 Jun;15(3):195-205. (IF 1.702)
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Int J Colorectal Dis 2013 Jul;28(7):933-40. (IF 2.108)
190. Pan B, Yu B, Ren H, Willard B, Pan L, Zu L, Shen X, Ma Y, Li X, Niu C, Kong J, Kang S, Eugene Chen Y, Pennathur S, Zheng L.
FREE RADICAL BIO MED 2013 Jul;60:272-81. (IF 6.17)
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J Pharm Pharmacol 2013 Aug;65(8):1107-17. (IF 2.571)
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Cancer Lett 2013 Aug 9;336(1):231-9. (IF 7.36)
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Chinese Chemical Letters 2013 Aug;24(8):710–4. (IF 3.839)
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MAT SCI ENG C-MATER 2013 Aug 1;33(6):3514-21. (IF 5.88)
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Acta Histochem 2013 Sep;115(7):692-7. (IF 2.107)
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Int J Clin Oncol 2013 Oct;18(5):856-63. (IF 2.879)
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Journal of Materials Science 2013 Oct;48(19):6567-77. (IF 3.442)
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Inflammation 2013 Oct;36(5):1064-74. (IF 3.212)
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Helicobacter 2013 Oct;18(5):347-55. (IF 4.0)
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Curr Eye Res 2013 Oct;18(5):347-55. (IF 1.754)
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Int J Cardiol 2013 Oct 3;168(3):2109-19. (IF 3.229)
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Neurotox Res 2013 Nov;24(4):512-22. (IF 2.992)
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AGE 2013 Dec;35(6):2237-53. (IF 4.648)
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TISSUE ENG PART C-ME 2013 Dec;19(12):925-36. (IF 2.608)
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REPROD FERT DEVELOP 2014;26(4):533-9. (IF 1.718)
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Oral Dis 2014 Jan;20(1):100-8. (IF 2.613)
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BIOCHEM BIOPH RES CO 2014 Mar 28;446(1):105-12. (IF 2.985)
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FUND CLIN PHARMACOL 2014 Apr;28(2):180-9. (IF 2.754)
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Cell Biol Int 2015 Jan;39(1):35-43. (IF 2.571)
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Andrology 2015 Mar;3(2):332-44. (IF 3.106)
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J Cell Physiol 2015 Mar;230(3):680-90. (IF 5.546)
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Liver Int 2015 Apr;35(4):1373-82. (IF 5.175)
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Am J Cancer Res 2015 Aug 15;5(9):2643-59. (IF 5.177)
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Birth Defects Research Part A Clinical and Molecular Teratology 2015 Sep;103(9):772-9. (IF 1.94)
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PLoS One 2015 Sep 29;10(9):e0139322. (IF 2.74)
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Anim Sci J 2016 Jan;87(1):84–91. (IF 1.399)
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PLoS One 2016 Mar 30;11(3):e0152599. (IF 2.74)
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J MATER SCI-MATER M 2016 Apr;27(4):77. (IF 2.489)
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Oncol Rep 2016 Apr;35(4):1916-24 (IF 3.417)
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Heart Vessels 2016 Aug;31(8):1370-9. (IF 1.618)
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Theriogenology 2016 Aug;86(3):868-78. (IF 2.094)
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SCI REP-UK 2016 Aug 31;6:32257. (IF 3.998)
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Int J Med Sci 2016 Sep 20;13(10):765-771. (IF 2.523)
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MED SCI MONITOR 2016 Oct 7;22:3595-3604. (IF 1.918)
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BIOCHEM BIOPH RES CO 2016 Oct 28;479(4):670-675. (IF 2.985)
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J MOL MED 2016 Nov;94(11):1267-1279. (IF 4.427)
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Dis Esophagus 2016 Nov;29(8):1144-1151. (IF 2.386)
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Curr Eye Res 2016 Dec 2:1-7. (IF 1.754)
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Exp Mol Pathol 2016 Dec 7;102(1):7-14. (IF 2.28)
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J Ophthalmol 2017;2017:6067890. (IF 1.447)
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Exp Cell Res 2017 Jan 15;350(2):301-311. (IF 3.383)
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Reprod Biomed Online 2017 Feb;34(2):191-202. (IF 3.218)
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Int J Oncol 2017 Feb;50(2):505-514. (IF 3.899)
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Front Pharmacol 2017 Mar 17;8:135. (IF 4.225)
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Exp Cell Res 2017 Mar 1;352(1):95-103. (IF 3.383)
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J Cancer 2017 Mar 12;8(6):983-992. (IF 3.565)
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